M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin
Tumor microenvironment components dictate the growth and progression of various cancers. Tumor-associated macrophages are the most predominant cells in TME and play a major role in cancer invasiveness. Gastric cancer is one of the most common cancers in Asia, and recently, various cases of resistanc...
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2020-01-01
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Series: | Journal of Immunology Research |
Online Access: | http://dx.doi.org/10.1155/2020/1731457 |
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author | Daniel Ngabire Irvine Niyonizigiye Maheshkumar Prakash Patil Yeong-Ae Seong Yong Bae Seo Gun-Do Kim |
author_facet | Daniel Ngabire Irvine Niyonizigiye Maheshkumar Prakash Patil Yeong-Ae Seong Yong Bae Seo Gun-Do Kim |
author_sort | Daniel Ngabire |
collection | DOAJ |
description | Tumor microenvironment components dictate the growth and progression of various cancers. Tumor-associated macrophages are the most predominant cells in TME and play a major role in cancer invasiveness. Gastric cancer is one of the most common cancers in Asia, and recently, various cases of resistance to fluorouracil treatment have been reported. In this study, we investigated the role of alternatively activated macrophages in the resistance of AGS gastric cancer cells to fluorouracil. THP-1 cells were polarized using recombinant human IL-4, then were cocultured with AGS cells treated with fluorouracil. Cell viability, Western blot, immunofluorescence, and cell invasion were performed for this investigation. Our results demonstrated that polarized macrophages initiated the survival of treated AGS cells and induced the resistance in AGS by upregulating the expression of integrin β3, focal adhesion protein (FAK), and cofilin proteins. These results reveal that integrin β3, focal adhesion protein (FAK), and cofilin proteins are potential targets for the improvement of fluorouracil efficacy in gastric cancer treatment. |
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id | doaj-art-0febb3d9ce0341a393882bf70b5b062f |
institution | Kabale University |
issn | 2314-8861 2314-7156 |
language | English |
publishDate | 2020-01-01 |
publisher | Wiley |
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series | Journal of Immunology Research |
spelling | doaj-art-0febb3d9ce0341a393882bf70b5b062f2025-02-03T06:05:38ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/17314571731457M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and CofilinDaniel Ngabire0Irvine Niyonizigiye1Maheshkumar Prakash Patil2Yeong-Ae Seong3Yong Bae Seo4Gun-Do Kim5Department of Microbiology, College of Natural Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of KoreaDepartment of Microbiology, College of Natural Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of KoreaResearch Institute for Basic Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of KoreaDepartment of Microbiology, College of Natural Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of KoreaInstitute for Marine Biotechnology, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of KoreaDepartment of Microbiology, College of Natural Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan 48513, Republic of KoreaTumor microenvironment components dictate the growth and progression of various cancers. Tumor-associated macrophages are the most predominant cells in TME and play a major role in cancer invasiveness. Gastric cancer is one of the most common cancers in Asia, and recently, various cases of resistance to fluorouracil treatment have been reported. In this study, we investigated the role of alternatively activated macrophages in the resistance of AGS gastric cancer cells to fluorouracil. THP-1 cells were polarized using recombinant human IL-4, then were cocultured with AGS cells treated with fluorouracil. Cell viability, Western blot, immunofluorescence, and cell invasion were performed for this investigation. Our results demonstrated that polarized macrophages initiated the survival of treated AGS cells and induced the resistance in AGS by upregulating the expression of integrin β3, focal adhesion protein (FAK), and cofilin proteins. These results reveal that integrin β3, focal adhesion protein (FAK), and cofilin proteins are potential targets for the improvement of fluorouracil efficacy in gastric cancer treatment.http://dx.doi.org/10.1155/2020/1731457 |
spellingShingle | Daniel Ngabire Irvine Niyonizigiye Maheshkumar Prakash Patil Yeong-Ae Seong Yong Bae Seo Gun-Do Kim M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin Journal of Immunology Research |
title | M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin |
title_full | M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin |
title_fullStr | M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin |
title_full_unstemmed | M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin |
title_short | M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin |
title_sort | m2 macrophages mediate the resistance of gastric adenocarcinoma cells to 5 fluorouracil through the expression of integrin β3 focal adhesion kinase and cofilin |
url | http://dx.doi.org/10.1155/2020/1731457 |
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