Driver lines for studying associative learning in Drosophila
The mushroom body (MB) is the center for associative learning in insects. In Drosophila, intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid the foundation for precise interrogation of the MB neural circuits. However, investigation of many cell types upstream and dow...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2025-01-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/94168 |
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| author | Yichun Shuai Megan Sammons Gabriella R Sterne Karen L Hibbard He Yang Ching-Po Yang Claire Managan Igor Siwanowicz Tzumin Lee Gerald M Rubin Glenn C Turner Yoshinori Aso |
| author_facet | Yichun Shuai Megan Sammons Gabriella R Sterne Karen L Hibbard He Yang Ching-Po Yang Claire Managan Igor Siwanowicz Tzumin Lee Gerald M Rubin Glenn C Turner Yoshinori Aso |
| author_sort | Yichun Shuai |
| collection | DOAJ |
| description | The mushroom body (MB) is the center for associative learning in insects. In Drosophila, intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid the foundation for precise interrogation of the MB neural circuits. However, investigation of many cell types upstream and downstream of the MB has been hindered due to lack of specific driver lines. Here we describe a new collection of over 800 split-GAL4 and split-LexA drivers that cover approximately 300 cell types, including sugar sensory neurons, putative nociceptive ascending neurons, olfactory and thermo-/hygro-sensory projection neurons, interneurons connected with the MB-extrinsic neurons, and various other cell types. We characterized activation phenotypes for a subset of these lines and identified a sugar sensory neuron line most suitable for reward substitution. Leveraging the thousands of confocal microscopy images associated with the collection, we analyzed neuronal morphological stereotypy and discovered that one set of mushroom body output neurons, MBON08/MBON09, exhibits striking individuality and asymmetry across animals. In conjunction with the EM connectome maps, the driver lines reported here offer a powerful resource for functional dissection of neural circuits for associative learning in adult Drosophila. |
| format | Article |
| id | doaj-art-0fd41ec94b1243419e2fe68effc86595 |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-0fd41ec94b1243419e2fe68effc865952025-01-29T17:14:01ZengeLife Sciences Publications LtdeLife2050-084X2025-01-011310.7554/eLife.94168Driver lines for studying associative learning in DrosophilaYichun Shuai0https://orcid.org/0000-0001-9243-425XMegan Sammons1Gabriella R Sterne2Karen L Hibbard3https://orcid.org/0000-0002-2001-6099He Yang4Ching-Po Yang5Claire Managan6Igor Siwanowicz7https://orcid.org/0000-0001-5819-1530Tzumin Lee8https://orcid.org/0000-0003-0569-0111Gerald M Rubin9https://orcid.org/0000-0001-8762-8703Glenn C Turner10https://orcid.org/0000-0002-5341-2784Yoshinori Aso11https://orcid.org/0000-0002-2939-1688Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, United StatesThe mushroom body (MB) is the center for associative learning in insects. In Drosophila, intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid the foundation for precise interrogation of the MB neural circuits. However, investigation of many cell types upstream and downstream of the MB has been hindered due to lack of specific driver lines. Here we describe a new collection of over 800 split-GAL4 and split-LexA drivers that cover approximately 300 cell types, including sugar sensory neurons, putative nociceptive ascending neurons, olfactory and thermo-/hygro-sensory projection neurons, interneurons connected with the MB-extrinsic neurons, and various other cell types. We characterized activation phenotypes for a subset of these lines and identified a sugar sensory neuron line most suitable for reward substitution. Leveraging the thousands of confocal microscopy images associated with the collection, we analyzed neuronal morphological stereotypy and discovered that one set of mushroom body output neurons, MBON08/MBON09, exhibits striking individuality and asymmetry across animals. In conjunction with the EM connectome maps, the driver lines reported here offer a powerful resource for functional dissection of neural circuits for associative learning in adult Drosophila.https://elifesciences.org/articles/94168learningdriversdopaminemushroom bodyindividuality |
| spellingShingle | Yichun Shuai Megan Sammons Gabriella R Sterne Karen L Hibbard He Yang Ching-Po Yang Claire Managan Igor Siwanowicz Tzumin Lee Gerald M Rubin Glenn C Turner Yoshinori Aso Driver lines for studying associative learning in Drosophila eLife learning drivers dopamine mushroom body individuality |
| title | Driver lines for studying associative learning in Drosophila |
| title_full | Driver lines for studying associative learning in Drosophila |
| title_fullStr | Driver lines for studying associative learning in Drosophila |
| title_full_unstemmed | Driver lines for studying associative learning in Drosophila |
| title_short | Driver lines for studying associative learning in Drosophila |
| title_sort | driver lines for studying associative learning in drosophila |
| topic | learning drivers dopamine mushroom body individuality |
| url | https://elifesciences.org/articles/94168 |
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