Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection
In this study, mouse MLN cells and thymocytes from advanced stages of LP-BM5 retrovirus infection were studied. A decrease in the percentage of IL-7+ cells and an increase in the percentage of IL-16+ cells in the MLN indicated that sec...
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Wiley
2005-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520500303339 |
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| author | Maria C. Lopez Ronald R. Watson |
| author_facet | Maria C. Lopez Ronald R. Watson |
| author_sort | Maria C. Lopez |
| collection | DOAJ |
| description | In this study, mouse MLN cells and thymocytes from advanced stages of
LP-BM5 retrovirus infection were studied. A decrease in
the percentage of IL-7+
cells and an increase in the percentage of IL-16+ cells in the MLN
indicated that
secretion of these cytokines was also altered after LP-BM5 infection. The
percentage of MLN T cells expressing IL-7 receptors was significantly reduced,
while the percentage of MLN T cells expressing TNFR-p75 and of B cells
expressing TNFR-p55 increased. Simultaneous analysis of surface markers and
cytokine secretion was done in an attempt to understand whether the deregulation
of IFN-Υ secretion could be ascribed to a defined cell phenotype, concluding
that
all T cell subsets studied increased IFN-Υ secretion after retrovirus infection.
Finally,
thymocyte phenotype was further analyzed trying to correlate changes in thymocyte
phenotype with MLN cell phenotype. The results indicated that the increase in
single positive either CD4+CD8- or CD4-
CD8+ cells was due to accumulation of
both immature (CD3- ) and mature (CD3+) single
positive thymocytes. Moreover,
single positive mature thymocytes presented a phenotype similar to the phenotype
previously seen on MLN T cells. In summary, we can conclude that LP-BM5 uses
the immune system to reach the thymus where it interferes with the generation of
functionally mature T cells, favoring the development of T cells with an abnormal
phenotype. These new T cells are activated to secrete several cytokines that in turn
will favor retrovirus
replication and inhibit any attempt of the immune system to control infection. |
| format | Article |
| id | doaj-art-1024d68977ce4dc78e8b683aa84df415 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-1024d68977ce4dc78e8b683aa84df4152025-02-03T01:11:31ZengWileyClinical and Developmental Immunology1740-25221740-25302005-01-0112424925710.1080/17402520500303339Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus InfectionMaria C. Lopez0Ronald R. Watson1Health Promotion Sciences, Enid and Mel Zuckerman College of Public Health, The University of Arizona, Tucson, AZ 85724, USAHealth Promotion Sciences, Enid and Mel Zuckerman College of Public Health, The University of Arizona, Tucson, AZ 85724, USAIn this study, mouse MLN cells and thymocytes from advanced stages of LP-BM5 retrovirus infection were studied. A decrease in the percentage of IL-7+ cells and an increase in the percentage of IL-16+ cells in the MLN indicated that secretion of these cytokines was also altered after LP-BM5 infection. The percentage of MLN T cells expressing IL-7 receptors was significantly reduced, while the percentage of MLN T cells expressing TNFR-p75 and of B cells expressing TNFR-p55 increased. Simultaneous analysis of surface markers and cytokine secretion was done in an attempt to understand whether the deregulation of IFN-Υ secretion could be ascribed to a defined cell phenotype, concluding that all T cell subsets studied increased IFN-Υ secretion after retrovirus infection. Finally, thymocyte phenotype was further analyzed trying to correlate changes in thymocyte phenotype with MLN cell phenotype. The results indicated that the increase in single positive either CD4+CD8- or CD4- CD8+ cells was due to accumulation of both immature (CD3- ) and mature (CD3+) single positive thymocytes. Moreover, single positive mature thymocytes presented a phenotype similar to the phenotype previously seen on MLN T cells. In summary, we can conclude that LP-BM5 uses the immune system to reach the thymus where it interferes with the generation of functionally mature T cells, favoring the development of T cells with an abnormal phenotype. These new T cells are activated to secrete several cytokines that in turn will favor retrovirus replication and inhibit any attempt of the immune system to control infection.http://dx.doi.org/10.1080/17402520500303339 |
| spellingShingle | Maria C. Lopez Ronald R. Watson Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection Clinical and Developmental Immunology |
| title | Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection |
| title_full | Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection |
| title_fullStr | Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection |
| title_full_unstemmed | Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection |
| title_short | Alterations in Mesenteric Lymph Node T Cell Phenotype and Cytokine Secretion are Associated with Changes in Thymocyte Phenotype after LP-BM5 Retrovirus Infection |
| title_sort | alterations in mesenteric lymph node t cell phenotype and cytokine secretion are associated with changes in thymocyte phenotype after lp bm5 retrovirus infection |
| url | http://dx.doi.org/10.1080/17402520500303339 |
| work_keys_str_mv | AT mariaclopez alterationsinmesentericlymphnodetcellphenotypeandcytokinesecretionareassociatedwithchangesinthymocytephenotypeafterlpbm5retrovirusinfection AT ronaldrwatson alterationsinmesentericlymphnodetcellphenotypeandcytokinesecretionareassociatedwithchangesinthymocytephenotypeafterlpbm5retrovirusinfection |