Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis
Subacute Sclerosing Panencephalitis (SSPE), a rare lethal disease of children and young adults due to persistence of measles virus (MeV) in the brain, is caused by wild type (wt) MeV. Why MeV vaccine strains never cause SSPE is completely unknown. Hypothesizing that this phenotypic difference could...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2015/769837 |
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| author | Hasan Kweder Michelle Ainouze Joanna Brunel Denis Gerlier Evelyne Manet Robin Buckland |
| author_facet | Hasan Kweder Michelle Ainouze Joanna Brunel Denis Gerlier Evelyne Manet Robin Buckland |
| author_sort | Hasan Kweder |
| collection | DOAJ |
| description | Subacute Sclerosing Panencephalitis (SSPE), a rare lethal disease of children and young adults due to persistence of measles virus (MeV) in the brain, is caused by wild type (wt) MeV. Why MeV vaccine strains never cause SSPE is completely unknown. Hypothesizing that this phenotypic difference could potentially be represented by a molecular marker, we compared glycoprotein and matrix (M) genes from SSPE cases with those from the Moraten vaccine strain, searching for differential structural motifs. We observed that all known SSPE viruses have residues P64, E89, and A209 (PEA) in their M proteins whereas the equivalent residues for vaccine strains are either S64, K89, and T209 (SKT) as in Moraten or PKT. Through the construction of MeV recombinants, we have obtained evidence that the wt MeV-M protein PEA motif, in particular A209, is linked to increased viral spread. Importantly, for the 10 wt genotypes (of 23) that have had their M proteins sequenced, 9 have the PEA motif, the exception being B3, which has PET. Interestingly, cases of SSPE caused by genotype B3 have yet to be reported. In conclusion, our results strongly suggest that the PEA motif is a molecular marker for wt MeV at risk to cause SSPE. |
| format | Article |
| id | doaj-art-0f60ff328b434ef0b770237889f31a93 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-0f60ff328b434ef0b770237889f31a932025-02-03T06:46:42ZengWileyAdvances in Virology1687-86391687-86472015-01-01201510.1155/2015/769837769837Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing PanencephalitisHasan Kweder0Michelle Ainouze1Joanna Brunel2Denis Gerlier3Evelyne Manet4Robin Buckland5CIRI, International Center for Infectiology Research, Université de Lyon, 69007 Lyon, FranceCIRI, International Center for Infectiology Research, Université de Lyon, 69007 Lyon, FranceCIRI, International Center for Infectiology Research, Université de Lyon, 69007 Lyon, FranceCIRI, International Center for Infectiology Research, Université de Lyon, 69007 Lyon, FranceCIRI, International Center for Infectiology Research, Université de Lyon, 69007 Lyon, FranceCIRI, International Center for Infectiology Research, Université de Lyon, 69007 Lyon, FranceSubacute Sclerosing Panencephalitis (SSPE), a rare lethal disease of children and young adults due to persistence of measles virus (MeV) in the brain, is caused by wild type (wt) MeV. Why MeV vaccine strains never cause SSPE is completely unknown. Hypothesizing that this phenotypic difference could potentially be represented by a molecular marker, we compared glycoprotein and matrix (M) genes from SSPE cases with those from the Moraten vaccine strain, searching for differential structural motifs. We observed that all known SSPE viruses have residues P64, E89, and A209 (PEA) in their M proteins whereas the equivalent residues for vaccine strains are either S64, K89, and T209 (SKT) as in Moraten or PKT. Through the construction of MeV recombinants, we have obtained evidence that the wt MeV-M protein PEA motif, in particular A209, is linked to increased viral spread. Importantly, for the 10 wt genotypes (of 23) that have had their M proteins sequenced, 9 have the PEA motif, the exception being B3, which has PET. Interestingly, cases of SSPE caused by genotype B3 have yet to be reported. In conclusion, our results strongly suggest that the PEA motif is a molecular marker for wt MeV at risk to cause SSPE.http://dx.doi.org/10.1155/2015/769837 |
| spellingShingle | Hasan Kweder Michelle Ainouze Joanna Brunel Denis Gerlier Evelyne Manet Robin Buckland Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis Advances in Virology |
| title | Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis |
| title_full | Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis |
| title_fullStr | Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis |
| title_full_unstemmed | Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis |
| title_short | Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis |
| title_sort | measles virus identification in the m protein primary sequence of a potential molecular marker for subacute sclerosing panencephalitis |
| url | http://dx.doi.org/10.1155/2015/769837 |
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