Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway
IntroductionFatty acid metabolism plays a crucial role in regulating airway inflammation through the synthesis of lipid mediators. We have previously demonstrated that a 12/15-lipoxygenase (12/15-LOX or Alox15)-derived mediator attenuates IL-33-induced eosinophilic airway inflammation in mice. Howev...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1565670/full |
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| author | Emi Ito Emi Ito Reika Hayashizaki Reika Hayashizaki Takuro Hosaka Takuro Hosaka Tsuyoshi Yamane Jun Miyata Jun Miyata Yosuke Isobe Yosuke Isobe Yosuke Isobe Makoto Arita Makoto Arita Makoto Arita |
| author_facet | Emi Ito Emi Ito Reika Hayashizaki Reika Hayashizaki Takuro Hosaka Takuro Hosaka Tsuyoshi Yamane Jun Miyata Jun Miyata Yosuke Isobe Yosuke Isobe Yosuke Isobe Makoto Arita Makoto Arita Makoto Arita |
| author_sort | Emi Ito |
| collection | DOAJ |
| description | IntroductionFatty acid metabolism plays a crucial role in regulating airway inflammation through the synthesis of lipid mediators. We have previously demonstrated that a 12/15-lipoxygenase (12/15-LOX or Alox15)-derived mediator attenuates IL-33-induced eosinophilic airway inflammation in mice. However, the cellular sources of these mediators remain unclear.MethodsTo identify the cellular sources, we used several cell type-specific conditional 12/15-LOX-deficient mice.ResultsWe found that eosinophils and pleural macrophages were the major 12/15-LOX-expressing cell types responsible for attenuating airway inflammation. Eosinophils were the major population of 12/15-LOX-expressing cells found in inflamed lung tissue. In addition, pleural macrophages were the major population of 12/15-LOX-expressing cells in the thoracic cavity and were found to translocate into inflamed lung tissue in response to airway inflammation.DiscussionThis study suggests that eosinophils and pleural macrophages cooperatively regulate eosinophilic airway inflammation via 12/15-LOX expression. Targeting 12/15-LOX metabolism in these cells may offer new therapeutic strategies for severe asthma. |
| format | Article |
| id | doaj-art-0ee28fef99f0410b98d7f7af2a971191 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-0ee28fef99f0410b98d7f7af2a9711912025-08-20T03:10:28ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.15656701565670Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathwayEmi Ito0Emi Ito1Reika Hayashizaki2Reika Hayashizaki3Takuro Hosaka4Takuro Hosaka5Tsuyoshi Yamane6Jun Miyata7Jun Miyata8Yosuke Isobe9Yosuke Isobe10Yosuke Isobe11Makoto Arita12Makoto Arita13Makoto Arita14Division of Physiological Chemistry and Metabolism, Keio University Faculty of Pharmacy, Tokyo, JapanLaboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanDivision of Physiological Chemistry and Metabolism, Keio University Faculty of Pharmacy, Tokyo, JapanLaboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanDivision of Physiological Chemistry and Metabolism, Keio University Faculty of Pharmacy, Tokyo, JapanLaboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanLaboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanLaboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanDivision of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo, JapanDivision of Physiological Chemistry and Metabolism, Keio University Faculty of Pharmacy, Tokyo, JapanLaboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanCellular and Molecular Epigenetics Laboratory, Graduate School of Medical Life Science, Yokohama City University, Kanagawa, JapanDivision of Physiological Chemistry and Metabolism, Keio University Faculty of Pharmacy, Tokyo, JapanLaboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Kanagawa, JapanCellular and Molecular Epigenetics Laboratory, Graduate School of Medical Life Science, Yokohama City University, Kanagawa, JapanIntroductionFatty acid metabolism plays a crucial role in regulating airway inflammation through the synthesis of lipid mediators. We have previously demonstrated that a 12/15-lipoxygenase (12/15-LOX or Alox15)-derived mediator attenuates IL-33-induced eosinophilic airway inflammation in mice. However, the cellular sources of these mediators remain unclear.MethodsTo identify the cellular sources, we used several cell type-specific conditional 12/15-LOX-deficient mice.ResultsWe found that eosinophils and pleural macrophages were the major 12/15-LOX-expressing cell types responsible for attenuating airway inflammation. Eosinophils were the major population of 12/15-LOX-expressing cells found in inflamed lung tissue. In addition, pleural macrophages were the major population of 12/15-LOX-expressing cells in the thoracic cavity and were found to translocate into inflamed lung tissue in response to airway inflammation.DiscussionThis study suggests that eosinophils and pleural macrophages cooperatively regulate eosinophilic airway inflammation via 12/15-LOX expression. Targeting 12/15-LOX metabolism in these cells may offer new therapeutic strategies for severe asthma.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1565670/fulleosinophilspleural macrophages12/15-lipoxygenaseIL-33lipidomics |
| spellingShingle | Emi Ito Emi Ito Reika Hayashizaki Reika Hayashizaki Takuro Hosaka Takuro Hosaka Tsuyoshi Yamane Jun Miyata Jun Miyata Yosuke Isobe Yosuke Isobe Yosuke Isobe Makoto Arita Makoto Arita Makoto Arita Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway Frontiers in Immunology eosinophils pleural macrophages 12/15-lipoxygenase IL-33 lipidomics |
| title | Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway |
| title_full | Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway |
| title_fullStr | Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway |
| title_full_unstemmed | Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway |
| title_short | Eosinophils and pleural macrophages counter regulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway |
| title_sort | eosinophils and pleural macrophages counter regulate il 33 elicited airway inflammation via the 12 15 lipoxygenase pathway |
| topic | eosinophils pleural macrophages 12/15-lipoxygenase IL-33 lipidomics |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1565670/full |
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