Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensis
The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract of Spirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis us...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2015/132675 |
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| author | Gaurav Sharma Nakuleshwar Dut Jasuja Manoj Kumar Mohammad Irfan Ali |
| author_facet | Gaurav Sharma Nakuleshwar Dut Jasuja Manoj Kumar Mohammad Irfan Ali |
| author_sort | Gaurav Sharma |
| collection | DOAJ |
| description | The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract of Spirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract of S. platensis showed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is, Escherichia coli, MTCC-9721; Proteus vulgaris, MTCC-7299; Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is, Staphylococcus aureus, MTCC-9542; S. epidermidis, MTCC-2639; Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is 31.3±1.11 in P. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications. |
| format | Article |
| id | doaj-art-0eac586d651d4baaa203c2804ee0717f |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-0eac586d651d4baaa203c2804ee0717f2025-02-03T05:44:48ZengWileyJournal of Nanotechnology1687-95031687-95112015-01-01201510.1155/2015/132675132675Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensisGaurav Sharma0Nakuleshwar Dut Jasuja1Manoj Kumar2Mohammad Irfan Ali3School of Sciences, Suresh Gyan Vihar University, Rajasthan 302017, IndiaSchool of Sciences, Suresh Gyan Vihar University, Rajasthan 302017, IndiaMarine Biotechnology Laboratory, Department of Botany, University of Delhi, Delhi, IndiaSchool of Sciences, Suresh Gyan Vihar University, Rajasthan 302017, IndiaThe present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract of Spirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract of S. platensis showed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is, Escherichia coli, MTCC-9721; Proteus vulgaris, MTCC-7299; Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is, Staphylococcus aureus, MTCC-9542; S. epidermidis, MTCC-2639; Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is 31.3±1.11 in P. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.http://dx.doi.org/10.1155/2015/132675 |
| spellingShingle | Gaurav Sharma Nakuleshwar Dut Jasuja Manoj Kumar Mohammad Irfan Ali Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensis Journal of Nanotechnology |
| title | Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensis |
| title_full | Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensis |
| title_fullStr | Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensis |
| title_full_unstemmed | Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensis |
| title_short | Biological Synthesis of Silver Nanoparticles by Cell-Free Extract of Spirulina platensis |
| title_sort | biological synthesis of silver nanoparticles by cell free extract of spirulina platensis |
| url | http://dx.doi.org/10.1155/2015/132675 |
| work_keys_str_mv | AT gauravsharma biologicalsynthesisofsilvernanoparticlesbycellfreeextractofspirulinaplatensis AT nakuleshwardutjasuja biologicalsynthesisofsilvernanoparticlesbycellfreeextractofspirulinaplatensis AT manojkumar biologicalsynthesisofsilvernanoparticlesbycellfreeextractofspirulinaplatensis AT mohammadirfanali biologicalsynthesisofsilvernanoparticlesbycellfreeextractofspirulinaplatensis |