Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal
Dax1(also known as Nr0b1) is regarded as an important component of the transcription factor network in mouse embryonic stem cells (ESCs). However, the role and the molecular mechanism of Dax1 in the maintenance of different pluripotency states are poorly understood. Here, we constructed a stable Dax...
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/5522723 |
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| author | Jianrong He Yuda Cheng Yan Ruan Jiali Wang Yanping Tian Jiaqi Wang Fengsheng Wang Chen Zhang Yixiao Xu Lianlian Liu Meng Yu Jiangjun Wang Binyu Zhao Yue Zhang Yi Yang Gaoke Liu Wei Wu Ping He Jiaxiang Xiong He Huang Junlei Zhang Rui Jian |
| author_facet | Jianrong He Yuda Cheng Yan Ruan Jiali Wang Yanping Tian Jiaqi Wang Fengsheng Wang Chen Zhang Yixiao Xu Lianlian Liu Meng Yu Jiangjun Wang Binyu Zhao Yue Zhang Yi Yang Gaoke Liu Wei Wu Ping He Jiaxiang Xiong He Huang Junlei Zhang Rui Jian |
| author_sort | Jianrong He |
| collection | DOAJ |
| description | Dax1(also known as Nr0b1) is regarded as an important component of the transcription factor network in mouse embryonic stem cells (ESCs). However, the role and the molecular mechanism of Dax1 in the maintenance of different pluripotency states are poorly understood. Here, we constructed a stable Dax1 knockout (KO) cell line using the CRISPR/Cas9 system to analyze the precise function of Dax1. We reported that 2i/LIF-ESCs had significantly lower Dax1 expression than LIF/serum-ESCs. Dax1KO ES cell lines could be established in 2i/LIF and their pluripotency was confirmed. In contrast, Dax1-null ESCs could not be continuously passaged in LIF/serum due to severe differentiation and apoptosis. In LIF/serum, the activities of the Core module and Myc module were significantly reduced, while the PRC2 module was activated after Dax1KO. The expression of most proapoptotic genes and lineage-commitment genes were drastically increased, while the downregulated expression of antiapoptotic genes and many pluripotency genes was observed. Our research on the pluripotent state-dependent role of Dax1 provides clues to understand the molecular regulation mechanism at different stages of early embryonic development. |
| format | Article |
| id | doaj-art-10377facd0ba46299acaa6213344b66d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-10377facd0ba46299acaa6213344b66d2025-02-03T01:04:18ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/55227235522723Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-RenewalJianrong He0Yuda Cheng1Yan Ruan2Jiali Wang3Yanping Tian4Jiaqi Wang5Fengsheng Wang6Chen Zhang7Yixiao Xu8Lianlian Liu9Meng Yu10Jiangjun Wang11Binyu Zhao12Yue Zhang13Yi Yang14Gaoke Liu15Wei Wu16Ping He17Jiaxiang Xiong18He Huang19Junlei Zhang20Rui Jian21Department of Anesthesiology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaExperimental Center of Basic Medicine, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaThoracic Surgery Department, Southwest Hospital, The First Hospital Affiliated to Army Medical University, Chongqing 400038, ChinaCardiac Surgery Department, Southwest Hospital, The First Hospital Affiliated to Army Medical University, Chongqing 400038, ChinaExperimental Center of Basic Medicine, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, ChinaDepartment of Anesthesiology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaLaboratory of Stem Cell & Developmental Biology, Department of Histology and Embryology, Army Medical University, Chongqing 400038, ChinaDax1(also known as Nr0b1) is regarded as an important component of the transcription factor network in mouse embryonic stem cells (ESCs). However, the role and the molecular mechanism of Dax1 in the maintenance of different pluripotency states are poorly understood. Here, we constructed a stable Dax1 knockout (KO) cell line using the CRISPR/Cas9 system to analyze the precise function of Dax1. We reported that 2i/LIF-ESCs had significantly lower Dax1 expression than LIF/serum-ESCs. Dax1KO ES cell lines could be established in 2i/LIF and their pluripotency was confirmed. In contrast, Dax1-null ESCs could not be continuously passaged in LIF/serum due to severe differentiation and apoptosis. In LIF/serum, the activities of the Core module and Myc module were significantly reduced, while the PRC2 module was activated after Dax1KO. The expression of most proapoptotic genes and lineage-commitment genes were drastically increased, while the downregulated expression of antiapoptotic genes and many pluripotency genes was observed. Our research on the pluripotent state-dependent role of Dax1 provides clues to understand the molecular regulation mechanism at different stages of early embryonic development.http://dx.doi.org/10.1155/2021/5522723 |
| spellingShingle | Jianrong He Yuda Cheng Yan Ruan Jiali Wang Yanping Tian Jiaqi Wang Fengsheng Wang Chen Zhang Yixiao Xu Lianlian Liu Meng Yu Jiangjun Wang Binyu Zhao Yue Zhang Yi Yang Gaoke Liu Wei Wu Ping He Jiaxiang Xiong He Huang Junlei Zhang Rui Jian Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal Stem Cells International |
| title | Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal |
| title_full | Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal |
| title_fullStr | Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal |
| title_full_unstemmed | Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal |
| title_short | Pluripotency-State-Dependent Role of Dax1 in Embryonic Stem Cells Self-Renewal |
| title_sort | pluripotency state dependent role of dax1 in embryonic stem cells self renewal |
| url | http://dx.doi.org/10.1155/2021/5522723 |
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