Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus
Plumb Beach, Brooklyn, New York in USA is an important horseshoe crab breeding and nursery ground that has experienced substantial anthropogenic influence, including pollution, erosion and subsequent restoration. Since little is known about the relationship between sediment microbial communities and...
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2020-07-01
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| Series: | Global Journal of Environmental Science and Management |
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| Online Access: | https://www.gjesm.net/article_38725_0dc990304bd9c9582ba5cf59d273eb35.pdf |
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| author | J. Petersen C.P. Colon J.L. Joyner |
| author_facet | J. Petersen C.P. Colon J.L. Joyner |
| author_sort | J. Petersen |
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| description | Plumb Beach, Brooklyn, New York in USA is an important horseshoe crab breeding and nursery ground that has experienced substantial anthropogenic influence, including pollution, erosion and subsequent restoration. Since little is known about the relationship between sediment microbial communities and juvenile horseshoe crab survival, next generation sequencing was used to characterize and compare the sediment microbiome of three distinct areas of Plumb Beach:- a tidal creek with abundant juveniles, East Beach with moderate number of juveniles, and West Beach- a highly disturbed area where juvenile crabs are rarely seen. The microbiome of juvenile crab intestinal content (both dissected gut content and fecal flush content) from the tidal creek site was also examined. The results showed that in our 2017 survey, the overall dominant sediment orders at all beach sites were Vibrionales (30%), Flavobacteriales (22%) and Alteromonadales (21%). Although alpha diversity was similar among the three beach sites, Bray-Curtis distances assessed by Permanova revealed significant differences in Beta diversity, with a unique microbial assemblage found in the tidal creek. Both crab gut and fecal flush samples did not sequence well, showing low species diversity and very high variability. This study is the first to use next generation sequencing to characterize Plumb Beach sediment microbes and the first attempt to examine the gut microbiome of juvenile horseshoe crabs. This information will contribute to understanding the relationships between sediment microbial assemblages and juvenile crab populations within this important urban habitat. |
| format | Article |
| id | doaj-art-101fae635e504246aefb5b4eb7bd7eb9 |
| institution | Kabale University |
| issn | 2383-3572 2383-3866 |
| language | English |
| publishDate | 2020-07-01 |
| publisher | GJESM Publisher |
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| series | Global Journal of Environmental Science and Management |
| spelling | doaj-art-101fae635e504246aefb5b4eb7bd7eb92025-02-02T01:22:09ZengGJESM PublisherGlobal Journal of Environmental Science and Management2383-35722383-38662020-07-016330932210.22034/gjesm.2020.03.0338725Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemusJ. Petersen0C.P. Colon1J.L. Joyner2Department of Biological Sciences, Queensborough Community College, City University of New York, Bayside, New York, USADepartment of Biological Sciences, Kingsborough Community College, City University of New York, Brooklyn, New York, USADepartment of Biology, Georgia State University, Atlanta, Georgia, USAGeorgia State UniversityPlumb Beach, Brooklyn, New York in USA is an important horseshoe crab breeding and nursery ground that has experienced substantial anthropogenic influence, including pollution, erosion and subsequent restoration. Since little is known about the relationship between sediment microbial communities and juvenile horseshoe crab survival, next generation sequencing was used to characterize and compare the sediment microbiome of three distinct areas of Plumb Beach:- a tidal creek with abundant juveniles, East Beach with moderate number of juveniles, and West Beach- a highly disturbed area where juvenile crabs are rarely seen. The microbiome of juvenile crab intestinal content (both dissected gut content and fecal flush content) from the tidal creek site was also examined. The results showed that in our 2017 survey, the overall dominant sediment orders at all beach sites were Vibrionales (30%), Flavobacteriales (22%) and Alteromonadales (21%). Although alpha diversity was similar among the three beach sites, Bray-Curtis distances assessed by Permanova revealed significant differences in Beta diversity, with a unique microbial assemblage found in the tidal creek. Both crab gut and fecal flush samples did not sequence well, showing low species diversity and very high variability. This study is the first to use next generation sequencing to characterize Plumb Beach sediment microbes and the first attempt to examine the gut microbiome of juvenile horseshoe crabs. This information will contribute to understanding the relationships between sediment microbial assemblages and juvenile crab populations within this important urban habitat.https://www.gjesm.net/article_38725_0dc990304bd9c9582ba5cf59d273eb35.pdfbeach restorationbeach sedimenthorseshoe crab microbiomeintertidal zonejuvenile horseshoe crab habitatlittoral zone |
| spellingShingle | J. Petersen C.P. Colon J.L. Joyner Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus Global Journal of Environmental Science and Management beach restoration beach sediment horseshoe crab microbiome intertidal zone juvenile horseshoe crab habitat littoral zone |
| title | Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus |
| title_full | Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus |
| title_fullStr | Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus |
| title_full_unstemmed | Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus |
| title_short | Sediment microbiomes associated with critical habitat of the Juvenile American Horseshoe Crab; Limulus polyphemus |
| title_sort | sediment microbiomes associated with critical habitat of the juvenile american horseshoe crab limulus polyphemus |
| topic | beach restoration beach sediment horseshoe crab microbiome intertidal zone juvenile horseshoe crab habitat littoral zone |
| url | https://www.gjesm.net/article_38725_0dc990304bd9c9582ba5cf59d273eb35.pdf |
| work_keys_str_mv | AT jpetersen sedimentmicrobiomesassociatedwithcriticalhabitatofthejuvenileamericanhorseshoecrablimuluspolyphemus AT cpcolon sedimentmicrobiomesassociatedwithcriticalhabitatofthejuvenileamericanhorseshoecrablimuluspolyphemus AT jljoyner sedimentmicrobiomesassociatedwithcriticalhabitatofthejuvenileamericanhorseshoecrablimuluspolyphemus |