Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation
Acute lymphoblastic leukemia (T-ALL) is one of the most common hematologic malignancies in children worldwide. Despite advances in chemotherapy in recent years, more than 50% of adult T-ALL patients still experience treatment resistance and relapse/refractory disease. Moringa oleifera, a new food re...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1546189/full |
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| author | Xiaoxue Liu Hao Yang Shanna Xie Xinyu Wang Yang Tian Yang Tian Shuang Song Shuang Song Shuang Song |
| author_facet | Xiaoxue Liu Hao Yang Shanna Xie Xinyu Wang Yang Tian Yang Tian Shuang Song Shuang Song Shuang Song |
| author_sort | Xiaoxue Liu |
| collection | DOAJ |
| description | Acute lymphoblastic leukemia (T-ALL) is one of the most common hematologic malignancies in children worldwide. Despite advances in chemotherapy in recent years, more than 50% of adult T-ALL patients still experience treatment resistance and relapse/refractory disease. Moringa oleifera, a new food resource in China, has high nutritional value and various pharmacological activities. Moringa oleifera protein is one of the main active ingredients in Moringa oleifera leaves, and some studies have shown that Moringa oleifera protein has antioxidant, anti-inflammatory, antibacterial, anticancer, and other bioactivities; however, the anti-acute lymphoblastic leukemia (T-ALL) activity of the Moringa oleifera protein and its mechanism of action are still unclear. In this study, we used Moringa oleifera leaves protein as the experimental material and used the MTT assay, flow cytometry, and Western blot techniques to study the effects of Moringa oleifera leaves protein on Jurkat cell growth, apoptosis, and the cell cycle in vitro and the underlying molecular mechanism. Moringa oleifera leaves protein inhibited Jurkat cell proliferation; induced apoptosis and cycle arrest; increased autophagy; and inhibited Jurkat cell proliferation by regulating the MAPK/AKT pathway. The results of this study provide new ideas and a theoretical basis for the development of Moringa oleifera leaves protein for the prevention and treatment of leukemia. |
| format | Article |
| id | doaj-art-0e8d5891b9634b1fb34fb9854c8eb930 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-0e8d5891b9634b1fb34fb9854c8eb9302025-08-20T03:06:48ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.15461891546189Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulationXiaoxue Liu0Hao Yang1Shanna Xie2Xinyu Wang3Yang Tian4Yang Tian5Shuang Song6Shuang Song7Shuang Song8College of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaAcute lymphoblastic leukemia (T-ALL) is one of the most common hematologic malignancies in children worldwide. Despite advances in chemotherapy in recent years, more than 50% of adult T-ALL patients still experience treatment resistance and relapse/refractory disease. Moringa oleifera, a new food resource in China, has high nutritional value and various pharmacological activities. Moringa oleifera protein is one of the main active ingredients in Moringa oleifera leaves, and some studies have shown that Moringa oleifera protein has antioxidant, anti-inflammatory, antibacterial, anticancer, and other bioactivities; however, the anti-acute lymphoblastic leukemia (T-ALL) activity of the Moringa oleifera protein and its mechanism of action are still unclear. In this study, we used Moringa oleifera leaves protein as the experimental material and used the MTT assay, flow cytometry, and Western blot techniques to study the effects of Moringa oleifera leaves protein on Jurkat cell growth, apoptosis, and the cell cycle in vitro and the underlying molecular mechanism. Moringa oleifera leaves protein inhibited Jurkat cell proliferation; induced apoptosis and cycle arrest; increased autophagy; and inhibited Jurkat cell proliferation by regulating the MAPK/AKT pathway. The results of this study provide new ideas and a theoretical basis for the development of Moringa oleifera leaves protein for the prevention and treatment of leukemia.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1546189/fullMoringa oleifera leaves proteinimmunosuppressionJurkat cellscell proliferationMAPK/AKT signaling pathwayautophagy |
| spellingShingle | Xiaoxue Liu Hao Yang Shanna Xie Xinyu Wang Yang Tian Yang Tian Shuang Song Shuang Song Shuang Song Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation Frontiers in Immunology Moringa oleifera leaves protein immunosuppression Jurkat cells cell proliferation MAPK/AKT signaling pathway autophagy |
| title | Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation |
| title_full | Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation |
| title_fullStr | Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation |
| title_full_unstemmed | Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation |
| title_short | Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation |
| title_sort | moringa oleifera leaves protein suppresses t lymphoblastic leukemogenesis via mapk akt signaling modulation of apoptotic activation and autophagic flux regulation |
| topic | Moringa oleifera leaves protein immunosuppression Jurkat cells cell proliferation MAPK/AKT signaling pathway autophagy |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1546189/full |
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