Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata
Lemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences. Several species of this family represent the smallest and fastest growing flowering plants. Different ecotypes of the same species vary in their biochemical and physiological properties. Thus,...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/5196763 |
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| author | Bo Feng Yang Fang Zhibin Xu Chao Xiang Chunhong Zhou Fei Jiang Tao Wang Hai Zhao |
| author_facet | Bo Feng Yang Fang Zhibin Xu Chao Xiang Chunhong Zhou Fei Jiang Tao Wang Hai Zhao |
| author_sort | Bo Feng |
| collection | DOAJ |
| description | Lemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences. Several species of this family represent the smallest and fastest growing flowering plants. Different ecotypes of the same species vary in their biochemical and physiological properties. Thus, selecting of desirable ecotypes of a species is very important. Here, we developed a simple and rapid molecular identification system for Spirodela polyrhiza and Landoltia punctata based on the sequence polymorphism. First, several pairs of primers were designed and three markers were selected as good for identification. After PCR amplification, DNA fragments (the combination of three PCR products) in different duckweeds were detected using capillary electrophoresis. The high-resolution capillary electrophoresis displayed high identity to the sequencing results. The combination of the PCR products containing several DNA fragments highly improved the identification frequency. These results indicate that this method is not only good for interspecies identification but also ideal for intraspecies distinguishing. Meanwhile, 11 haplotypes were found in both the S. polyrhiza and L. punctata ecotypes. The results suggest that this marker system is useful for large-scale identification of duckweed and for the screening of desirable ecotypes to improve the diverse usage in duckweed utilization. |
| format | Article |
| id | doaj-art-112f6ee94ca943848f74505b78c3b3b2 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-112f6ee94ca943848f74505b78c3b3b22025-02-03T06:11:39ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/51967635196763Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctataBo Feng0Yang Fang1Zhibin Xu2Chao Xiang3Chunhong Zhou4Fei Jiang5Tao Wang6Hai Zhao7Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaChengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaLemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences. Several species of this family represent the smallest and fastest growing flowering plants. Different ecotypes of the same species vary in their biochemical and physiological properties. Thus, selecting of desirable ecotypes of a species is very important. Here, we developed a simple and rapid molecular identification system for Spirodela polyrhiza and Landoltia punctata based on the sequence polymorphism. First, several pairs of primers were designed and three markers were selected as good for identification. After PCR amplification, DNA fragments (the combination of three PCR products) in different duckweeds were detected using capillary electrophoresis. The high-resolution capillary electrophoresis displayed high identity to the sequencing results. The combination of the PCR products containing several DNA fragments highly improved the identification frequency. These results indicate that this method is not only good for interspecies identification but also ideal for intraspecies distinguishing. Meanwhile, 11 haplotypes were found in both the S. polyrhiza and L. punctata ecotypes. The results suggest that this marker system is useful for large-scale identification of duckweed and for the screening of desirable ecotypes to improve the diverse usage in duckweed utilization.http://dx.doi.org/10.1155/2017/5196763 |
| spellingShingle | Bo Feng Yang Fang Zhibin Xu Chao Xiang Chunhong Zhou Fei Jiang Tao Wang Hai Zhao Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata International Journal of Genomics |
| title | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
| title_full | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
| title_fullStr | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
| title_full_unstemmed | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
| title_short | Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata |
| title_sort | development of a new marker system for identification of spirodela polyrhiza and landoltia punctata |
| url | http://dx.doi.org/10.1155/2017/5196763 |
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