Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis
Objective. To investigate the effects of regulatory B (Breg) cells and T helper 17 (Th17) cells on pathogenesis of ulcerative colitis, explore the clinical significance of Breg/Th17 ratio on the prognosis of ulcerative colitis, and provide the theoretical basis for the targeted therapy, diagnosis, a...
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Wiley
2018-01-01
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| Series: | Canadian Journal of Gastroenterology and Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2018/5760849 |
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| author | Xue Bing Liang Linlang Chen Keyan |
| author_facet | Xue Bing Liang Linlang Chen Keyan |
| author_sort | Xue Bing |
| collection | DOAJ |
| description | Objective. To investigate the effects of regulatory B (Breg) cells and T helper 17 (Th17) cells on pathogenesis of ulcerative colitis, explore the clinical significance of Breg/Th17 ratio on the prognosis of ulcerative colitis, and provide the theoretical basis for the targeted therapy, diagnosis, and prognosis of the disease. Methods. Peripheral blood and colonic mucosa were collected from patients with ulcerative colitis. Hematoxylin-eosin staining was used to observe the pathological changes of colonic mucosa. Flow cytometry was utilized to analyze the percentages of Breg cells and Th17 cells. Real-time fluorescent quantitative polymerase chain reaction and immunohistochemistry were applied to determine the expression of Breg cells-related cytokines IL-10 and Th17 cell transcription factor RORγT. Enzyme-linked immunosorbent assay was employed to detect serum IL-10 and IL-17 levels. Results. The colonic mucosa of ulcerative colitis patients presented massive inflammatory cell infiltration and hemorrhagic necrosis. The number of Breg cells and Th17 cells, the gene expressions of IL-10 and RORγT, and serum levels of IL-10 and IL-17 all increased in peripheral blood. Compared with nonremission group, the remission group showed that the percentage of Breg cells reduced, the percentage of Th17 cells increased, and thus the B10/Th17 ratio was significantly decreased in peripheral blood. In addition, serum IL-10 levels diminished, IL-17 levels increased, and thus IL-10/IL-17 ratio was remarkably reduced in remission group. B10/Th17 ratio and IL-10/IL-17 ratio were positively correlated with the severity of disease. Conclusions. Breg and Th17 cells participate in the occurrence and development of ulcerative colitis. B10/Th17 ratio and IL-10/IL-17 ratio can be used as prognostic markers for ulcerative colitis. This provides a theoretical basis for design of targeted treatment and prognosis assessment of the disease. |
| format | Article |
| id | doaj-art-1184f1c484ad412fbf35801803f24790 |
| institution | Kabale University |
| issn | 2291-2789 2291-2797 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology and Hepatology |
| spelling | doaj-art-1184f1c484ad412fbf35801803f247902025-02-03T01:03:12ZengWileyCanadian Journal of Gastroenterology and Hepatology2291-27892291-27972018-01-01201810.1155/2018/57608495760849Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative ColitisXue Bing0Liang Linlang1Chen Keyan2Department of Endocrinology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenyang, Liaoning Province 110016, ChinaDepartment of Endocrinology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenyang, Liaoning Province 110016, ChinaDepartment of Laboratory Animal Science, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province 110122, ChinaObjective. To investigate the effects of regulatory B (Breg) cells and T helper 17 (Th17) cells on pathogenesis of ulcerative colitis, explore the clinical significance of Breg/Th17 ratio on the prognosis of ulcerative colitis, and provide the theoretical basis for the targeted therapy, diagnosis, and prognosis of the disease. Methods. Peripheral blood and colonic mucosa were collected from patients with ulcerative colitis. Hematoxylin-eosin staining was used to observe the pathological changes of colonic mucosa. Flow cytometry was utilized to analyze the percentages of Breg cells and Th17 cells. Real-time fluorescent quantitative polymerase chain reaction and immunohistochemistry were applied to determine the expression of Breg cells-related cytokines IL-10 and Th17 cell transcription factor RORγT. Enzyme-linked immunosorbent assay was employed to detect serum IL-10 and IL-17 levels. Results. The colonic mucosa of ulcerative colitis patients presented massive inflammatory cell infiltration and hemorrhagic necrosis. The number of Breg cells and Th17 cells, the gene expressions of IL-10 and RORγT, and serum levels of IL-10 and IL-17 all increased in peripheral blood. Compared with nonremission group, the remission group showed that the percentage of Breg cells reduced, the percentage of Th17 cells increased, and thus the B10/Th17 ratio was significantly decreased in peripheral blood. In addition, serum IL-10 levels diminished, IL-17 levels increased, and thus IL-10/IL-17 ratio was remarkably reduced in remission group. B10/Th17 ratio and IL-10/IL-17 ratio were positively correlated with the severity of disease. Conclusions. Breg and Th17 cells participate in the occurrence and development of ulcerative colitis. B10/Th17 ratio and IL-10/IL-17 ratio can be used as prognostic markers for ulcerative colitis. This provides a theoretical basis for design of targeted treatment and prognosis assessment of the disease.http://dx.doi.org/10.1155/2018/5760849 |
| spellingShingle | Xue Bing Liang Linlang Chen Keyan Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis Canadian Journal of Gastroenterology and Hepatology |
| title | Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis |
| title_full | Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis |
| title_fullStr | Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis |
| title_full_unstemmed | Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis |
| title_short | Decreased Breg/Th17 Ratio Improved the Prognosis of Patients with Ulcerative Colitis |
| title_sort | decreased breg th17 ratio improved the prognosis of patients with ulcerative colitis |
| url | http://dx.doi.org/10.1155/2018/5760849 |
| work_keys_str_mv | AT xuebing decreasedbregth17ratioimprovedtheprognosisofpatientswithulcerativecolitis AT lianglinlang decreasedbregth17ratioimprovedtheprognosisofpatientswithulcerativecolitis AT chenkeyan decreasedbregth17ratioimprovedtheprognosisofpatientswithulcerativecolitis |