Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22
Abstract Laccases with high catalytic efficiency and high thermostability can drive a broader application scope. However, the structural distribution of key amino acids capable of significantly influencing the performance of laccases has not been explored in depth. Thirty laccase 13B22 mutants with...
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SpringerOpen
2025-05-01
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| Series: | Bioresources and Bioprocessing |
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| Online Access: | https://doi.org/10.1186/s40643-025-00893-6 |
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| author | Ruohan Zhang Yuchen Wang Xiaolu Wang Huiying Luo Yuan Wang Bin Yao Huoqing Huang Jian Tian Feifei Guan |
| author_facet | Ruohan Zhang Yuchen Wang Xiaolu Wang Huiying Luo Yuan Wang Bin Yao Huoqing Huang Jian Tian Feifei Guan |
| author_sort | Ruohan Zhang |
| collection | DOAJ |
| description | Abstract Laccases with high catalytic efficiency and high thermostability can drive a broader application scope. However, the structural distribution of key amino acids capable of significantly influencing the performance of laccases has not been explored in depth. Thirty laccase 13B22 mutants with changes in amino acids at distances of 5 Å (first shell), 5–8 Å (second shell), and 8–12 Å (third shell) from the active center were validated experimentally with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as substrate. Twelve of these mutants (first shell, 1; second shell, 4; third shell, 7) showed higher catalytic efficiency than the wild-type enzyme. Mutants D511E and I88L-D511E showed 5.36- and 10.58-fold increases in k cat/K m, respectively, with increases in optimal temperature of 15 °C and optimal pH from 7.0 to 8.0. Furthermore, both mutants exhibited greater thermostability compared to the wild-type, with increases of 3.33 °C and 5.06 °C in T m and decreases of 0.39 J and 0.59 J in total structure energy, respectively. The D511E mutation resides in the third shell, while I88L is in the second shell, and their performance enhancements were attributed to alterations in the rigidity or flexibility of specific protein structural domains. Both mutants showed enhanced degradation efficiency for benzo[a]pyrene and zearalenone. These findings highlight the importance of the residues located far from the active center in the function of laccase (second shell and third shell), suggesting broader implications for enzyme optimization and biotechnological applications. |
| format | Article |
| id | doaj-art-0f75671dfd434e809b901bba07b149f3 |
| institution | OA Journals |
| issn | 2197-4365 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SpringerOpen |
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| series | Bioresources and Bioprocessing |
| spelling | doaj-art-0f75671dfd434e809b901bba07b149f32025-08-20T02:00:00ZengSpringerOpenBioresources and Bioprocessing2197-43652025-05-0112111210.1186/s40643-025-00893-6Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22Ruohan Zhang0Yuchen Wang1Xiaolu Wang2Huiying Luo3Yuan Wang4Bin Yao5Huoqing Huang6Jian Tian7Feifei Guan8State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesNational Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural SciencesNational Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural SciencesAbstract Laccases with high catalytic efficiency and high thermostability can drive a broader application scope. However, the structural distribution of key amino acids capable of significantly influencing the performance of laccases has not been explored in depth. Thirty laccase 13B22 mutants with changes in amino acids at distances of 5 Å (first shell), 5–8 Å (second shell), and 8–12 Å (third shell) from the active center were validated experimentally with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as substrate. Twelve of these mutants (first shell, 1; second shell, 4; third shell, 7) showed higher catalytic efficiency than the wild-type enzyme. Mutants D511E and I88L-D511E showed 5.36- and 10.58-fold increases in k cat/K m, respectively, with increases in optimal temperature of 15 °C and optimal pH from 7.0 to 8.0. Furthermore, both mutants exhibited greater thermostability compared to the wild-type, with increases of 3.33 °C and 5.06 °C in T m and decreases of 0.39 J and 0.59 J in total structure energy, respectively. The D511E mutation resides in the third shell, while I88L is in the second shell, and their performance enhancements were attributed to alterations in the rigidity or flexibility of specific protein structural domains. Both mutants showed enhanced degradation efficiency for benzo[a]pyrene and zearalenone. These findings highlight the importance of the residues located far from the active center in the function of laccase (second shell and third shell), suggesting broader implications for enzyme optimization and biotechnological applications.https://doi.org/10.1186/s40643-025-00893-6LaccaseCatalytic activityStructural distanceMutantThermostability |
| spellingShingle | Ruohan Zhang Yuchen Wang Xiaolu Wang Huiying Luo Yuan Wang Bin Yao Huoqing Huang Jian Tian Feifei Guan Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22 Bioresources and Bioprocessing Laccase Catalytic activity Structural distance Mutant Thermostability |
| title | Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22 |
| title_full | Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22 |
| title_fullStr | Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22 |
| title_full_unstemmed | Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22 |
| title_short | Influence of mutations at different distances from the active center on the activity and stability of laccase 13B22 |
| title_sort | influence of mutations at different distances from the active center on the activity and stability of laccase 13b22 |
| topic | Laccase Catalytic activity Structural distance Mutant Thermostability |
| url | https://doi.org/10.1186/s40643-025-00893-6 |
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