Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicks
This experiment was conducted to investigate the effects of adding Lactiplantibacillus plantarum to a low-protein diet on the growth performance, ability immune status, and intestinal microbiota of 0–21-day-old layer chickens. A total of 180 one-day-old healthy Hy-line brown laying chicks were rando...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1507752/full |
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| author | Lele Hou Huiling Qiu Jihong Dong Huawei Liu Shansong Gao Fu Chen |
| author_facet | Lele Hou Huiling Qiu Jihong Dong Huawei Liu Shansong Gao Fu Chen |
| author_sort | Lele Hou |
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| description | This experiment was conducted to investigate the effects of adding Lactiplantibacillus plantarum to a low-protein diet on the growth performance, ability immune status, and intestinal microbiota of 0–21-day-old layer chickens. A total of 180 one-day-old healthy Hy-line brown laying chicks were randomly divided into three groups with three replicates each of 20 chicks. The control group was fed a basal diet containing 19% protein, the low-protein (LP) group was fed a diet containing 17% protein, and the probiotic (LPL) group was fed with the 17% protein diet supplemented with L. plantarum (1.0 × 109 CFU/kg). The growth performance, antioxidant capacity, immune status, and gut microbiota of laying chickens were detected. We found that L. plantarum supplementation increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and levels of immunoglobulin (Ig) A, IgG, and interleukin-10 (IL-10) in serum of 17% protein +1.0 × 109 CFU/kg L. plantarum (LPL) compared to the 19% protein group (control). Furthermore, L. plantarum supplementation increased the liver index, GSH-Px and T-AOC activity in serum, and changed the microflora structure, diversity, and polyketose unit bioanabolic metabolism of 17% protein +1.0 × 109 CFU/kg L. plantarum (LPL) compared to the 17% protein group (LP). In conclusion, L. plantarum supplementation could compensate for the adverse effects of low-protein diets in chicks, and the combination of a low-protein diet and L. plantarum is a feasible way to reduce protein in the diet. |
| format | Article |
| id | doaj-art-10d517caaabc43039a9ad066d818d3d4 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-10d517caaabc43039a9ad066d818d3d42025-02-05T07:31:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15077521507752Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicksLele Hou0Huiling Qiu1Jihong Dong2Huawei Liu3Shansong Gao4Fu Chen5Institute of Animal Nutritional Metabolic Disease and Poisoning Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaInstitute of Animal Nutritional Metabolic Disease and Poisoning Disease, Haidu College, Qingdao Agricultural University, Laiyang, ChinaInstitute of Animal Nutritional Metabolic Disease and Poisoning Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaInstitute of Animal Nutritional Metabolic Disease and Poisoning Disease, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, ChinaInstitute of Animal Nutritional Metabolic Disease and Poisoning Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaInstitute of Animal Nutritional Metabolic Disease and Poisoning Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaThis experiment was conducted to investigate the effects of adding Lactiplantibacillus plantarum to a low-protein diet on the growth performance, ability immune status, and intestinal microbiota of 0–21-day-old layer chickens. A total of 180 one-day-old healthy Hy-line brown laying chicks were randomly divided into three groups with three replicates each of 20 chicks. The control group was fed a basal diet containing 19% protein, the low-protein (LP) group was fed a diet containing 17% protein, and the probiotic (LPL) group was fed with the 17% protein diet supplemented with L. plantarum (1.0 × 109 CFU/kg). The growth performance, antioxidant capacity, immune status, and gut microbiota of laying chickens were detected. We found that L. plantarum supplementation increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and levels of immunoglobulin (Ig) A, IgG, and interleukin-10 (IL-10) in serum of 17% protein +1.0 × 109 CFU/kg L. plantarum (LPL) compared to the 19% protein group (control). Furthermore, L. plantarum supplementation increased the liver index, GSH-Px and T-AOC activity in serum, and changed the microflora structure, diversity, and polyketose unit bioanabolic metabolism of 17% protein +1.0 × 109 CFU/kg L. plantarum (LPL) compared to the 17% protein group (LP). In conclusion, L. plantarum supplementation could compensate for the adverse effects of low-protein diets in chicks, and the combination of a low-protein diet and L. plantarum is a feasible way to reduce protein in the diet.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1507752/fullLactiplantibacillus plantarumlow-protein dietantioxidant capacityimmune statusgut microflora |
| spellingShingle | Lele Hou Huiling Qiu Jihong Dong Huawei Liu Shansong Gao Fu Chen Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicks Frontiers in Microbiology Lactiplantibacillus plantarum low-protein diet antioxidant capacity immune status gut microflora |
| title | Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicks |
| title_full | Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicks |
| title_fullStr | Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicks |
| title_full_unstemmed | Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicks |
| title_short | Lactiplantibacillus plantarum ameliorated the negative effects of a low-protein diet on growth performance, antioxidant capacity, immune status, and gut microbiota of laying chicks |
| title_sort | lactiplantibacillus plantarum ameliorated the negative effects of a low protein diet on growth performance antioxidant capacity immune status and gut microbiota of laying chicks |
| topic | Lactiplantibacillus plantarum low-protein diet antioxidant capacity immune status gut microflora |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1507752/full |
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