A Study on L-Asparaginase of Nocardia levis MK-VL_113
An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in m...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/160434 |
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| _version_ | 1832566734057373696 |
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| author | Alapati Kavitha Muvva Vijayalakshmi |
| author_facet | Alapati Kavitha Muvva Vijayalakshmi |
| author_sort | Alapati Kavitha |
| collection | DOAJ |
| description | An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 30∘C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase by N. levis. |
| format | Article |
| id | doaj-art-1174ea793c1749f89506644d81f3496b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-1174ea793c1749f89506644d81f3496b2025-02-03T01:03:15ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/160434160434A Study on L-Asparaginase of Nocardia levis MK-VL_113Alapati Kavitha0Muvva Vijayalakshmi1Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522 510, IndiaDepartment of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522 510, IndiaAn enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 30∘C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase by N. levis.http://dx.doi.org/10.1100/2012/160434 |
| spellingShingle | Alapati Kavitha Muvva Vijayalakshmi A Study on L-Asparaginase of Nocardia levis MK-VL_113 The Scientific World Journal |
| title | A Study on L-Asparaginase of Nocardia levis MK-VL_113 |
| title_full | A Study on L-Asparaginase of Nocardia levis MK-VL_113 |
| title_fullStr | A Study on L-Asparaginase of Nocardia levis MK-VL_113 |
| title_full_unstemmed | A Study on L-Asparaginase of Nocardia levis MK-VL_113 |
| title_short | A Study on L-Asparaginase of Nocardia levis MK-VL_113 |
| title_sort | study on l asparaginase of nocardia levis mk vl 113 |
| url | http://dx.doi.org/10.1100/2012/160434 |
| work_keys_str_mv | AT alapatikavitha astudyonlasparaginaseofnocardialevismkvl113 AT muvvavijayalakshmi astudyonlasparaginaseofnocardialevismkvl113 AT alapatikavitha studyonlasparaginaseofnocardialevismkvl113 AT muvvavijayalakshmi studyonlasparaginaseofnocardialevismkvl113 |