NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer
Gene mutations play a critical role in cancer development and progression, and their identification offers possibilities for accurate diagnostics and therapeutic targeting. Finding genes undergoing mutations is challenging and slow, even in the post-genomic era. A new approach was recently developed...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2005/478316 |
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| _version_ | 1832564727737221120 |
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| author | Maija Wolf Henrik Edgren Aslaug Muggerud Sami Kilpinen Pia Huusko Therese Sørlie Spyro Mousses Olli Kallioniemi |
| author_facet | Maija Wolf Henrik Edgren Aslaug Muggerud Sami Kilpinen Pia Huusko Therese Sørlie Spyro Mousses Olli Kallioniemi |
| author_sort | Maija Wolf |
| collection | DOAJ |
| description | Gene mutations play a critical role in cancer development and progression, and their identification offers possibilities for accurate diagnostics and therapeutic targeting. Finding genes undergoing mutations is challenging and slow, even in the post-genomic era. A new approach was recently developed by Noensie and Dietz to prioritize and focus the search, making use of nonsense-mediated mRNA decay (NMD) inhibition and microarray analysis (NMD microarrays) in the identification of transcripts containing nonsense mutations. We combined NMD microarrays with array-based CGH (comparative genomic hybridization) in order to identify inactivation of tumor suppressor genes in cancer. Such a “mutatomics” screening of prostate cancer cell lines led to the identification of inactivating mutations in the EPHB2 gene. Up to 8% of metastatic uncultured prostate cancers also showed mutations of this gene whose loss of function may confer loss of tissue architecture. NMD microarray analysis could turn out to be a powerful research method to identify novel mutated genes in cancer cell lines, providing targets that could then be further investigated for their clinical relevance and therapeutic potential. |
| format | Article |
| id | doaj-art-0f22c64c81d7405987e6ba768500a099 |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-0f22c64c81d7405987e6ba768500a0992025-02-03T01:10:16ZengWileyCellular Oncology1570-58701875-86062005-01-0127316917310.1155/2005/478316NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in CancerMaija Wolf0Henrik Edgren1Aslaug Muggerud2Sami Kilpinen3Pia Huusko4Therese Sørlie5Spyro Mousses6Olli Kallioniemi7Medical Biotechnology, VTT Technical Research Centre of Finland and University of Turku, FIN-20520 Turku, FinlandMedical Biotechnology, VTT Technical Research Centre of Finland and University of Turku, FIN-20520 Turku, FinlandDepartment of Genetics, The Norwegian Radium Hospital, N-0310 Oslo, NorwayMedical Biotechnology, VTT Technical Research Centre of Finland and University of Turku, FIN-20520 Turku, FinlandTranslational Genomics Research Institute, Gaithersburg, MD 20878-1762, USADepartment of Genetics, The Norwegian Radium Hospital, N-0310 Oslo, NorwayTranslational Genomics Research Institute, Gaithersburg, MD 20878-1762, USAMedical Biotechnology, VTT Technical Research Centre of Finland and University of Turku, FIN-20520 Turku, FinlandGene mutations play a critical role in cancer development and progression, and their identification offers possibilities for accurate diagnostics and therapeutic targeting. Finding genes undergoing mutations is challenging and slow, even in the post-genomic era. A new approach was recently developed by Noensie and Dietz to prioritize and focus the search, making use of nonsense-mediated mRNA decay (NMD) inhibition and microarray analysis (NMD microarrays) in the identification of transcripts containing nonsense mutations. We combined NMD microarrays with array-based CGH (comparative genomic hybridization) in order to identify inactivation of tumor suppressor genes in cancer. Such a “mutatomics” screening of prostate cancer cell lines led to the identification of inactivating mutations in the EPHB2 gene. Up to 8% of metastatic uncultured prostate cancers also showed mutations of this gene whose loss of function may confer loss of tissue architecture. NMD microarray analysis could turn out to be a powerful research method to identify novel mutated genes in cancer cell lines, providing targets that could then be further investigated for their clinical relevance and therapeutic potential.http://dx.doi.org/10.1155/2005/478316 |
| spellingShingle | Maija Wolf Henrik Edgren Aslaug Muggerud Sami Kilpinen Pia Huusko Therese Sørlie Spyro Mousses Olli Kallioniemi NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer Cellular Oncology |
| title | NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer |
| title_full | NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer |
| title_fullStr | NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer |
| title_full_unstemmed | NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer |
| title_short | NMD Microarray Analysis for Rapid Genome-Wide Screen of Mutated Genes in Cancer |
| title_sort | nmd microarray analysis for rapid genome wide screen of mutated genes in cancer |
| url | http://dx.doi.org/10.1155/2005/478316 |
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