Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging
Abstract Human cerebral organoids serve as a quintessential model for deciphering the complexities of brain development in a three-dimensional milieu. However, imaging these organoids, particularly when they exceed several millimeters in size, has been curtailed by the technical impediments such as...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-85455-8 |
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| author | Sangjun Park Cheol Hong Min Eunjin Choi Jeong-Sun Choi Kyungjin Park Seokyoung Han Wonjun Choi Hyun-Jong Jang Kyung-Ok Cho Moonseok Kim |
| author_facet | Sangjun Park Cheol Hong Min Eunjin Choi Jeong-Sun Choi Kyungjin Park Seokyoung Han Wonjun Choi Hyun-Jong Jang Kyung-Ok Cho Moonseok Kim |
| author_sort | Sangjun Park |
| collection | DOAJ |
| description | Abstract Human cerebral organoids serve as a quintessential model for deciphering the complexities of brain development in a three-dimensional milieu. However, imaging these organoids, particularly when they exceed several millimeters in size, has been curtailed by the technical impediments such as phototoxicity, slow imaging speeds, and inadequate resolution and imaging depth. Addressing these pivotal challenges, our study has pioneered a high-speed scanning microscope, synergistically coupled with advanced computational image processing. This ensemble has empowered us to monitor the intricate dynamics of neuron and oligodendrocyte development within cerebral organoids across a trajectory of approximately two months. Line-shaped illumination mitigates photodamage and, alongside refined spatial gating, maximizes signal collection through integrating with computational processing. The integration of deconvolution and compressive sensing has improved image contrast by 6-fold, elucidating fine features of the neurites. Thus, noninvasive imaging enabled us to perform long-term tracking of neural and oligodendroglial development in the large-scale human cerebral organoid. Furthermore, our sophisticated volumetric segmentation algorithm has yielded a robust four-dimensional quantitative analysis, encapsulating both neuronal and oligodendroglial maturation. Collectively, these advances mark a significant advancement in the field of neurodevelopment, providing a powerful tool for in-depth study of complex brain organoid systems. |
| format | Article |
| id | doaj-art-100d9f089b16489baa7d1615c8fae140 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-100d9f089b16489baa7d1615c8fae1402025-01-26T12:25:10ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-025-85455-8Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imagingSangjun Park0Cheol Hong Min1Eunjin Choi2Jeong-Sun Choi3Kyungjin Park4Seokyoung Han5Wonjun Choi6Hyun-Jong Jang7Kyung-Ok Cho8Moonseok Kim9Department of Medical Life Sciences, College of Medicine, The Catholic University of KoreaDepartment of Medical Life Sciences, College of Medicine, The Catholic University of KoreaDepartment of Medical Life Sciences, College of Medicine, The Catholic University of KoreaDepartment of Pharmacology, College of Medicine, The Catholic University of KoreaDepartment of Medical Life Sciences, College of Medicine, The Catholic University of KoreaDepartment of Medical Life Sciences, College of Medicine, The Catholic University of KoreaPark Systems CorpDepartment of Medical Sciences, College of Medicine, The Catholic University of KoreaDepartment of Medical Sciences, College of Medicine, The Catholic University of KoreaDepartment of Medical Life Sciences, College of Medicine, The Catholic University of KoreaAbstract Human cerebral organoids serve as a quintessential model for deciphering the complexities of brain development in a three-dimensional milieu. However, imaging these organoids, particularly when they exceed several millimeters in size, has been curtailed by the technical impediments such as phototoxicity, slow imaging speeds, and inadequate resolution and imaging depth. Addressing these pivotal challenges, our study has pioneered a high-speed scanning microscope, synergistically coupled with advanced computational image processing. This ensemble has empowered us to monitor the intricate dynamics of neuron and oligodendrocyte development within cerebral organoids across a trajectory of approximately two months. Line-shaped illumination mitigates photodamage and, alongside refined spatial gating, maximizes signal collection through integrating with computational processing. The integration of deconvolution and compressive sensing has improved image contrast by 6-fold, elucidating fine features of the neurites. Thus, noninvasive imaging enabled us to perform long-term tracking of neural and oligodendroglial development in the large-scale human cerebral organoid. Furthermore, our sophisticated volumetric segmentation algorithm has yielded a robust four-dimensional quantitative analysis, encapsulating both neuronal and oligodendroglial maturation. Collectively, these advances mark a significant advancement in the field of neurodevelopment, providing a powerful tool for in-depth study of complex brain organoid systems.https://doi.org/10.1038/s41598-025-85455-8Cerebral organoidsCortical developmentNoninvasiveVolumetric imagingImage processing |
| spellingShingle | Sangjun Park Cheol Hong Min Eunjin Choi Jeong-Sun Choi Kyungjin Park Seokyoung Han Wonjun Choi Hyun-Jong Jang Kyung-Ok Cho Moonseok Kim Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging Scientific Reports Cerebral organoids Cortical development Noninvasive Volumetric imaging Image processing |
| title | Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging |
| title_full | Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging |
| title_fullStr | Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging |
| title_full_unstemmed | Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging |
| title_short | Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging |
| title_sort | long term tracking of neural and oligodendroglial development in large scale human cerebral organoids by noninvasive volumetric imaging |
| topic | Cerebral organoids Cortical development Noninvasive Volumetric imaging Image processing |
| url | https://doi.org/10.1038/s41598-025-85455-8 |
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