Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma
A LC-MS/MS method for determination of stavudine in human plasma was established and validated, and it was applied to the pharmaceutical formulations bioequivalence study. 0.5 mL plasma sample was extracted by liquid-liquid extraction. Stavudine was detected by a LC-MS/MS system. The pharmacokinetic...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2014/517089 |
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| author | Fengdan Jin |
| author_facet | Fengdan Jin |
| author_sort | Fengdan Jin |
| collection | DOAJ |
| description | A LC-MS/MS method for determination of stavudine in human plasma was established and validated, and it was applied to the pharmaceutical formulations bioequivalence study. 0.5 mL plasma sample was extracted by liquid-liquid extraction. Stavudine was detected by a LC-MS/MS system. The pharmacokinetic parameters of stavudine in different formulations were calculated by noncompartment model statistics. The method was linear over the concentration ranges 5.00–1000 ng/mL in plasma. The intra- and interassay relative standard deviation (RSD) was <10%. The average accuracies for the assay at three concentrations (5.00, 80.0, and 900 ng/mL) were from 100.2% to 102.5%. Pharmacokinetic parameters of stavudine reference formulation were obtained as follows: Tmax was 0.6±0.2 h, Cmax was 480.7±150.9 g/L, t1/2 was 1.7±0.4 h, and AUC0-t was 872.8±227.8 g·h/L, and pharmacokinetic parameters of stavudine test formulation were obtained as follows: Tmax was 0.5±0.2 h, Cmax was 537.5±178.5 g/L, t1/2 was 1.7±0.3 h, and AUC0-t was (914.1±284.5) g·h/L. Calculated with AUC0-t, the bioavailability of two formulations was 105.0%. |
| format | Article |
| id | doaj-art-11670bdeb65e4320a44e37b6d35cc152 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-11670bdeb65e4320a44e37b6d35cc1522025-02-03T06:01:41ZengWileyJournal of Spectroscopy2314-49202314-49392014-01-01201410.1155/2014/517089517089Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human PlasmaFengdan Jin0Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao, Hebei 066004, ChinaA LC-MS/MS method for determination of stavudine in human plasma was established and validated, and it was applied to the pharmaceutical formulations bioequivalence study. 0.5 mL plasma sample was extracted by liquid-liquid extraction. Stavudine was detected by a LC-MS/MS system. The pharmacokinetic parameters of stavudine in different formulations were calculated by noncompartment model statistics. The method was linear over the concentration ranges 5.00–1000 ng/mL in plasma. The intra- and interassay relative standard deviation (RSD) was <10%. The average accuracies for the assay at three concentrations (5.00, 80.0, and 900 ng/mL) were from 100.2% to 102.5%. Pharmacokinetic parameters of stavudine reference formulation were obtained as follows: Tmax was 0.6±0.2 h, Cmax was 480.7±150.9 g/L, t1/2 was 1.7±0.4 h, and AUC0-t was 872.8±227.8 g·h/L, and pharmacokinetic parameters of stavudine test formulation were obtained as follows: Tmax was 0.5±0.2 h, Cmax was 537.5±178.5 g/L, t1/2 was 1.7±0.3 h, and AUC0-t was (914.1±284.5) g·h/L. Calculated with AUC0-t, the bioavailability of two formulations was 105.0%.http://dx.doi.org/10.1155/2014/517089 |
| spellingShingle | Fengdan Jin Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma Journal of Spectroscopy |
| title | Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma |
| title_full | Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma |
| title_fullStr | Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma |
| title_full_unstemmed | Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma |
| title_short | Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma |
| title_sort | liquid chromatography tandem mass spectrometric assay for determination of stavudine in human plasma |
| url | http://dx.doi.org/10.1155/2014/517089 |
| work_keys_str_mv | AT fengdanjin liquidchromatographytandemmassspectrometricassayfordeterminationofstavudineinhumanplasma |