Cost-effective whole-cell biosynthesis of ursodeoxycholic acid using engineered Escherichia coli with a multienzyme cascade

Cost-effective whole-cell biosynthesis of ursodeoxycholic acid using engineered Escherichia coli with a multienzyme cascade

Ursodeoxycholic acid (UDCA) can be used as a drug to treat various liver and bile diseases. Currently, the biological synthesis of UDCA is predominantly conducted via a two-step enzymatic process in which synthesis is catalyzed by 7α-hydroxysteroid dehydrogenase (7α-HSDH) and 7β-hydroxysteroid dehyd...

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Bibliographic Details
Main Authors:	Xue Zhang, Jiagang Xin, Mengyu Liu, Yue Zhang, Haoni Luan, Wei Feng, Fei Wang, Wei Xu, Peng Song
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-01-01
Series:	Frontiers in Microbiology
Subjects:	chenodeoxycholic acid ursodeoxycholic acid whole-cell catalysis 7α-hydroxysteroid dehydrogenase 7β-hydroxysteroid dehydrogenase lactate dehydrogenase
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2025.1538237/full
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