ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION

Eighty three local isolates of fungi were isolated from different resources (Peanuts , maize, rice, wheat, bread , domestic cheese of sheep, Milk local Cream, Iranian cream, Roquefort cheese and soil). These isolates were purified and identified, it include 14 isolates of Aspergillus flavus, 13 Aspe...

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Main Authors: Amal K. G. AL-Asady, Alaa G.E. AL-Hashimi, Nora T.Y. AL-Temime
Format: Article
Language:English
Published: College of Veterinary Medicine, University of Basrah, Iraq 2017-06-01
Series:Basrah Journal of Veterinary Research
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Online Access:https://bjvr.uobasrah.edu.iq/article_127442_3e40f2ff2ed89f3cc7506c83b547915a.pdf
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author Amal K. G. AL-Asady
Alaa G.E. AL-Hashimi
Nora T.Y. AL-Temime
author_facet Amal K. G. AL-Asady
Alaa G.E. AL-Hashimi
Nora T.Y. AL-Temime
author_sort Amal K. G. AL-Asady
collection DOAJ
description Eighty three local isolates of fungi were isolated from different resources (Peanuts , maize, rice, wheat, bread , domestic cheese of sheep, Milk local Cream, Iranian cream, Roquefort cheese and soil). These isolates were purified and identified, it include 14 isolates of Aspergillus flavus, 13 Aspergillus niger,8 Aspergillus terreus,3Aspergillus parasaticus,3Alternaria spp.,15Penicilliumspp.,7Fusarium spp.,5 Trichoderma spp., 11 Rhizopus spp. and 7Mucor spp. The ability of isolates for producing aflatoxin were tested, the toxic isolates(Aspergillus flavus,Aspergillus terreus ,and Aspergillus parasaticus) were removed.Aspergillus niger which was isolated from maize was choosing as the best lipoxygenase producer after Primary and secondary screening. The growth of the selected isolate colonies had the largest proportion than the Colonies of Penicillium sp. and Trichoderma sp..all so the same isolate had high enzymatic activity 801.4units/ml, while Penicillium sp. and Trichoderma sp. had (559.2 and 120) units/ml respectively.
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institution OA Journals
issn 1813-8497
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language English
publishDate 2017-06-01
publisher College of Veterinary Medicine, University of Basrah, Iraq
record_format Article
series Basrah Journal of Veterinary Research
spelling doaj-art-0e77fb10d8b04f5fbb151ed8878afcff2025-08-20T01:49:57ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562017-06-0116112013510.33762/bvetr.2017.127442127442ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTIONAmal K. G. AL-AsadyAlaa G.E. AL-HashimiNora T.Y. AL-TemimeEighty three local isolates of fungi were isolated from different resources (Peanuts , maize, rice, wheat, bread , domestic cheese of sheep, Milk local Cream, Iranian cream, Roquefort cheese and soil). These isolates were purified and identified, it include 14 isolates of Aspergillus flavus, 13 Aspergillus niger,8 Aspergillus terreus,3Aspergillus parasaticus,3Alternaria spp.,15Penicilliumspp.,7Fusarium spp.,5 Trichoderma spp., 11 Rhizopus spp. and 7Mucor spp. The ability of isolates for producing aflatoxin were tested, the toxic isolates(Aspergillus flavus,Aspergillus terreus ,and Aspergillus parasaticus) were removed.Aspergillus niger which was isolated from maize was choosing as the best lipoxygenase producer after Primary and secondary screening. The growth of the selected isolate colonies had the largest proportion than the Colonies of Penicillium sp. and Trichoderma sp..all so the same isolate had high enzymatic activity 801.4units/ml, while Penicillium sp. and Trichoderma sp. had (559.2 and 120) units/ml respectively.https://bjvr.uobasrah.edu.iq/article_127442_3e40f2ff2ed89f3cc7506c83b547915a.pdffungiaspergillus nigerlipoxygenase
spellingShingle Amal K. G. AL-Asady
Alaa G.E. AL-Hashimi
Nora T.Y. AL-Temime
ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION
Basrah Journal of Veterinary Research
fungi
aspergillus niger
lipoxygenase
title ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION
title_full ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION
title_fullStr ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION
title_full_unstemmed ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION
title_short ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION
title_sort isolation identification of some fugal isolates and testing their ability for lipoxygenase production
topic fungi
aspergillus niger
lipoxygenase
url https://bjvr.uobasrah.edu.iq/article_127442_3e40f2ff2ed89f3cc7506c83b547915a.pdf
work_keys_str_mv AT amalkgalasady isolationidentificationofsomefugalisolatesandtestingtheirabilityforlipoxygenaseproduction
AT alaagealhashimi isolationidentificationofsomefugalisolatesandtestingtheirabilityforlipoxygenaseproduction
AT noratyaltemime isolationidentificationofsomefugalisolatesandtestingtheirabilityforlipoxygenaseproduction