Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II)
Introduction: Acetic acid (AA) has been commonly used in medicine as an antiseptic agent for the past 6000 years. This study evaluated the antibacterial effect of AA during an outbreak in an intensive care unit (ICU) facility in Baja California Sur, México. Methodology: Thirty-five environmental...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2021-08-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/11693 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850034328368578560 |
|---|---|
| author | Carolina Garciglia-Mercado Ramon Gaxiola-Robles Felipe Ascencio Concepción Grajales-Muñiz Maria Luisa Soriano Rodríguez Jesus Silva-Sanchez Maria Teresa Estrada-Garcia Gracia A Gómez-Anduro |
| author_facet | Carolina Garciglia-Mercado Ramon Gaxiola-Robles Felipe Ascencio Concepción Grajales-Muñiz Maria Luisa Soriano Rodríguez Jesus Silva-Sanchez Maria Teresa Estrada-Garcia Gracia A Gómez-Anduro |
| author_sort | Carolina Garciglia-Mercado |
| collection | DOAJ |
| description |
Introduction: Acetic acid (AA) has been commonly used in medicine as an antiseptic agent for the past 6000 years. This study evaluated the antibacterial effect of AA during an outbreak in an intensive care unit (ICU) facility in Baja California Sur, México.
Methodology: Thirty-five environmental samples were collected, subsequently, disinfection with AA (4%) was performed, and two days later the same areas were sampled inside the ICU facility. Carbapenem-resistant A. baumannii (CRAB) was detected with loop-mediated isothermal amplification assay (Garciglia-Mercado et al. companion paper), targeting blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, blaOXA-58-like, blaIMP and blaVIM genes. CRAB isolates before and after disinfection were compared by PFGE.
Results: Eighteen (54.5%) and five (14.3%) of thirty-five environmental samples were identified as Acinetobacter baumannii before and after disinfection, respectively, showing a significant decrease of 85.7% (p < 0.05) both by Loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR). Furthermore, the presence of blaOXA-23-like and blaOXA-58-like genes significantly decreased (p < 0.05) both by LAMP and PCR methods. PFGE genotype showed high similarity among CRAB isolates before and after disinfection, suggesting wide clonal dissemination in the ICU facility.
Conclusions: This study demonstrated the novel application of AA with the LAMP assays developed for detecting CRAB. AA promises to be a cheap and efficacious disinfectant alternative to both developed and especially developing countries, preventing the spread of this organism in the environment and to other susceptible patients in health care settings.
|
| format | Article |
| id | doaj-art-0faf1a4c53624a249b22986d390c1106 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-0faf1a4c53624a249b22986d390c11062025-08-20T02:57:52ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-08-01150810.3855/jidc.11693Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II)Carolina Garciglia-Mercado0Ramon Gaxiola-Robles1Felipe Ascencio2Concepción Grajales-Muñiz3Maria Luisa Soriano Rodríguez4Jesus Silva-Sanchez5Maria Teresa Estrada-Garcia6Gracia A Gómez-Anduro7Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, MéxicoCentro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, MéxicoCentro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, MéxicoCoordinación de Vigilancia Epidemiológica en la División de Vigilancia Epidemiológica de Enfermedades Transmisibles, Instituto Mexicano del Seguro Social, CDMX, MéxicoUnidad Médica de Atención Ambulatoria (UMAA) con medicina familiar, 180 Delegación México oriente, Instituto Mexicano del Seguro Social, CDMX, MéxicoInstituto Nacional de Salud Pública, Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, MéxicoCINVESTAV-IPN, Departamento de Biomedicina Molecular, CDMX, MéxicoCentro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur, México Introduction: Acetic acid (AA) has been commonly used in medicine as an antiseptic agent for the past 6000 years. This study evaluated the antibacterial effect of AA during an outbreak in an intensive care unit (ICU) facility in Baja California Sur, México. Methodology: Thirty-five environmental samples were collected, subsequently, disinfection with AA (4%) was performed, and two days later the same areas were sampled inside the ICU facility. Carbapenem-resistant A. baumannii (CRAB) was detected with loop-mediated isothermal amplification assay (Garciglia-Mercado et al. companion paper), targeting blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, blaOXA-58-like, blaIMP and blaVIM genes. CRAB isolates before and after disinfection were compared by PFGE. Results: Eighteen (54.5%) and five (14.3%) of thirty-five environmental samples were identified as Acinetobacter baumannii before and after disinfection, respectively, showing a significant decrease of 85.7% (p < 0.05) both by Loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR). Furthermore, the presence of blaOXA-23-like and blaOXA-58-like genes significantly decreased (p < 0.05) both by LAMP and PCR methods. PFGE genotype showed high similarity among CRAB isolates before and after disinfection, suggesting wide clonal dissemination in the ICU facility. Conclusions: This study demonstrated the novel application of AA with the LAMP assays developed for detecting CRAB. AA promises to be a cheap and efficacious disinfectant alternative to both developed and especially developing countries, preventing the spread of this organism in the environment and to other susceptible patients in health care settings. https://jidc.org/index.php/journal/article/view/11693Acetic acidCarbapenem-resistanceAcinetobacter baumanniiloop-mediated isothermal amplification |
| spellingShingle | Carolina Garciglia-Mercado Ramon Gaxiola-Robles Felipe Ascencio Concepción Grajales-Muñiz Maria Luisa Soriano Rodríguez Jesus Silva-Sanchez Maria Teresa Estrada-Garcia Gracia A Gómez-Anduro Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II) Journal of Infection in Developing Countries Acetic acid Carbapenem-resistance Acinetobacter baumannii loop-mediated isothermal amplification |
| title | Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II) |
| title_full | Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II) |
| title_fullStr | Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II) |
| title_full_unstemmed | Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II) |
| title_short | Antibacterial effect of acetic acid during an outbreak of carbapenem-resistant Acinetobacter baumannii in an ICU (II) |
| title_sort | antibacterial effect of acetic acid during an outbreak of carbapenem resistant acinetobacter baumannii in an icu ii |
| topic | Acetic acid Carbapenem-resistance Acinetobacter baumannii loop-mediated isothermal amplification |
| url | https://jidc.org/index.php/journal/article/view/11693 |
| work_keys_str_mv | AT carolinagarcigliamercado antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii AT ramongaxiolarobles antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii AT felipeascencio antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii AT concepciongrajalesmuniz antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii AT marialuisasorianorodriguez antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii AT jesussilvasanchez antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii AT mariateresaestradagarcia antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii AT graciaagomezanduro antibacterialeffectofaceticacidduringanoutbreakofcarbapenemresistantacinetobacterbaumanniiinanicuii |