Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell Proliferation
<i>Phytomonas</i> is the only kinetoplastid that can parasitize plants, causing economically relevant issues. <i>Phytomonas serpens</i> share similarities with pathogenic trypanosomatids, including surface enzymes that are involved in adhesion to the salivary gland of their e...
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2024-12-01
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| Series: | Kinases and Phosphatases |
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| author | Luiz Fernando Carvalho-Kelly Anita Leocadio Freitas-Mesquita Thaís Souza Silveira Majerowicz José Roberto Meyer-Fernandes |
| author_facet | Luiz Fernando Carvalho-Kelly Anita Leocadio Freitas-Mesquita Thaís Souza Silveira Majerowicz José Roberto Meyer-Fernandes |
| author_sort | Luiz Fernando Carvalho-Kelly |
| collection | DOAJ |
| description | <i>Phytomonas</i> is the only kinetoplastid that can parasitize plants, causing economically relevant issues. <i>Phytomonas serpens</i> share similarities with pathogenic trypanosomatids, including surface enzymes that are involved in adhesion to the salivary gland of their experimental host, the insect <i>Oncopeltus fasciatus</i>. Ectophosphatases are cell surface enzymes involved in host–parasite interactions that are widely distributed among microorganisms. This work aimed to perform the biochemical characterization of <i>P. serpens</i> ectophosphatase activity, investigating and discussing its possible physiological role. This activity presented an acidic profile, and its kinetic parameters <i>K</i><sub>m</sub> and <i>V</i><sub>max</sub> were calculated as 1.57 ± 0.08 mM <i>p</i>-NPP and 10.11 ± 0.14 nmol <i>p</i>-NP/(h × 10<sup>8</sup> flagellates), respectively. It was stimulated by cobalt, inhibited by zinc, and insensitive to EDTA, a divalent metal chelator. The inhibitor sodium orthovanadate was able to decrease <i>P. serpens</i> ectophosphatase activity and growth, suggesting its involvement in cell proliferation. Given that <i>P. serpens</i> can uptake inorganic phosphate (P<sub>i</sub>) from the extracellular medium, it is likely that its ectophosphatase activity acts together with the transport systems in the P<sub>i</sub> acquisition process. The elucidation of the molecular mechanisms involved in this process emerges as a relevant perspective, providing new strategies for controlling <i>Phytomonas</i> infection. |
| format | Article |
| id | doaj-art-1182e64a1b1d44c190dc6444ffb65fb9 |
| institution | Kabale University |
| issn | 2813-3757 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Kinases and Phosphatases |
| spelling | doaj-art-1182e64a1b1d44c190dc6444ffb65fb92025-01-24T13:37:30ZengMDPI AGKinases and Phosphatases2813-37572024-12-012437939010.3390/kinasesphosphatases2040024Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell ProliferationLuiz Fernando Carvalho-Kelly0Anita Leocadio Freitas-Mesquita1Thaís Souza Silveira Majerowicz2José Roberto Meyer-Fernandes3Instituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, RJ, BrazilInstituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, RJ, BrazilInstituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rua Senador Furtado, 121. Maracanã, Rio de Janeiro 20270-021, RJ, BrazilInstituto de Bioquímica Médica Leopoldo de Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil<i>Phytomonas</i> is the only kinetoplastid that can parasitize plants, causing economically relevant issues. <i>Phytomonas serpens</i> share similarities with pathogenic trypanosomatids, including surface enzymes that are involved in adhesion to the salivary gland of their experimental host, the insect <i>Oncopeltus fasciatus</i>. Ectophosphatases are cell surface enzymes involved in host–parasite interactions that are widely distributed among microorganisms. This work aimed to perform the biochemical characterization of <i>P. serpens</i> ectophosphatase activity, investigating and discussing its possible physiological role. This activity presented an acidic profile, and its kinetic parameters <i>K</i><sub>m</sub> and <i>V</i><sub>max</sub> were calculated as 1.57 ± 0.08 mM <i>p</i>-NPP and 10.11 ± 0.14 nmol <i>p</i>-NP/(h × 10<sup>8</sup> flagellates), respectively. It was stimulated by cobalt, inhibited by zinc, and insensitive to EDTA, a divalent metal chelator. The inhibitor sodium orthovanadate was able to decrease <i>P. serpens</i> ectophosphatase activity and growth, suggesting its involvement in cell proliferation. Given that <i>P. serpens</i> can uptake inorganic phosphate (P<sub>i</sub>) from the extracellular medium, it is likely that its ectophosphatase activity acts together with the transport systems in the P<sub>i</sub> acquisition process. The elucidation of the molecular mechanisms involved in this process emerges as a relevant perspective, providing new strategies for controlling <i>Phytomonas</i> infection.https://www.mdpi.com/2813-3757/2/4/24<i>Phytomonas serpens</i>ectophosphataseinorganic phosphatephosphate acquisition |
| spellingShingle | Luiz Fernando Carvalho-Kelly Anita Leocadio Freitas-Mesquita Thaís Souza Silveira Majerowicz José Roberto Meyer-Fernandes Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell Proliferation Kinases and Phosphatases <i>Phytomonas serpens</i> ectophosphatase inorganic phosphate phosphate acquisition |
| title | Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell Proliferation |
| title_full | Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell Proliferation |
| title_fullStr | Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell Proliferation |
| title_full_unstemmed | Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell Proliferation |
| title_short | Biochemical Properties of the Acid Ectophosphatase Activity of <i>Phytomonas serpens</i> Involved in Cell Proliferation |
| title_sort | biochemical properties of the acid ectophosphatase activity of i phytomonas serpens i involved in cell proliferation |
| topic | <i>Phytomonas serpens</i> ectophosphatase inorganic phosphate phosphate acquisition |
| url | https://www.mdpi.com/2813-3757/2/4/24 |
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