Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale
Rhein is an important quality-control marker of Rheum officinale. The aim of this study was to develop an indirect competitive enzyme-linked immunosorbent assay (icELISA) for rhein detection, which acts as a powerful tool for quality control and proper usage of Rheum officinale. First, a specific an...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/4294826 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550330299056128 |
|---|---|
| author | Min Chen Tie-Gui Nan Jie Xin Li Cui Bo Zhang Xiao Wang Bao-Min Wang |
| author_facet | Min Chen Tie-Gui Nan Jie Xin Li Cui Bo Zhang Xiao Wang Bao-Min Wang |
| author_sort | Min Chen |
| collection | DOAJ |
| description | Rhein is an important quality-control marker of Rheum officinale. The aim of this study was to develop an indirect competitive enzyme-linked immunosorbent assay (icELISA) for rhein detection, which acts as a powerful tool for quality control and proper usage of Rheum officinale. First, a specific and sensitive monoclonal antibody (mAb) against rhein was produced from a stable hybridoma cell line, 1F8, generated by the fusion of mouse myeloma sp2/0 with spleen cells obtained from a Bal b/c mouse immunized with rhein-BSA. Then, an icELISA method was developed with an IC50 value and working range of 0.05 μg L−1 and 0.02–0.11 μg L−1, respectively. The icELISA revealed high assay specificity, since it only had a relatively high cross reactivity with aloe-emodin (27%) and almost no cross reactivity with any other anthraquinones (<1%). When spiked with 0.2–2 mg kg−1 of rhein, the recoveries ranged from 84.19% to 102.90%. Finally, icELISA was used to detect rhein contents of Rheum officinale collected from different regions, and the results corresponded well with those of HPLC. Overall, the developed icELISA with high specificity and sensitivity provided a rapid and simple method for rhein detection, and it may be a powerful tool for quality control and proper usage of Rheum officinale. |
| format | Article |
| id | doaj-art-10f72aeb2c204ff4bb7b6464b9e3d9b2 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-10f72aeb2c204ff4bb7b6464b9e3d9b22025-02-03T06:06:55ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792020-01-01202010.1155/2020/42948264294826Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinaleMin Chen0Tie-Gui Nan1Jie Xin2Li Cui3Bo Zhang4Xiao Wang5Bao-Min Wang6School of Pharmacy, Linyi University, Linyi 276000, ChinaState Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaSchool of Pharmacy, Linyi University, Linyi 276000, ChinaKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaSchool of Pharmacy, Linyi University, Linyi 276000, ChinaKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaCollege of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaRhein is an important quality-control marker of Rheum officinale. The aim of this study was to develop an indirect competitive enzyme-linked immunosorbent assay (icELISA) for rhein detection, which acts as a powerful tool for quality control and proper usage of Rheum officinale. First, a specific and sensitive monoclonal antibody (mAb) against rhein was produced from a stable hybridoma cell line, 1F8, generated by the fusion of mouse myeloma sp2/0 with spleen cells obtained from a Bal b/c mouse immunized with rhein-BSA. Then, an icELISA method was developed with an IC50 value and working range of 0.05 μg L−1 and 0.02–0.11 μg L−1, respectively. The icELISA revealed high assay specificity, since it only had a relatively high cross reactivity with aloe-emodin (27%) and almost no cross reactivity with any other anthraquinones (<1%). When spiked with 0.2–2 mg kg−1 of rhein, the recoveries ranged from 84.19% to 102.90%. Finally, icELISA was used to detect rhein contents of Rheum officinale collected from different regions, and the results corresponded well with those of HPLC. Overall, the developed icELISA with high specificity and sensitivity provided a rapid and simple method for rhein detection, and it may be a powerful tool for quality control and proper usage of Rheum officinale.http://dx.doi.org/10.1155/2020/4294826 |
| spellingShingle | Min Chen Tie-Gui Nan Jie Xin Li Cui Bo Zhang Xiao Wang Bao-Min Wang Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale International Journal of Analytical Chemistry |
| title | Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale |
| title_full | Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale |
| title_fullStr | Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale |
| title_full_unstemmed | Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale |
| title_short | Development of an Enzyme-Linked Immunosorbent Assay Method for the Detection of Rhein in Rheum officinale |
| title_sort | development of an enzyme linked immunosorbent assay method for the detection of rhein in rheum officinale |
| url | http://dx.doi.org/10.1155/2020/4294826 |
| work_keys_str_mv | AT minchen developmentofanenzymelinkedimmunosorbentassaymethodforthedetectionofrheininrheumofficinale AT tieguinan developmentofanenzymelinkedimmunosorbentassaymethodforthedetectionofrheininrheumofficinale AT jiexin developmentofanenzymelinkedimmunosorbentassaymethodforthedetectionofrheininrheumofficinale AT licui developmentofanenzymelinkedimmunosorbentassaymethodforthedetectionofrheininrheumofficinale AT bozhang developmentofanenzymelinkedimmunosorbentassaymethodforthedetectionofrheininrheumofficinale AT xiaowang developmentofanenzymelinkedimmunosorbentassaymethodforthedetectionofrheininrheumofficinale AT baominwang developmentofanenzymelinkedimmunosorbentassaymethodforthedetectionofrheininrheumofficinale |