Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential
In the current study, parietin was extracted aerobically from lichen and quantified using parietin activity tests. Different parameters were optimized to extract parietin, viz. suspending 1.5 g biomass of lichen in 15 mL methanol and incubating it for 45 min at 37 °C. Parietin-mediated AgNPs were sy...
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Elsevier
2025-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221171562400657X |
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| author | Sikander Ali Alisha Moazzam Saima Shahzad Mirza Majid Mahmood Sibtain Ahmed Muhammad Usman Ahmad Gildardo Rivera Akhtar Rasool Asif Muhammad Arshad Mirza Imran Shahzad Kaynat William Sundas Sharif |
| author_facet | Sikander Ali Alisha Moazzam Saima Shahzad Mirza Majid Mahmood Sibtain Ahmed Muhammad Usman Ahmad Gildardo Rivera Akhtar Rasool Asif Muhammad Arshad Mirza Imran Shahzad Kaynat William Sundas Sharif |
| author_sort | Sikander Ali |
| collection | DOAJ |
| description | In the current study, parietin was extracted aerobically from lichen and quantified using parietin activity tests. Different parameters were optimized to extract parietin, viz. suspending 1.5 g biomass of lichen in 15 mL methanol and incubating it for 45 min at 37 °C. Parietin-mediated AgNPs were synthesized by 30-minute procurement period with 3 mL of AgNO3 (3 mM) solution. Visual observations and ultraviolet–visible (UV–Vis) spectra demonstrated that the Ag precursor’s treatment changed the color of fresh methanolic extract to greenish-brown. Fourier transfer infrared (FTIR) spectroscopy was used to identify lichen secondary metabolites. The effective synthesis of Xa-AgNPs was further validated by X-ray diffraction patterns and scanning electron microscopy. The free parietin extract and synthesized nanoparticles (Xa-AgNPs) were evaluated for antimicrobial activity against selected bacterial isolates; S. aureus, B. subtilis (gram-positive), E. coli, Pseudomonas sp. (gram-negative) by agar well diffusion method. The results indicated that free parietin extract and synthesized nanoparticles (Xa-AgNPs) have potential antimicrobial activity against selected gram-positive and gram-negative bacterial isolates. The current results exhibited that Xa-AgNPs are potent bactericidal agents and should be considered a promising candidate to combat bacterial infections. |
| format | Article |
| id | doaj-art-1003c4ff19f349459cec3de91aa3d454 |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-1003c4ff19f349459cec3de91aa3d4542025-01-29T05:00:42ZengElsevierResults in Chemistry2211-71562025-01-0113101961Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potentialSikander Ali0Alisha Moazzam1Saima Shahzad Mirza2Majid Mahmood3Sibtain Ahmed4Muhammad Usman Ahmad5Gildardo Rivera6Akhtar Rasool Asif7Muhammad Arshad8Mirza Imran Shahzad9Kaynat William10Sundas Sharif11Department of Microbiology, Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University, Lahore 54000, Pakistan; Corresponding authors.Department of Microbiology, Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University, Lahore 54000, PakistanMicrobial Bioengineering Laboratory, Department of Zoology, University of Education, Bank Road Campus, Lahore, PakistanDepartment of Zoology, University of Poonch Rawalakot, Azad Jammu and Kashmir, PakistanDepartment of Biochemistry, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Microbiology, Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University, Lahore 54000, PakistanLaboratorio de Biotecnología Farmacéutica, Centro de Biotecnología Genómica, Instituto Politécnico Nacional, México; Corresponding authors.Animal Breeding and Genetics Section, Jhang-Campus, University of Veterinary and Animal Sciences, Lahore, Pakistan; Key Laboratory of Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Wuhan, ChinaDepartment of Basic Sciences, Jhang-campus, University of Veterinary and Animal Sciences, Lahore 54000, PakistanDepartment of Biochemistry and Molecular Biology, The Islamia University, Bahawalpur, PakistanDepartment of Microbiology, Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University, Lahore 54000, PakistanDepartment of Microbiology, Dr. Ikram-ul-Haq Institute of Industrial Biotechnology, Government College University, Lahore 54000, PakistanIn the current study, parietin was extracted aerobically from lichen and quantified using parietin activity tests. Different parameters were optimized to extract parietin, viz. suspending 1.5 g biomass of lichen in 15 mL methanol and incubating it for 45 min at 37 °C. Parietin-mediated AgNPs were synthesized by 30-minute procurement period with 3 mL of AgNO3 (3 mM) solution. Visual observations and ultraviolet–visible (UV–Vis) spectra demonstrated that the Ag precursor’s treatment changed the color of fresh methanolic extract to greenish-brown. Fourier transfer infrared (FTIR) spectroscopy was used to identify lichen secondary metabolites. The effective synthesis of Xa-AgNPs was further validated by X-ray diffraction patterns and scanning electron microscopy. The free parietin extract and synthesized nanoparticles (Xa-AgNPs) were evaluated for antimicrobial activity against selected bacterial isolates; S. aureus, B. subtilis (gram-positive), E. coli, Pseudomonas sp. (gram-negative) by agar well diffusion method. The results indicated that free parietin extract and synthesized nanoparticles (Xa-AgNPs) have potential antimicrobial activity against selected gram-positive and gram-negative bacterial isolates. The current results exhibited that Xa-AgNPs are potent bactericidal agents and should be considered a promising candidate to combat bacterial infections.http://www.sciencedirect.com/science/article/pii/S221171562400657XBio-fabricationLichenBiomassImmobilizationAnthraquinone |
| spellingShingle | Sikander Ali Alisha Moazzam Saima Shahzad Mirza Majid Mahmood Sibtain Ahmed Muhammad Usman Ahmad Gildardo Rivera Akhtar Rasool Asif Muhammad Arshad Mirza Imran Shahzad Kaynat William Sundas Sharif Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential Results in Chemistry Bio-fabrication Lichen Biomass Immobilization Anthraquinone |
| title | Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential |
| title_full | Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential |
| title_fullStr | Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential |
| title_full_unstemmed | Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential |
| title_short | Exploring green synthesis of parietin-mediated silver nanoparticles using γ-ray induced Xanthoria parietina foliose extracts for antibacterial potential |
| title_sort | exploring green synthesis of parietin mediated silver nanoparticles using γ ray induced xanthoria parietina foliose extracts for antibacterial potential |
| topic | Bio-fabrication Lichen Biomass Immobilization Anthraquinone |
| url | http://www.sciencedirect.com/science/article/pii/S221171562400657X |
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