Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos
Dimethomorph (DMT) is a widely-used selective active fungicide that effectively controls downy mildew, crown rot, and late blight in crops. The extensive application of DMT raises concerns about its ecological impact on non-target organisms in the environment. However, there is limited understanding...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324014891 |
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| author | You Wei Yunlong Meng Kun Jia Weijian Lu Yushan Huang Huiqiang Lu |
| author_facet | You Wei Yunlong Meng Kun Jia Weijian Lu Yushan Huang Huiqiang Lu |
| author_sort | You Wei |
| collection | DOAJ |
| description | Dimethomorph (DMT) is a widely-used selective active fungicide that effectively controls downy mildew, crown rot, and late blight in crops. The extensive application of DMT raises concerns about its ecological impact on non-target organisms in the environment. However, there is limited understanding of the toxicological properties of DMT on these organisms. In this study, we utilized zebrafish as an animal model to assess the toxicity of DMT induced by exposure 5.5–72 hours post-fertilization (hpf). During this period, we monitored and evaluated the development of the zebrafish heart and vascular system. Additionally, embryo samples were collected to perform molecular-level detection of PCNA, oxidative stress, and related genes. The results showed a concentration-dependent decrease in survival rate and hatching rate, shortened body length, slowed heart rate, and pericardial edema, body curvature and reduced eye size as DMT exposure concentration increased. Furthermore, DMT exposure led to impairments in the development of the heart, vascular, along with change in the expression levels of relevant genes. It also caused a decrease in cell proliferation and an increase in oxidative stress levels. Moreover, DMT disrupts the normal development of thyroid follicular cells, leading to a reduction in T3 levels. Thyroid hormone supplementation partially reverses the toxicity induced by DMT, increasing eye size, restoring body length, reducing spine curvature, and reducing pericardial edema. Therefore, we speculate that DMT likely affects the development of zebrafish embryos by disrupting normal thyroid follicle development. |
| format | Article |
| id | doaj-art-0f7b235a2a94444cabb91e58b9c3980e |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-0f7b235a2a94444cabb91e58b9c3980e2025-01-23T05:25:27ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117413Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryosYou Wei0Yunlong Meng1Kun Jia2Weijian Lu3Yushan Huang4Huiqiang Lu5Center for Clinical Medicine Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China; Center for drug Screening and Research, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou, Jiangxi 341000, ChinaCenter for Clinical Medicine Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China; Center for drug Screening and Research, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou, Jiangxi 341000, ChinaCenter for drug Screening and Research, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou, Jiangxi 341000, ChinaCenter for Clinical Medicine Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaCenter for Evidence Based Medical and Clinical Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, China; Correspondence to: Center for Evidence Based Medical and Clinical Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China.Center for Clinical Medicine Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China; Correspondence to: Center for Clinical Medicine Research, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi 341000, China.Dimethomorph (DMT) is a widely-used selective active fungicide that effectively controls downy mildew, crown rot, and late blight in crops. The extensive application of DMT raises concerns about its ecological impact on non-target organisms in the environment. However, there is limited understanding of the toxicological properties of DMT on these organisms. In this study, we utilized zebrafish as an animal model to assess the toxicity of DMT induced by exposure 5.5–72 hours post-fertilization (hpf). During this period, we monitored and evaluated the development of the zebrafish heart and vascular system. Additionally, embryo samples were collected to perform molecular-level detection of PCNA, oxidative stress, and related genes. The results showed a concentration-dependent decrease in survival rate and hatching rate, shortened body length, slowed heart rate, and pericardial edema, body curvature and reduced eye size as DMT exposure concentration increased. Furthermore, DMT exposure led to impairments in the development of the heart, vascular, along with change in the expression levels of relevant genes. It also caused a decrease in cell proliferation and an increase in oxidative stress levels. Moreover, DMT disrupts the normal development of thyroid follicular cells, leading to a reduction in T3 levels. Thyroid hormone supplementation partially reverses the toxicity induced by DMT, increasing eye size, restoring body length, reducing spine curvature, and reducing pericardial edema. Therefore, we speculate that DMT likely affects the development of zebrafish embryos by disrupting normal thyroid follicle development.http://www.sciencedirect.com/science/article/pii/S0147651324014891DimethomorphZebrafishThyroid folliclesOxidative stress |
| spellingShingle | You Wei Yunlong Meng Kun Jia Weijian Lu Yushan Huang Huiqiang Lu Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos Ecotoxicology and Environmental Safety Dimethomorph Zebrafish Thyroid follicles Oxidative stress |
| title | Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos |
| title_full | Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos |
| title_fullStr | Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos |
| title_full_unstemmed | Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos |
| title_short | Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos |
| title_sort | dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos |
| topic | Dimethomorph Zebrafish Thyroid follicles Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324014891 |
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