Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa
Secretory leukocyte protease inhibitor (SLPI) is a wellknown protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350120093740 |
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| _version_ | 1832559811268444160 |
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| author | Max Nyström Ulla Peterson Westin Carina Linder Kjell Ohlsson |
| author_facet | Max Nyström Ulla Peterson Westin Carina Linder Kjell Ohlsson |
| author_sort | Max Nyström |
| collection | DOAJ |
| description | Secretory leukocyte protease inhibitor (SLPI) is a wellknown protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather
than on the area of the biopsy. |
| format | Article |
| id | doaj-art-1128fc2ef89e4d3db1bac0a74beb2a13 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1128fc2ef89e4d3db1bac0a74beb2a132025-02-03T01:29:08ZengWileyMediators of Inflammation0962-93511466-18612001-01-0110526927210.1080/09629350120093740Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosaMax Nyström0Ulla Peterson Westin1Carina Linder2Kjell Ohlsson3Department of Surgery, Ing 37, Lund University, University Hospital Malmö, Malmö SE 20502, SwedenDepartment of Surgical Pathophysiology, Ing 37, Lund University, University Hospital Malmö, Malmö SE 20502, SwedenDepartment of Surgical Pathophysiology, Ing 37, Lund University, University Hospital Malmö, Malmö SE 20502, SwedenDepartment of Surgical Pathophysiology, Ing 37, Lund University, University Hospital Malmö, Malmö SE 20502, SwedenSecretory leukocyte protease inhibitor (SLPI) is a wellknown protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.http://dx.doi.org/10.1080/09629350120093740 |
| spellingShingle | Max Nyström Ulla Peterson Westin Carina Linder Kjell Ohlsson Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa Mediators of Inflammation |
| title | Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa |
| title_full | Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa |
| title_fullStr | Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa |
| title_full_unstemmed | Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa |
| title_short | Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa |
| title_sort | secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa |
| url | http://dx.doi.org/10.1080/09629350120093740 |
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