Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming
Reprogramming to pluripotency involves drastic restructuring of both metabolism and the epigenome. However, induced pluripotent stem cells (iPSC) retain transcriptional memory, epigenetic memory, and metabolic memory from their somatic cells of origin and acquire aberrant characteristics distinct fr...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/7360121 |
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| author | James Spyrou David K. Gardner Alexandra J. Harvey |
| author_facet | James Spyrou David K. Gardner Alexandra J. Harvey |
| author_sort | James Spyrou |
| collection | DOAJ |
| description | Reprogramming to pluripotency involves drastic restructuring of both metabolism and the epigenome. However, induced pluripotent stem cells (iPSC) retain transcriptional memory, epigenetic memory, and metabolic memory from their somatic cells of origin and acquire aberrant characteristics distinct from either other pluripotent cells or parental cells, reflecting incomplete reprogramming. As a critical link between the microenvironment and regulation of the epigenome, nutrient availability likely plays a significant role in the retention of somatic cell memory by iPSC. Significantly, relative nutrient availability impacts iPSC reprogramming efficiency, epigenetic regulation and cell fate, and differentially alters their ability to respond to physiological stimuli. The significance of metabolites during the reprogramming process is central to further elucidating how iPSC retain somatic cell characteristics and optimising culture conditions to generate iPSC with physiological phenotypes to ensure their reliable use in basic research and clinical applications. This review serves to integrate studies on iPSC reprogramming, memory retention and metabolism, and identifies areas in which current knowledge is limited. |
| format | Article |
| id | doaj-art-0ffc2e0bc8424c67aab1e40e58847142 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0ffc2e0bc8424c67aab1e40e588471422025-02-03T01:23:44ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/73601217360121Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell ReprogrammingJames Spyrou0David K. Gardner1Alexandra J. Harvey2School of BioSciences, The University of Melbourne, Parkville, VIC 3010, AustraliaSchool of BioSciences, The University of Melbourne, Parkville, VIC 3010, AustraliaSchool of BioSciences, The University of Melbourne, Parkville, VIC 3010, AustraliaReprogramming to pluripotency involves drastic restructuring of both metabolism and the epigenome. However, induced pluripotent stem cells (iPSC) retain transcriptional memory, epigenetic memory, and metabolic memory from their somatic cells of origin and acquire aberrant characteristics distinct from either other pluripotent cells or parental cells, reflecting incomplete reprogramming. As a critical link between the microenvironment and regulation of the epigenome, nutrient availability likely plays a significant role in the retention of somatic cell memory by iPSC. Significantly, relative nutrient availability impacts iPSC reprogramming efficiency, epigenetic regulation and cell fate, and differentially alters their ability to respond to physiological stimuli. The significance of metabolites during the reprogramming process is central to further elucidating how iPSC retain somatic cell characteristics and optimising culture conditions to generate iPSC with physiological phenotypes to ensure their reliable use in basic research and clinical applications. This review serves to integrate studies on iPSC reprogramming, memory retention and metabolism, and identifies areas in which current knowledge is limited.http://dx.doi.org/10.1155/2019/7360121 |
| spellingShingle | James Spyrou David K. Gardner Alexandra J. Harvey Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming Stem Cells International |
| title | Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming |
| title_full | Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming |
| title_fullStr | Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming |
| title_full_unstemmed | Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming |
| title_short | Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming |
| title_sort | metabolism is a key regulator of induced pluripotent stem cell reprogramming |
| url | http://dx.doi.org/10.1155/2019/7360121 |
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