Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay
<b>Background/Objectives:</b> Metabolic profiling of human milk (HM) is indispensable for elucidating mother-milk-infant relationships. <b>Methods:</b> We evaluated the Biocrates MxP<sup>®</sup> Quant 500 assay for HM-targeted metabolomics (106 small molecules, 52...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/15/1/14 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587971839131648 |
|---|---|
| author | Daniela Hampel Setareh Shahab-Ferdows Gilberto Kac Lindsay H. Allen |
| author_facet | Daniela Hampel Setareh Shahab-Ferdows Gilberto Kac Lindsay H. Allen |
| author_sort | Daniela Hampel |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Metabolic profiling of human milk (HM) is indispensable for elucidating mother-milk-infant relationships. <b>Methods:</b> We evaluated the Biocrates MxP<sup>®</sup> Quant 500 assay for HM-targeted metabolomics (106 small molecules, 524 lipids) and analyzed in a feasibility test HM from apparently healthy Brazilian mothers (A: 2–8, B: 28–50, C: 88–119 days postpartum, n<sub>total</sub> = 25). <b>Results:</b> Of the 630 possible signatures detectable with this assay, 506 were above the limits of detection in an HM-pool (10 µL) used for assay evaluation, 12 of them above the upper limit of quantitation. Analyzing five different HM-pool volumes (2–20 µL) revealed acceptable linearity for 458 metabolites. Intraday accuracy of 80–120% was attained by 469 metabolites after spiking and for 342 after a 1:2 dilution. Analyzing HM from Brazilian mothers revealed significantly lower concentrations in colostrum vs. mature milk for many flow-injection analyses (FIA) and only a few LC-MS metabolites, including triglycerides, sphingomyelins, and phosphatidylcholines. Higher concentrations at the later lactation stages were found predominantly for amino acids and related compounds. <b>Conclusions:</b> The MxP Quant<sup>®</sup> 500 assay is a useful tool for HM metabolic profiling, minimizing analytical bias between matrices, and enhancing our ability to study milk as a biological system. |
| format | Article |
| id | doaj-art-106c98575f194ea781bcc12dff281abf |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-106c98575f194ea781bcc12dff281abf2025-01-24T13:41:10ZengMDPI AGMetabolites2218-19892025-01-011511410.3390/metabo15010014Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 AssayDaniela Hampel0Setareh Shahab-Ferdows1Gilberto Kac2Lindsay H. Allen3Department of Nutrition, University of California, Davis, CA 95616, USAUnited States Department of Agriculture, Agricultural Research Service—Western Human Nutrition Research Center, Davis, CA 95616, USANutrition Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilDepartment of Nutrition, University of California, Davis, CA 95616, USA<b>Background/Objectives:</b> Metabolic profiling of human milk (HM) is indispensable for elucidating mother-milk-infant relationships. <b>Methods:</b> We evaluated the Biocrates MxP<sup>®</sup> Quant 500 assay for HM-targeted metabolomics (106 small molecules, 524 lipids) and analyzed in a feasibility test HM from apparently healthy Brazilian mothers (A: 2–8, B: 28–50, C: 88–119 days postpartum, n<sub>total</sub> = 25). <b>Results:</b> Of the 630 possible signatures detectable with this assay, 506 were above the limits of detection in an HM-pool (10 µL) used for assay evaluation, 12 of them above the upper limit of quantitation. Analyzing five different HM-pool volumes (2–20 µL) revealed acceptable linearity for 458 metabolites. Intraday accuracy of 80–120% was attained by 469 metabolites after spiking and for 342 after a 1:2 dilution. Analyzing HM from Brazilian mothers revealed significantly lower concentrations in colostrum vs. mature milk for many flow-injection analyses (FIA) and only a few LC-MS metabolites, including triglycerides, sphingomyelins, and phosphatidylcholines. Higher concentrations at the later lactation stages were found predominantly for amino acids and related compounds. <b>Conclusions:</b> The MxP Quant<sup>®</sup> 500 assay is a useful tool for HM metabolic profiling, minimizing analytical bias between matrices, and enhancing our ability to study milk as a biological system.https://www.mdpi.com/2218-1989/15/1/14human milkcolostrumtargeted metaboliteslipidssmall moleculesLC-MS |
| spellingShingle | Daniela Hampel Setareh Shahab-Ferdows Gilberto Kac Lindsay H. Allen Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay Metabolites human milk colostrum targeted metabolites lipids small molecules LC-MS |
| title | Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay |
| title_full | Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay |
| title_fullStr | Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay |
| title_full_unstemmed | Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay |
| title_short | Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay |
| title_sort | evaluating metabolic profiling of human milk using biocrates mxp sup r sup quant 500 assay |
| topic | human milk colostrum targeted metabolites lipids small molecules LC-MS |
| url | https://www.mdpi.com/2218-1989/15/1/14 |
| work_keys_str_mv | AT danielahampel evaluatingmetabolicprofilingofhumanmilkusingbiocratesmxpsupsupquant500assay AT setarehshahabferdows evaluatingmetabolicprofilingofhumanmilkusingbiocratesmxpsupsupquant500assay AT gilbertokac evaluatingmetabolicprofilingofhumanmilkusingbiocratesmxpsupsupquant500assay AT lindsayhallen evaluatingmetabolicprofilingofhumanmilkusingbiocratesmxpsupsupquant500assay |