Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae)
Brazil is one of the largest consumers of herbicides in the world, and glyphosate-based herbicides (e.g., Roundup<sup>®</sup>) are commonly applied in cropland. Among vertebrates, amphibians are especially susceptible to contamination due to their strong association with freshwater envir...
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2024-12-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/13/1/4 |
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| author | Mario Barbosa da Silva Junior Renan Nunes Costa Iuri Ribeiro Dias Yvonnick Le Pendu Mirco Solé Caio Vinícius de Mira-Mendes Victor Goyannes Dill Orrico |
| author_facet | Mario Barbosa da Silva Junior Renan Nunes Costa Iuri Ribeiro Dias Yvonnick Le Pendu Mirco Solé Caio Vinícius de Mira-Mendes Victor Goyannes Dill Orrico |
| author_sort | Mario Barbosa da Silva Junior |
| collection | DOAJ |
| description | Brazil is one of the largest consumers of herbicides in the world, and glyphosate-based herbicides (e.g., Roundup<sup>®</sup>) are commonly applied in cropland. Among vertebrates, amphibians are especially susceptible to contamination due to their strong association with freshwater environments. However, our knowledge of how these herbicides affect amphibians is still scarce, mainly regarding the impacts of commercial formulations. In this study, we experimentally evaluated the effects of Roundup Original DI<sup>®</sup>, a glyphosate-based herbicide, on tadpoles of <i>Physalaemus cicada</i> and <i>Physalaemus erikae</i>. Spawnings were collected in south and southern Bahia, transported to the laboratory, and kept until the tadpoles reached developmental stage 25. Tadpoles were acclimated for 24 h and subsequently exposed to increasing herbicide concentrations at acute and chronic levels to assess survival, lethal concentration (LC50 96 h), and morphological and ontogenetic responses. Contamination significantly decreased the survival of tadpoles of both species and affected the development of tadpoles of <i>P. erikae.</i> The estimated lethal concentration (LC50) after 96 h for <i>Physalaemus cicada</i> was 5.52 mg a.i./L, and <i>Physalaemus erikae</i> was 3.40 mg a.i./L. Also, at chronic concentrations, morphological changes were observed in the tadpoles of <i>P. erikae</i>, with the changes being mainly in the tadpoles’ tail and body shapes. Therefore, Roundup Original DI<sup>®</sup> is considered moderately toxic to both species. Our results extend the knowledge regarding the action of pesticides mainly for species of the genus <i>Physalaemus</i>, which is the best-known genus for glyphosate based-herbicide toxicity. |
| format | Article |
| id | doaj-art-0fc9012655cd4d65aab426f94883666d |
| institution | Kabale University |
| issn | 2305-6304 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj-art-0fc9012655cd4d65aab426f94883666d2025-01-24T13:50:54ZengMDPI AGToxics2305-63042024-12-01131410.3390/toxics13010004Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae)Mario Barbosa da Silva Junior0Renan Nunes Costa1Iuri Ribeiro Dias2Yvonnick Le Pendu3Mirco Solé4Caio Vinícius de Mira-Mendes5Victor Goyannes Dill Orrico6Tropical Herpetology Lab, Graduate Program in Zoology, Department of Biological Sciences, State University of Santa Cruz, Ilhéus 45662-900, BA, BrazilDepartment of Biological Sciences, State University of Minas Gerais, Carangola 36800-000, MG, BrazilTropical Herpetology Lab, Graduate Program in Zoology, Department of Biological Sciences, State University of Santa Cruz, Ilhéus 45662-900, BA, BrazilDepartment of Biological Sciences, State University of Santa Cruz, Ilhéus 45662-900, BA, BrazilTropical Herpetology Lab, Graduate Program in Zoology, Department of Biological Sciences, State University of Santa Cruz, Ilhéus 45662-900, BA, BrazilDepartment of Biology, State University of Maranhão, São Luís 65055-310, MA, BrazilTropical Herpetology Lab, Graduate Program in Zoology, Department of Biological Sciences, State University of Santa Cruz, Ilhéus 45662-900, BA, BrazilBrazil is one of the largest consumers of herbicides in the world, and glyphosate-based herbicides (e.g., Roundup<sup>®</sup>) are commonly applied in cropland. Among vertebrates, amphibians are especially susceptible to contamination due to their strong association with freshwater environments. However, our knowledge of how these herbicides affect amphibians is still scarce, mainly regarding the impacts of commercial formulations. In this study, we experimentally evaluated the effects of Roundup Original DI<sup>®</sup>, a glyphosate-based herbicide, on tadpoles of <i>Physalaemus cicada</i> and <i>Physalaemus erikae</i>. Spawnings were collected in south and southern Bahia, transported to the laboratory, and kept until the tadpoles reached developmental stage 25. Tadpoles were acclimated for 24 h and subsequently exposed to increasing herbicide concentrations at acute and chronic levels to assess survival, lethal concentration (LC50 96 h), and morphological and ontogenetic responses. Contamination significantly decreased the survival of tadpoles of both species and affected the development of tadpoles of <i>P. erikae.</i> The estimated lethal concentration (LC50) after 96 h for <i>Physalaemus cicada</i> was 5.52 mg a.i./L, and <i>Physalaemus erikae</i> was 3.40 mg a.i./L. Also, at chronic concentrations, morphological changes were observed in the tadpoles of <i>P. erikae</i>, with the changes being mainly in the tadpoles’ tail and body shapes. Therefore, Roundup Original DI<sup>®</sup> is considered moderately toxic to both species. Our results extend the knowledge regarding the action of pesticides mainly for species of the genus <i>Physalaemus</i>, which is the best-known genus for glyphosate based-herbicide toxicity.https://www.mdpi.com/2305-6304/13/1/4survivalLC50acute exposure experienceglyphosatechronic exposure experience |
| spellingShingle | Mario Barbosa da Silva Junior Renan Nunes Costa Iuri Ribeiro Dias Yvonnick Le Pendu Mirco Solé Caio Vinícius de Mira-Mendes Victor Goyannes Dill Orrico Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae) Toxics survival LC50 acute exposure experience glyphosate chronic exposure experience |
| title | Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae) |
| title_full | Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae) |
| title_fullStr | Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae) |
| title_full_unstemmed | Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae) |
| title_short | Toxicity of the Herbicide Roundup Original DI<sup>®</sup> in Tadpoles of <i>Physalaemus erikae</i> and <i>Physalaemus cicada</i> (Anura: Leptodactylidae) |
| title_sort | toxicity of the herbicide roundup original di sup r sup in tadpoles of i physalaemus erikae i and i physalaemus cicada i anura leptodactylidae |
| topic | survival LC50 acute exposure experience glyphosate chronic exposure experience |
| url | https://www.mdpi.com/2305-6304/13/1/4 |
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