Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet Regime
The diaphragm is a dome-shaped skeletal muscle indispensable for breathing. Its activity contributes up to 70% of the total ventilatory function at rest. In comparison to other skeletal muscles, it is distinguished by an oxidative phenotype and uninterrupted cyclic contraction pattern. Surprisingly,...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/3437169 |
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| author | Bartlomiej Lukaszuk Agnieszka Miklosz Malgorzata Zendzian-Piotrowska Beata Wojcik Jan Gorski Adrian Chabowski |
| author_facet | Bartlomiej Lukaszuk Agnieszka Miklosz Malgorzata Zendzian-Piotrowska Beata Wojcik Jan Gorski Adrian Chabowski |
| author_sort | Bartlomiej Lukaszuk |
| collection | DOAJ |
| description | The diaphragm is a dome-shaped skeletal muscle indispensable for breathing. Its activity contributes up to 70% of the total ventilatory function at rest. In comparison to other skeletal muscles, it is distinguished by an oxidative phenotype and uninterrupted cyclic contraction pattern. Surprisingly, the research regarding diaphragm diabetic phenotype particularly in the light of lipid-induced insulin resistance is virtually nonexistent. Male Wistar rats were randomly allocated into 3 groups: control, streptozotocin-induced (STZ) type-1 diabetes, and rodents fed with high-fat diet (HFD). Additionally, half of the animals from each group were administered with myriocin, a robust, selective inhibitor of ceramide synthesis and, therefore, a potent agent ameliorating insulin resistance. Diaphragm lipid contents were evaluated using chromatography. Fatty acid transporter expression was determined by Western blot. The STZ and HFD rats had increased concentration of lipids, namely, ceramides (CER) and diacylglycerols (DAG). Interestingly, this coincided with an increased concentration of long-chain (C ≥ 16) saturated fatty acid species present in both the aforementioned lipid fractions. The CER/DAG accumulation was accompanied by an elevated fatty acid transporter expression (FATP-1 in HFD and FATP-4 in STZ). Surprisingly, we observed a significantly decreased triacylglycerol content in the diaphragms of STZ-treated rats. |
| format | Article |
| id | doaj-art-10e157d4f2b043b3bef10903017a1dcb |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-10e157d4f2b043b3bef10903017a1dcb2025-02-03T01:13:05ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/34371693437169Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet RegimeBartlomiej Lukaszuk0Agnieszka Miklosz1Malgorzata Zendzian-Piotrowska2Beata Wojcik3Jan Gorski4Adrian Chabowski5Department of Physiology, Medical University of Bialystok, Bialystok, PolandDepartment of Physiology, Medical University of Bialystok, Bialystok, PolandDepartment of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Bialystok, PolandDepartment of Physiology, Medical University of Bialystok, Bialystok, PolandDepartment of Physiology, Medical University of Bialystok, Bialystok, PolandDepartment of Physiology, Medical University of Bialystok, Bialystok, PolandThe diaphragm is a dome-shaped skeletal muscle indispensable for breathing. Its activity contributes up to 70% of the total ventilatory function at rest. In comparison to other skeletal muscles, it is distinguished by an oxidative phenotype and uninterrupted cyclic contraction pattern. Surprisingly, the research regarding diaphragm diabetic phenotype particularly in the light of lipid-induced insulin resistance is virtually nonexistent. Male Wistar rats were randomly allocated into 3 groups: control, streptozotocin-induced (STZ) type-1 diabetes, and rodents fed with high-fat diet (HFD). Additionally, half of the animals from each group were administered with myriocin, a robust, selective inhibitor of ceramide synthesis and, therefore, a potent agent ameliorating insulin resistance. Diaphragm lipid contents were evaluated using chromatography. Fatty acid transporter expression was determined by Western blot. The STZ and HFD rats had increased concentration of lipids, namely, ceramides (CER) and diacylglycerols (DAG). Interestingly, this coincided with an increased concentration of long-chain (C ≥ 16) saturated fatty acid species present in both the aforementioned lipid fractions. The CER/DAG accumulation was accompanied by an elevated fatty acid transporter expression (FATP-1 in HFD and FATP-4 in STZ). Surprisingly, we observed a significantly decreased triacylglycerol content in the diaphragms of STZ-treated rats.http://dx.doi.org/10.1155/2017/3437169 |
| spellingShingle | Bartlomiej Lukaszuk Agnieszka Miklosz Malgorzata Zendzian-Piotrowska Beata Wojcik Jan Gorski Adrian Chabowski Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet Regime Journal of Diabetes Research |
| title | Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet Regime |
| title_full | Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet Regime |
| title_fullStr | Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet Regime |
| title_full_unstemmed | Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet Regime |
| title_short | Changes in the Diaphragm Lipid Content after Administration of Streptozotocin and High-Fat Diet Regime |
| title_sort | changes in the diaphragm lipid content after administration of streptozotocin and high fat diet regime |
| url | http://dx.doi.org/10.1155/2017/3437169 |
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