Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram Semen
ABSTRACT Objective This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl2), zinc oxide nanoparticles (ZnO‐NPs) and Curc loaded on ZnO‐NPs (Curc‐co‐ZnO‐NPs) in cryopreservation dilution affected the quality of ram sperm after thawing. Methods ZnO‐NPs were synthesis...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70091 |
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| author | Fatemeh Omidi Hadi Hajarian Hamed Karamishabankareh Leila Soltani Mojtaba Dashtizad |
| author_facet | Fatemeh Omidi Hadi Hajarian Hamed Karamishabankareh Leila Soltani Mojtaba Dashtizad |
| author_sort | Fatemeh Omidi |
| collection | DOAJ |
| description | ABSTRACT Objective This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl2), zinc oxide nanoparticles (ZnO‐NPs) and Curc loaded on ZnO‐NPs (Curc‐co‐ZnO‐NPs) in cryopreservation dilution affected the quality of ram sperm after thawing. Methods ZnO‐NPs were synthesised using Berberis vulgaris leaf aqueous extract. Then, Curc was loaded on the ZnO‐NPs that had been synthesised. We used analytical methods to look at the composition, morphology and size of green synthesised ZnO‐NPs and Curc‐co‐ZnO‐NPs, including UV‐Vis, zeta potential, EDX, DLS, FE‐SEM and FT‐IR. Using a Tris‐base extender containing various concentrations of Curc, ZnCl2, ZnO‐NPs and Curc‐co‐ZnO‐NPs (0, 1, 10 and 100 µg/mL), semen samples from four rams were combined. Sperm motility, viability, DNA and plasma membrane integrity, total abnormalities and malondialdehyde (MDA) generation were all evaluated in treatment groups after thawing. Results The results showed that adding 1 µg/mL of ZnO‐NPs and Curc‐co‐ZnO‐NPs significantly reduced the level of MDA and total abnormalities (p < 0.05). Additionally, following the freeze‐thawing procedure, the presence of 1 µg/mL of Curc‐co‐ZnO‐NPs in the diluent of ram sperm significantly increased the percentage of sperm viability and motility in comparison to the control and other treatment groups (p < 0.05). Furthermore, as compared to the control group and other treatments, treatments containing 1 µg/mL of Curc‐co‐ZnO‐NPs significantly improved membrane and DNA integrity (p < 0.05). Conclusions It appears that following freeze‐thawing, the Curc‐co‐ZnO‐NPs (1 µg/mL) enhanced sperm parameters. |
| format | Article |
| id | doaj-art-119ba71ce84947c4ab8b10634ce6c5c5 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-119ba71ce84947c4ab8b10634ce6c5c52025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70091Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram SemenFatemeh Omidi0Hadi Hajarian1Hamed Karamishabankareh2Leila Soltani3Mojtaba Dashtizad4Department of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah IranDepartment of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah IranDepartment of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah IranDepartment of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah IranDepartment of Animal Science National Institute of Genetics and Biotechnology Tehran IranABSTRACT Objective This study looked at how different concentrations of curcumin (Curc), zinc chloride (ZnCl2), zinc oxide nanoparticles (ZnO‐NPs) and Curc loaded on ZnO‐NPs (Curc‐co‐ZnO‐NPs) in cryopreservation dilution affected the quality of ram sperm after thawing. Methods ZnO‐NPs were synthesised using Berberis vulgaris leaf aqueous extract. Then, Curc was loaded on the ZnO‐NPs that had been synthesised. We used analytical methods to look at the composition, morphology and size of green synthesised ZnO‐NPs and Curc‐co‐ZnO‐NPs, including UV‐Vis, zeta potential, EDX, DLS, FE‐SEM and FT‐IR. Using a Tris‐base extender containing various concentrations of Curc, ZnCl2, ZnO‐NPs and Curc‐co‐ZnO‐NPs (0, 1, 10 and 100 µg/mL), semen samples from four rams were combined. Sperm motility, viability, DNA and plasma membrane integrity, total abnormalities and malondialdehyde (MDA) generation were all evaluated in treatment groups after thawing. Results The results showed that adding 1 µg/mL of ZnO‐NPs and Curc‐co‐ZnO‐NPs significantly reduced the level of MDA and total abnormalities (p < 0.05). Additionally, following the freeze‐thawing procedure, the presence of 1 µg/mL of Curc‐co‐ZnO‐NPs in the diluent of ram sperm significantly increased the percentage of sperm viability and motility in comparison to the control and other treatment groups (p < 0.05). Furthermore, as compared to the control group and other treatments, treatments containing 1 µg/mL of Curc‐co‐ZnO‐NPs significantly improved membrane and DNA integrity (p < 0.05). Conclusions It appears that following freeze‐thawing, the Curc‐co‐ZnO‐NPs (1 µg/mL) enhanced sperm parameters.https://doi.org/10.1002/vms3.70091cryopreservationCurc‐co‐ZnO‐NPscurcuminramZnO‐NPs |
| spellingShingle | Fatemeh Omidi Hadi Hajarian Hamed Karamishabankareh Leila Soltani Mojtaba Dashtizad Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram Semen Veterinary Medicine and Science cryopreservation Curc‐co‐ZnO‐NPs curcumin ram ZnO‐NPs |
| title | Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram Semen |
| title_full | Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram Semen |
| title_fullStr | Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram Semen |
| title_full_unstemmed | Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram Semen |
| title_short | Comparison of the Effect of Adding Different Levels of Zinc Chloride, Curcumin, Zinc Oxide Nanoparticles (Zano‐NPs), Curcumin Loaded on Zano‐NPs on Post‐Thawing Quality of Ram Semen |
| title_sort | comparison of the effect of adding different levels of zinc chloride curcumin zinc oxide nanoparticles zano nps curcumin loaded on zano nps on post thawing quality of ram semen |
| topic | cryopreservation Curc‐co‐ZnO‐NPs curcumin ram ZnO‐NPs |
| url | https://doi.org/10.1002/vms3.70091 |
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