Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems
Antibiotic resistance poses a significant threat to human and animal health worldwide, with farms serving as crucial reservoirs of Antibiotic Resistance Genes (ARGs) and Antibiotic-resistant bacteria. However, the distribution of ARGs in poultry farms and their transmission patterns in the environme...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124012434 |
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| _version_ | 1832591819384291328 |
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| author | ShangMin Li Kai Wang DanPing Wang HongZhi Wang HuaXuan Zhao JunHua Pu KeHua Wang ChunMei Li |
| author_facet | ShangMin Li Kai Wang DanPing Wang HongZhi Wang HuaXuan Zhao JunHua Pu KeHua Wang ChunMei Li |
| author_sort | ShangMin Li |
| collection | DOAJ |
| description | Antibiotic resistance poses a significant threat to human and animal health worldwide, with farms serving as crucial reservoirs of Antibiotic Resistance Genes (ARGs) and Antibiotic-resistant bacteria. However, the distribution of ARGs in poultry farms and their transmission patterns in the environment remain poorly understood. This study collected samples of aerosol microorganisms, cloacal matter, soil, and vegetables from poultry farms and surrounding environments at three different distances. We used 16S rRNA gene sequencing and HT-qPCR to analyze the characteristics of aerosol microbial communities and the abundance of ARGs. At the phylum level, Proteobacteria, Firmicutes, and Bacteroidetes were dominant in cloacal samples, aerosol samples, and vegetable samples, while Proteobacteria Actinobacteriota and Acidobacteria dominated soil. Pseudomonas was dominant in cloacal samples at the genus level, whereas Fusobacterium was prevalent in soil. The diversity and richness of bacterial communities were more similar between cloacal samples than those observed between either sample type compared with soil. Our results showed that tetracycline and aminoglycoside ARG relative abundance was high across all sample types but significantly increased within feces/air compared to soils/vegetables. Association analysis revealed five potential host genera for ARG/MGE presence among various microbiota populations studied here. Our findings confirm that farms are important sources for the environmental dissemination of pathogens and ARGs. |
| format | Article |
| id | doaj-art-0ef5ba6ad73a4739a2c098b141a24c90 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-0ef5ba6ad73a4739a2c098b141a24c902025-01-22T05:40:52ZengElsevierPoultry Science0032-57912025-01-011041104665Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystemsShangMin Li0Kai Wang1DanPing Wang2HongZhi Wang3HuaXuan Zhao4JunHua Pu5KeHua Wang6ChunMei Li7College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China; Jiangsu Institute of Poultry Sciences, Yangzhou, 225125, ChinaCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, ChinaJiangsu Institute of Poultry Sciences, Yangzhou, 225125, ChinaJiangsu Institute of Poultry Sciences, Yangzhou, 225125, ChinaJiangsu Institute of Poultry Sciences, Yangzhou, 225125, ChinaJiangsu Institute of Poultry Sciences, Yangzhou, 225125, ChinaCollege of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China; Corresponding author.Antibiotic resistance poses a significant threat to human and animal health worldwide, with farms serving as crucial reservoirs of Antibiotic Resistance Genes (ARGs) and Antibiotic-resistant bacteria. However, the distribution of ARGs in poultry farms and their transmission patterns in the environment remain poorly understood. This study collected samples of aerosol microorganisms, cloacal matter, soil, and vegetables from poultry farms and surrounding environments at three different distances. We used 16S rRNA gene sequencing and HT-qPCR to analyze the characteristics of aerosol microbial communities and the abundance of ARGs. At the phylum level, Proteobacteria, Firmicutes, and Bacteroidetes were dominant in cloacal samples, aerosol samples, and vegetable samples, while Proteobacteria Actinobacteriota and Acidobacteria dominated soil. Pseudomonas was dominant in cloacal samples at the genus level, whereas Fusobacterium was prevalent in soil. The diversity and richness of bacterial communities were more similar between cloacal samples than those observed between either sample type compared with soil. Our results showed that tetracycline and aminoglycoside ARG relative abundance was high across all sample types but significantly increased within feces/air compared to soils/vegetables. Association analysis revealed five potential host genera for ARG/MGE presence among various microbiota populations studied here. Our findings confirm that farms are important sources for the environmental dissemination of pathogens and ARGs.http://www.sciencedirect.com/science/article/pii/S0032579124012434Poultry farmMicrobiomeBioaerosolAntibiotic resistance genesHorizontal gene transferEnvironmental contamination |
| spellingShingle | ShangMin Li Kai Wang DanPing Wang HongZhi Wang HuaXuan Zhao JunHua Pu KeHua Wang ChunMei Li Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems Poultry Science Poultry farm Microbiome Bioaerosol Antibiotic resistance genes Horizontal gene transfer Environmental contamination |
| title | Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems |
| title_full | Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems |
| title_fullStr | Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems |
| title_full_unstemmed | Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems |
| title_short | Distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems |
| title_sort | distribution and environmental dissemination of antibiotic resistance genes in poultry farms and surrounding ecosystems |
| topic | Poultry farm Microbiome Bioaerosol Antibiotic resistance genes Horizontal gene transfer Environmental contamination |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124012434 |
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