Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells
Nitric oxide (NO), produced by endothelial nitric oxide synthase (eNOS), is a major antiatherogenic factor in the blood vessel. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases, including atherosclerosis. Decreased availability of endothelial NO promote...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/169370 |
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| author | Azizah Ugusman Zaiton Zakaria Kien Hui Chua Nor Anita Megat Mohd Nordin Zaleha Abdullah Mahdy |
| author_facet | Azizah Ugusman Zaiton Zakaria Kien Hui Chua Nor Anita Megat Mohd Nordin Zaleha Abdullah Mahdy |
| author_sort | Azizah Ugusman |
| collection | DOAJ |
| description | Nitric oxide (NO), produced by endothelial nitric oxide synthase (eNOS), is a major antiatherogenic factor in the blood vessel. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases, including atherosclerosis. Decreased availability of endothelial NO promotes the progression of endothelial dysfunction and atherosclerosis. Rutin is a flavonoid with multiple cardiovascular protective effects. This study aimed to investigate the effects of rutin on eNOS and NO production in cultured human umbilical vein endothelial cells (HUVEC). HUVEC were divided into four groups: control; oxidative stress induction with 180 μM H2O2; treatment with 300 μM rutin; and concomitant induction with rutin and H2O2 for 24 hours. HUVEC treated with rutin produced higher amount of NO compared to control (P<0.01). In the oxidative stress-induced HUVEC, rutin successfully induced cells’ NO production (P<0.01). Rutin promoted NO production in HUVEC by inducing eNOS gene expression (P<0.05), eNOS protein synthesis (P<0.01), and eNOS activity (P<0.05). Treatment with rutin also led to increased gene and protein expression of basic fibroblast growth factor (bFGF) in HUVEC. Therefore, upregulation of eNOS expression by rutin may be mediated by bFGF. The results showed that rutin may improve endothelial function by augmenting NO production in human endothelial cells. |
| format | Article |
| id | doaj-art-10d97a8c98a749c28150a0f239473300 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-10d97a8c98a749c28150a0f2394733002025-02-03T05:43:57ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/169370169370Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial CellsAzizah Ugusman0Zaiton Zakaria1Kien Hui Chua2Nor Anita Megat Mohd Nordin3Zaleha Abdullah Mahdy4Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Obstetrics and Gynaecology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Cheras, Kuala Lumpur, MalaysiaNitric oxide (NO), produced by endothelial nitric oxide synthase (eNOS), is a major antiatherogenic factor in the blood vessel. Oxidative stress plays an important role in the pathogenesis of various cardiovascular diseases, including atherosclerosis. Decreased availability of endothelial NO promotes the progression of endothelial dysfunction and atherosclerosis. Rutin is a flavonoid with multiple cardiovascular protective effects. This study aimed to investigate the effects of rutin on eNOS and NO production in cultured human umbilical vein endothelial cells (HUVEC). HUVEC were divided into four groups: control; oxidative stress induction with 180 μM H2O2; treatment with 300 μM rutin; and concomitant induction with rutin and H2O2 for 24 hours. HUVEC treated with rutin produced higher amount of NO compared to control (P<0.01). In the oxidative stress-induced HUVEC, rutin successfully induced cells’ NO production (P<0.01). Rutin promoted NO production in HUVEC by inducing eNOS gene expression (P<0.05), eNOS protein synthesis (P<0.01), and eNOS activity (P<0.05). Treatment with rutin also led to increased gene and protein expression of basic fibroblast growth factor (bFGF) in HUVEC. Therefore, upregulation of eNOS expression by rutin may be mediated by bFGF. The results showed that rutin may improve endothelial function by augmenting NO production in human endothelial cells.http://dx.doi.org/10.1155/2014/169370 |
| spellingShingle | Azizah Ugusman Zaiton Zakaria Kien Hui Chua Nor Anita Megat Mohd Nordin Zaleha Abdullah Mahdy Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells The Scientific World Journal |
| title | Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells |
| title_full | Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells |
| title_fullStr | Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells |
| title_full_unstemmed | Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells |
| title_short | Role of Rutin on Nitric Oxide Synthesis in Human Umbilical Vein Endothelial Cells |
| title_sort | role of rutin on nitric oxide synthesis in human umbilical vein endothelial cells |
| url | http://dx.doi.org/10.1155/2014/169370 |
| work_keys_str_mv | AT azizahugusman roleofrutinonnitricoxidesynthesisinhumanumbilicalveinendothelialcells AT zaitonzakaria roleofrutinonnitricoxidesynthesisinhumanumbilicalveinendothelialcells AT kienhuichua roleofrutinonnitricoxidesynthesisinhumanumbilicalveinendothelialcells AT noranitamegatmohdnordin roleofrutinonnitricoxidesynthesisinhumanumbilicalveinendothelialcells AT zalehaabdullahmahdy roleofrutinonnitricoxidesynthesisinhumanumbilicalveinendothelialcells |