Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner
The spiral ganglion neurons (SGNs) are the primary afferent neurons in the spiral ganglion (SG), while their degeneration or loss would cause sensorineural hearing loss. As a cardiac-derived hormone, atrial natriuretic peptide (ANP) plays a critical role in cardiovascular homeostasis through binding...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/8831735 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568616476737536 |
|---|---|
| author | Fei Sun Ke Zhou Ke-yong Tian Jie Wang Jian-hua Qiu Ding-jun Zha |
| author_facet | Fei Sun Ke Zhou Ke-yong Tian Jie Wang Jian-hua Qiu Ding-jun Zha |
| author_sort | Fei Sun |
| collection | DOAJ |
| description | The spiral ganglion neurons (SGNs) are the primary afferent neurons in the spiral ganglion (SG), while their degeneration or loss would cause sensorineural hearing loss. As a cardiac-derived hormone, atrial natriuretic peptide (ANP) plays a critical role in cardiovascular homeostasis through binding to its functional receptors (NPR-A and NPR-C). ANP and its receptors are widely expressed in the mammalian nervous system where they could be implicated in the regulation of multiple neural functions. Although previous studies have provided direct evidence for the presence of ANP and its functional receptors in the inner ear, their presence within the cochlear SG and their regulatory roles during auditory neurotransmission and development remain largely unknown. Based on our previous findings, we investigated the expression patterns of ANP and its receptors in the cochlear SG and dissociated SGNs and determined the influence of ANP on neurite outgrowth in vitro by using organotypic SG explants and dissociated SGN cultures from postnatal rats. We have demonstrated that ANP and its receptors are expressed in neurons within the cochlear SG of postnatal rat, while ANP may promote neurite outgrowth of SGNs via the NPR-A/cGMP/PKG pathway in a dose-dependent manner. These results indicate that ANP would play a role in normal neuritogenesis of SGN during cochlear development and represents a potential therapeutic candidate to enhance regeneration and regrowth of SGN neurites. |
| format | Article |
| id | doaj-art-101f3891506e4a7ab4e5f8f7df69d304 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-101f3891506e4a7ab4e5f8f7df69d3042025-02-03T00:58:42ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/88317358831735Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent MannerFei Sun0Ke Zhou1Ke-yong Tian2Jie Wang3Jian-hua Qiu4Ding-jun Zha5Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaCenter of Clinical Laboratory Medicine of PLA, Department of Laboratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Otolaryngology-Head and Neck Surgery, The Affiliated Children Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710003, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710032, ChinaThe spiral ganglion neurons (SGNs) are the primary afferent neurons in the spiral ganglion (SG), while their degeneration or loss would cause sensorineural hearing loss. As a cardiac-derived hormone, atrial natriuretic peptide (ANP) plays a critical role in cardiovascular homeostasis through binding to its functional receptors (NPR-A and NPR-C). ANP and its receptors are widely expressed in the mammalian nervous system where they could be implicated in the regulation of multiple neural functions. Although previous studies have provided direct evidence for the presence of ANP and its functional receptors in the inner ear, their presence within the cochlear SG and their regulatory roles during auditory neurotransmission and development remain largely unknown. Based on our previous findings, we investigated the expression patterns of ANP and its receptors in the cochlear SG and dissociated SGNs and determined the influence of ANP on neurite outgrowth in vitro by using organotypic SG explants and dissociated SGN cultures from postnatal rats. We have demonstrated that ANP and its receptors are expressed in neurons within the cochlear SG of postnatal rat, while ANP may promote neurite outgrowth of SGNs via the NPR-A/cGMP/PKG pathway in a dose-dependent manner. These results indicate that ANP would play a role in normal neuritogenesis of SGN during cochlear development and represents a potential therapeutic candidate to enhance regeneration and regrowth of SGN neurites.http://dx.doi.org/10.1155/2020/8831735 |
| spellingShingle | Fei Sun Ke Zhou Ke-yong Tian Jie Wang Jian-hua Qiu Ding-jun Zha Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner Neural Plasticity |
| title | Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner |
| title_full | Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner |
| title_fullStr | Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner |
| title_full_unstemmed | Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner |
| title_short | Atrial Natriuretic Peptide Improves Neurite Outgrowth from Spiral Ganglion Neurons In Vitro through a cGMP-Dependent Manner |
| title_sort | atrial natriuretic peptide improves neurite outgrowth from spiral ganglion neurons in vitro through a cgmp dependent manner |
| url | http://dx.doi.org/10.1155/2020/8831735 |
| work_keys_str_mv | AT feisun atrialnatriureticpeptideimprovesneuriteoutgrowthfromspiralganglionneuronsinvitrothroughacgmpdependentmanner AT kezhou atrialnatriureticpeptideimprovesneuriteoutgrowthfromspiralganglionneuronsinvitrothroughacgmpdependentmanner AT keyongtian atrialnatriureticpeptideimprovesneuriteoutgrowthfromspiralganglionneuronsinvitrothroughacgmpdependentmanner AT jiewang atrialnatriureticpeptideimprovesneuriteoutgrowthfromspiralganglionneuronsinvitrothroughacgmpdependentmanner AT jianhuaqiu atrialnatriureticpeptideimprovesneuriteoutgrowthfromspiralganglionneuronsinvitrothroughacgmpdependentmanner AT dingjunzha atrialnatriureticpeptideimprovesneuriteoutgrowthfromspiralganglionneuronsinvitrothroughacgmpdependentmanner |