Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates
The pathogen "Pseudomonas aeruginosa" is extremely hazardous for people with weak immune systems because it has several virulence factors and can resist antibiotics. During the time period between October 2022 and February 2023, 102 specimens from different clinical sources, including bur...
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University of Baghdad
2025-01-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
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| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3512 |
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| author | Zeinab Waheed kadim Hala Mouayed Radif |
| author_facet | Zeinab Waheed kadim Hala Mouayed Radif |
| author_sort | Zeinab Waheed kadim |
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The pathogen "Pseudomonas aeruginosa" is extremely hazardous for people with weak immune systems because it has several virulence factors and can resist antibiotics. During the time period between October 2022 and February 2023, 102 specimens from different clinical sources, including burn, mid-stream urine, wound, ear, and sputum were collected. From 102 clinical specimens, only 33 isolates (32.35%) of P. aeruginosa were identified by Phenotypic characteristics, a biochemical test, the Vitek-2 compact system, and confirmed by Conventional Polymerase Chain Reaction (PCR). The antibiotic susceptibility test AST was done by the Vitek 2-Compact system. The highest resistance percentages were for Cephalosporin category at 81.8% (Cefotaxime, Ceftazidime, and Cefepime), while the lowest percentage was for the Fluoroquinolone category at 42.4% (Ciprofloxacin and Norfloxacin); 18 (54.5%) of the isolates were categorized as MDR. The production rates of virulence factors investigated in all tested isolates were 100% for protease, hemolysin, and swarming, while the lipase production rate was 48.5%.
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| format | Article |
| id | doaj-art-0f4c82c5cc784fd4a937168d80c37896 |
| institution | Kabale University |
| issn | 1609-4042 2521-3407 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj-art-0f4c82c5cc784fd4a937168d80c378962025-01-22T01:21:08ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072025-01-0138110.30526/38.1.3512Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolatesZeinab Waheed kadim0https://orcid.org/0009-0003-5224-7339Hala Mouayed Radif1https://orcid.org/0000-0002-6143-1854Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. The pathogen "Pseudomonas aeruginosa" is extremely hazardous for people with weak immune systems because it has several virulence factors and can resist antibiotics. During the time period between October 2022 and February 2023, 102 specimens from different clinical sources, including burn, mid-stream urine, wound, ear, and sputum were collected. From 102 clinical specimens, only 33 isolates (32.35%) of P. aeruginosa were identified by Phenotypic characteristics, a biochemical test, the Vitek-2 compact system, and confirmed by Conventional Polymerase Chain Reaction (PCR). The antibiotic susceptibility test AST was done by the Vitek 2-Compact system. The highest resistance percentages were for Cephalosporin category at 81.8% (Cefotaxime, Ceftazidime, and Cefepime), while the lowest percentage was for the Fluoroquinolone category at 42.4% (Ciprofloxacin and Norfloxacin); 18 (54.5%) of the isolates were categorized as MDR. The production rates of virulence factors investigated in all tested isolates were 100% for protease, hemolysin, and swarming, while the lipase production rate was 48.5%. https://jih.uobaghdad.edu.iq/index.php/j/article/view/3512Proteasehemolysinswarming lipaseantibiotic patterns |
| spellingShingle | Zeinab Waheed kadim Hala Mouayed Radif Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates Ibn Al-Haitham Journal for Pure and Applied Sciences Protease hemolysin swarming lipase antibiotic patterns |
| title | Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates |
| title_full | Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates |
| title_fullStr | Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates |
| title_full_unstemmed | Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates |
| title_short | Investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates |
| title_sort | investigation of virulence factors in resistance and sensitive pseudomonas aeruginosa clinical isolates |
| topic | Protease hemolysin swarming lipase antibiotic patterns |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3512 |
| work_keys_str_mv | AT zeinabwaheedkadim investigationofvirulencefactorsinresistanceandsensitivepseudomonasaeruginosaclinicalisolates AT halamouayedradif investigationofvirulencefactorsinresistanceandsensitivepseudomonasaeruginosaclinicalisolates |