RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression
RNA binding proteins (RBPs) play crucial roles in the post-transcriptional regulation of metabolic pathways. Although the RBP HuD has been extensively studied in pancreatic β-cells, its role in cellular metabolism remains poorly understood. In this study, we uncover a novel function of HuD in regula...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2025.2542168 |
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| author | Jiyoon Seo Seungyeon Ryu Wei Zhang Eun Kyung Lee Seung Min Jeong |
| author_facet | Jiyoon Seo Seungyeon Ryu Wei Zhang Eun Kyung Lee Seung Min Jeong |
| author_sort | Jiyoon Seo |
| collection | DOAJ |
| description | RNA binding proteins (RBPs) play crucial roles in the post-transcriptional regulation of metabolic pathways. Although the RBP HuD has been extensively studied in pancreatic β-cells, its role in cellular metabolism remains poorly understood. In this study, we uncover a novel function of HuD in regulating fatty acid oxidation (FAO) in mouse insulinoma βTC6 cells. Through genetic knockdown and overexpression approaches, we demonstrate that HuD modulates the expression of long-chain acyl-CoA dehydrogenase (LCAD), a key enzyme in FAO, by binding to the 3′-untranslated region of its mRNA. Loss of HuD impaired FAO, leading to lipid droplet accumulation, elevated reactive oxygen species production, and increased lipotoxicity under lipid-stress conditions. These findings reveal a previously unrecognized role for HuD in maintaining fatty acid homeostasis and suggest that the HuD-LCAD regulatory axis may represent a promising therapeutic target for preserving β-cell integrity and function. |
| format | Article |
| id | doaj-art-0fac34e0e15f4a93b0f13c7cd0887fd9 |
| institution | Kabale University |
| issn | 1976-8354 2151-2485 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj-art-0fac34e0e15f4a93b0f13c7cd0887fd92025-08-20T03:40:41ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852025-12-0129151252210.1080/19768354.2025.2542168RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expressionJiyoon Seo0Seungyeon Ryu1Wei Zhang2Eun Kyung Lee3Seung Min Jeong4Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaShanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaDepartment of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, Republic of KoreaRNA binding proteins (RBPs) play crucial roles in the post-transcriptional regulation of metabolic pathways. Although the RBP HuD has been extensively studied in pancreatic β-cells, its role in cellular metabolism remains poorly understood. In this study, we uncover a novel function of HuD in regulating fatty acid oxidation (FAO) in mouse insulinoma βTC6 cells. Through genetic knockdown and overexpression approaches, we demonstrate that HuD modulates the expression of long-chain acyl-CoA dehydrogenase (LCAD), a key enzyme in FAO, by binding to the 3′-untranslated region of its mRNA. Loss of HuD impaired FAO, leading to lipid droplet accumulation, elevated reactive oxygen species production, and increased lipotoxicity under lipid-stress conditions. These findings reveal a previously unrecognized role for HuD in maintaining fatty acid homeostasis and suggest that the HuD-LCAD regulatory axis may represent a promising therapeutic target for preserving β-cell integrity and function.https://www.tandfonline.com/doi/10.1080/19768354.2025.2542168HuDLCADfatty acid oxidationlipotoxicitypancreatic β-cell |
| spellingShingle | Jiyoon Seo Seungyeon Ryu Wei Zhang Eun Kyung Lee Seung Min Jeong RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression Animal Cells and Systems HuD LCAD fatty acid oxidation lipotoxicity pancreatic β-cell |
| title | RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression |
| title_full | RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression |
| title_fullStr | RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression |
| title_full_unstemmed | RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression |
| title_short | RNA binding protein HuD regulates fatty acid oxidation in pancreatic β-cells by modulating long-chain acyl-CoA dehydrogenase expression |
| title_sort | rna binding protein hud regulates fatty acid oxidation in pancreatic β cells by modulating long chain acyl coa dehydrogenase expression |
| topic | HuD LCAD fatty acid oxidation lipotoxicity pancreatic β-cell |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2025.2542168 |
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