Applying a cytochrome c oxidase I barcode for Leishmania species typing.
Species delimitation has always been a challenge for taxonomists and for Leishmania studies there is no exception. Herein we attempt to display the usefulness of the mitochondrial gene Cytochrome Oxidase I-coI in classical and barcode-based approaches for Leishmania characterization. A total of 228...
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Public Library of Science (PLoS)
2024-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0309277 |
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| author | Carlos Mata-Somarribas Grazielle Cardoso das Graças Luiza de Oliveira R Pereira Mariana Côrtes Boité Lilian Motta Cantanhêde Camila Patrício Braga Filgueira Adrián Fallas Leana Quirós-Rojas Karina A Morelli Gabriel Eduardo Melim Ferreira Elisa Cupolillo |
| author_facet | Carlos Mata-Somarribas Grazielle Cardoso das Graças Luiza de Oliveira R Pereira Mariana Côrtes Boité Lilian Motta Cantanhêde Camila Patrício Braga Filgueira Adrián Fallas Leana Quirós-Rojas Karina A Morelli Gabriel Eduardo Melim Ferreira Elisa Cupolillo |
| author_sort | Carlos Mata-Somarribas |
| collection | DOAJ |
| description | Species delimitation has always been a challenge for taxonomists and for Leishmania studies there is no exception. Herein we attempt to display the usefulness of the mitochondrial gene Cytochrome Oxidase I-coI in classical and barcode-based approaches for Leishmania characterization. A total of 228 samples were analyzed, comprising 28 Leishmania related taxa, mainly from cultures of the Oswaldo Cruz Foundation`s Leishmania Collection. Primers were designed for amplification of coI; sequences were analyzed by distance-based indicators and both the Neighbor Joining and NeighborNet as species grouping techniques. Automatic Barcode Gap Discovery was applied to define species delimitation while for the character-based analysis a software for Barcoding with Logic formulas was employed. Final sequences of 486 bp with 238 parsimonious sites were aligned and edited. Robust groups were formed for most of the genus species, distinctive nucleotide positions in the barcode sequence were observed for 11 of them. A good agreement between the techniques applied and the original characterization was observed. Few species were not distinguished by coI: (i) L. (V.) peruviana, L. (V.) lindenbergi, and L. (V.) utingensis; (ii) L. (L.) venezuelensis and (iii) L. colombiensis and L. equatorensis with identical sequences. Some of these taxa have been, at one time or another, classified as controversial and, for most of them, a higher number of isolates should be studied to properly infer their taxonomic status. CoI represents a mitochondrial target that stands out as a taxonomically important asset with multiple advantages over other genes. This paper corresponds to the first report of coI analysis in Leishmania, a potentially advantageous target for the characterization of this parasite. |
| format | Article |
| id | doaj-art-10de4f071d8046c6943be2b1c2d8fd9b |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-10de4f071d8046c6943be2b1c2d8fd9b2025-08-20T02:31:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e030927710.1371/journal.pone.0309277Applying a cytochrome c oxidase I barcode for Leishmania species typing.Carlos Mata-SomarribasGrazielle Cardoso das GraçasLuiza de Oliveira R PereiraMariana Côrtes BoitéLilian Motta CantanhêdeCamila Patrício Braga FilgueiraAdrián FallasLeana Quirós-RojasKarina A MorelliGabriel Eduardo Melim FerreiraElisa CupolilloSpecies delimitation has always been a challenge for taxonomists and for Leishmania studies there is no exception. Herein we attempt to display the usefulness of the mitochondrial gene Cytochrome Oxidase I-coI in classical and barcode-based approaches for Leishmania characterization. A total of 228 samples were analyzed, comprising 28 Leishmania related taxa, mainly from cultures of the Oswaldo Cruz Foundation`s Leishmania Collection. Primers were designed for amplification of coI; sequences were analyzed by distance-based indicators and both the Neighbor Joining and NeighborNet as species grouping techniques. Automatic Barcode Gap Discovery was applied to define species delimitation while for the character-based analysis a software for Barcoding with Logic formulas was employed. Final sequences of 486 bp with 238 parsimonious sites were aligned and edited. Robust groups were formed for most of the genus species, distinctive nucleotide positions in the barcode sequence were observed for 11 of them. A good agreement between the techniques applied and the original characterization was observed. Few species were not distinguished by coI: (i) L. (V.) peruviana, L. (V.) lindenbergi, and L. (V.) utingensis; (ii) L. (L.) venezuelensis and (iii) L. colombiensis and L. equatorensis with identical sequences. Some of these taxa have been, at one time or another, classified as controversial and, for most of them, a higher number of isolates should be studied to properly infer their taxonomic status. CoI represents a mitochondrial target that stands out as a taxonomically important asset with multiple advantages over other genes. This paper corresponds to the first report of coI analysis in Leishmania, a potentially advantageous target for the characterization of this parasite.https://doi.org/10.1371/journal.pone.0309277 |
| spellingShingle | Carlos Mata-Somarribas Grazielle Cardoso das Graças Luiza de Oliveira R Pereira Mariana Côrtes Boité Lilian Motta Cantanhêde Camila Patrício Braga Filgueira Adrián Fallas Leana Quirós-Rojas Karina A Morelli Gabriel Eduardo Melim Ferreira Elisa Cupolillo Applying a cytochrome c oxidase I barcode for Leishmania species typing. PLoS ONE |
| title | Applying a cytochrome c oxidase I barcode for Leishmania species typing. |
| title_full | Applying a cytochrome c oxidase I barcode for Leishmania species typing. |
| title_fullStr | Applying a cytochrome c oxidase I barcode for Leishmania species typing. |
| title_full_unstemmed | Applying a cytochrome c oxidase I barcode for Leishmania species typing. |
| title_short | Applying a cytochrome c oxidase I barcode for Leishmania species typing. |
| title_sort | applying a cytochrome c oxidase i barcode for leishmania species typing |
| url | https://doi.org/10.1371/journal.pone.0309277 |
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