Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay

Evaluating Metabolic Profiling of Human Milk Using Biocrates MxP<sup>®</sup> QUANT 500 Assay

<b>Background/Objectives:</b> Metabolic profiling of human milk (HM) is indispensable for elucidating mother-milk-infant relationships. <b>Methods:</b> We evaluated the Biocrates MxP<sup>®</sup> Quant 500 assay for HM-targeted metabolomics (106 small molecules, 52...

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Bibliographic Details
Main Authors: Daniela Hampel, Setareh Shahab-Ferdows, Gilberto Kac, Lindsay H. Allen
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Metabolites
Subjects:
human milk
colostrum
targeted metabolites
lipids
small molecules
LC-MS
Online Access:https://www.mdpi.com/2218-1989/15/1/14
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Summary:<b>Background/Objectives:</b> Metabolic profiling of human milk (HM) is indispensable for elucidating mother-milk-infant relationships. <b>Methods:</b> We evaluated the Biocrates MxP<sup>®</sup> Quant 500 assay for HM-targeted metabolomics (106 small molecules, 524 lipids) and analyzed in a feasibility test HM from apparently healthy Brazilian mothers (A: 2–8, B: 28–50, C: 88–119 days postpartum, n<sub>total</sub> = 25). <b>Results:</b> Of the 630 possible signatures detectable with this assay, 506 were above the limits of detection in an HM-pool (10 µL) used for assay evaluation, 12 of them above the upper limit of quantitation. Analyzing five different HM-pool volumes (2–20 µL) revealed acceptable linearity for 458 metabolites. Intraday accuracy of 80–120% was attained by 469 metabolites after spiking and for 342 after a 1:2 dilution. Analyzing HM from Brazilian mothers revealed significantly lower concentrations in colostrum vs. mature milk for many flow-injection analyses (FIA) and only a few LC-MS metabolites, including triglycerides, sphingomyelins, and phosphatidylcholines. Higher concentrations at the later lactation stages were found predominantly for amino acids and related compounds. <b>Conclusions:</b> The MxP Quant<sup>®</sup> 500 assay is a useful tool for HM metabolic profiling, minimizing analytical bias between matrices, and enhancing our ability to study milk as a biological system.
ISSN:2218-1989

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