Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants
Soybean lectin (SBL) purified from soybean seeds by affinity chromatography strongly bound to Bradyrhizobium japonicum USDA 110 cell surface. This lectin enhanced biofilm formation by B. japonicum in a concentration-dependent manner. Presence of galactose during biofilm formation had different effec...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2009/719367 |
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| author | Julieta Pérez-Giménez Elías J. Mongiardini M. Julia Althabegoiti Julieta Covelli J. Ignacio Quelas Silvina L. López-García Aníbal R. Lodeiro |
| author_facet | Julieta Pérez-Giménez Elías J. Mongiardini M. Julia Althabegoiti Julieta Covelli J. Ignacio Quelas Silvina L. López-García Aníbal R. Lodeiro |
| author_sort | Julieta Pérez-Giménez |
| collection | DOAJ |
| description | Soybean lectin (SBL) purified from soybean seeds by affinity chromatography strongly bound to Bradyrhizobium japonicum USDA 110 cell surface. This lectin enhanced biofilm formation by B. japonicum in a concentration-dependent manner. Presence of galactose during biofilm formation had different effects in the presence or absence of SBL. Biofilms were completely inhibited in the presence of both SBL and galactose, while in the absence of SBL, galactose was less inhibitory. SBL was very stable, since its agglutinating activity of B. japonicum cells as well as of human group A+ erythrocytes was resistant to preincubation for one week at 60°C. Hence, we propose that plant remnants might constitute a source of this lectin, which might remain active in soil and thus favor B. japonicum biofilm formation in the interval between soybean crop seasons. |
| format | Article |
| id | doaj-art-0f36480cb3ee41e2a013c13c45a48562 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-0f36480cb3ee41e2a013c13c45a485622025-02-03T01:26:36ZengWileyInternational Journal of Microbiology1687-918X1687-91982009-01-01200910.1155/2009/719367719367Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of PlantsJulieta Pérez-Giménez0Elías J. Mongiardini1M. Julia Althabegoiti2Julieta Covelli3J. Ignacio Quelas4Silvina L. López-García5Aníbal R. Lodeiro6Instituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, ArgentinaInstituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, ArgentinaInstituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, ArgentinaInstituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, ArgentinaInstituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, ArgentinaInstituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, ArgentinaInstituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Calles 47 y 115 (1900) La Plata, ArgentinaSoybean lectin (SBL) purified from soybean seeds by affinity chromatography strongly bound to Bradyrhizobium japonicum USDA 110 cell surface. This lectin enhanced biofilm formation by B. japonicum in a concentration-dependent manner. Presence of galactose during biofilm formation had different effects in the presence or absence of SBL. Biofilms were completely inhibited in the presence of both SBL and galactose, while in the absence of SBL, galactose was less inhibitory. SBL was very stable, since its agglutinating activity of B. japonicum cells as well as of human group A+ erythrocytes was resistant to preincubation for one week at 60°C. Hence, we propose that plant remnants might constitute a source of this lectin, which might remain active in soil and thus favor B. japonicum biofilm formation in the interval between soybean crop seasons.http://dx.doi.org/10.1155/2009/719367 |
| spellingShingle | Julieta Pérez-Giménez Elías J. Mongiardini M. Julia Althabegoiti Julieta Covelli J. Ignacio Quelas Silvina L. López-García Aníbal R. Lodeiro Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants International Journal of Microbiology |
| title | Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants |
| title_full | Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants |
| title_fullStr | Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants |
| title_full_unstemmed | Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants |
| title_short | Soybean Lectin Enhances Biofilm Formation by Bradyrhizobium japonicum in the Absence of Plants |
| title_sort | soybean lectin enhances biofilm formation by bradyrhizobium japonicum in the absence of plants |
| url | http://dx.doi.org/10.1155/2009/719367 |
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