Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis
Granulocyte colony-stimulating factor (G-CSF) can promote the repair of a variety of damaged tissues, but the underlying mechanisms have not yet been fully elucidated. Bone marrow mesenchymal stem cells (BM-MSCs) play an important role in the repair of damaged tissue. The aim of this study was to ex...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/1726743 |
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| author | Feiyan Zhao Wei Liu Shaojie Yue Lei Yang Qingzhong Hua Yan Zhou Haipeng Cheng Ziqiang Luo Siyuan Tang |
| author_facet | Feiyan Zhao Wei Liu Shaojie Yue Lei Yang Qingzhong Hua Yan Zhou Haipeng Cheng Ziqiang Luo Siyuan Tang |
| author_sort | Feiyan Zhao |
| collection | DOAJ |
| description | Granulocyte colony-stimulating factor (G-CSF) can promote the repair of a variety of damaged tissues, but the underlying mechanisms have not yet been fully elucidated. Bone marrow mesenchymal stem cells (BM-MSCs) play an important role in the repair of damaged tissue. The aim of this study was to explore whether pretreating BM-MSCs with G-CSF can promote their ability of homing to the lung after in vitro transplantation via upregulating the CXCR4 expression, potentially markedly increasing the antifibrotic effect of BM-MSCs. The BM-MSCs pretreated with G-CSF were transplanted into a mouse on day 14 after bleomycin injection. The antifibrotic effects of BM-MSCs in mice were tested on day 21 by using pathological examination and collagen content assay. Pretreatment of BM-MSCs with G-CSF significantly promoted their ability of homing to the lung and enhanced their antifibrotic effects. However, knocking down the CXCR4 expression in BM-MSCs significantly inhibited the ability of G-CSF to promote the migration and homing of BM-MSCs to the lung and the resulting antifibrotic effects. We also found that G-CSF significantly increased the CXCR4 expression and AKT phosphorylation in BM-MSCs, and the AKT pathway inhibitor LY294002 significantly diminished the ability of G-CSF to upregulate the CXCR4 expression in BM-MSCs. Pretreatment of BM-MSCs with G-CSF promotes the homing of BM-MSCs to the lung via upregulating the CXCR4 expression, leading to a marked increase in the antifibrotic effects of BM-MSCs. This study provides new avenues for the application of BM-MSCs in the repair of different tissues. |
| format | Article |
| id | doaj-art-10f1ad17b3144c16a44fe7c5c94e91dc |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-10f1ad17b3144c16a44fe7c5c94e91dc2025-02-03T01:00:00ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/17267431726743Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary FibrosisFeiyan Zhao0Wei Liu1Shaojie Yue2Lei Yang3Qingzhong Hua4Yan Zhou5Haipeng Cheng6Ziqiang Luo7Siyuan Tang8Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, ChinaXiangya Nursing School, Central South University, Changsha, Hunan, ChinaDepartment of Pediatrics, Xiangya Hospital, Central South University, Changsha, Hunan, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, ChinaXiangya Nursing School, Central South University, Changsha, Hunan, ChinaDepartment of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, ChinaDepartment of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, ChinaDepartment of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, ChinaXiangya Nursing School, Central South University, Changsha, Hunan, ChinaGranulocyte colony-stimulating factor (G-CSF) can promote the repair of a variety of damaged tissues, but the underlying mechanisms have not yet been fully elucidated. Bone marrow mesenchymal stem cells (BM-MSCs) play an important role in the repair of damaged tissue. The aim of this study was to explore whether pretreating BM-MSCs with G-CSF can promote their ability of homing to the lung after in vitro transplantation via upregulating the CXCR4 expression, potentially markedly increasing the antifibrotic effect of BM-MSCs. The BM-MSCs pretreated with G-CSF were transplanted into a mouse on day 14 after bleomycin injection. The antifibrotic effects of BM-MSCs in mice were tested on day 21 by using pathological examination and collagen content assay. Pretreatment of BM-MSCs with G-CSF significantly promoted their ability of homing to the lung and enhanced their antifibrotic effects. However, knocking down the CXCR4 expression in BM-MSCs significantly inhibited the ability of G-CSF to promote the migration and homing of BM-MSCs to the lung and the resulting antifibrotic effects. We also found that G-CSF significantly increased the CXCR4 expression and AKT phosphorylation in BM-MSCs, and the AKT pathway inhibitor LY294002 significantly diminished the ability of G-CSF to upregulate the CXCR4 expression in BM-MSCs. Pretreatment of BM-MSCs with G-CSF promotes the homing of BM-MSCs to the lung via upregulating the CXCR4 expression, leading to a marked increase in the antifibrotic effects of BM-MSCs. This study provides new avenues for the application of BM-MSCs in the repair of different tissues.http://dx.doi.org/10.1155/2019/1726743 |
| spellingShingle | Feiyan Zhao Wei Liu Shaojie Yue Lei Yang Qingzhong Hua Yan Zhou Haipeng Cheng Ziqiang Luo Siyuan Tang Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis Stem Cells International |
| title | Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis |
| title_full | Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis |
| title_fullStr | Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis |
| title_full_unstemmed | Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis |
| title_short | Pretreatment with G-CSF Could Enhance the Antifibrotic Effect of BM-MSCs on Pulmonary Fibrosis |
| title_sort | pretreatment with g csf could enhance the antifibrotic effect of bm mscs on pulmonary fibrosis |
| url | http://dx.doi.org/10.1155/2019/1726743 |
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