Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells
Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity. In practical application, the research of food combinations is more significant than a specific food component. This study investigated...
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302300143X |
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| author | Yijun Yao Zhifang Li Bowen Qin Xingrong Ju Lifeng Wang |
| author_facet | Yijun Yao Zhifang Li Bowen Qin Xingrong Ju Lifeng Wang |
| author_sort | Yijun Yao |
| collection | DOAJ |
| description | Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity. In practical application, the research of food combinations is more significant than a specific food component. This study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid (OA). Five indexes, triglyceride (TG), total cholesterol (T-CHO), low density lipoprotein-cholesterol (LDL-C), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), were used to evaluate the lipid-lowering effect of highland barley extract. We also preliminary studied the lipid-lowering mechanism by Real-time fluorescent quantitative polymerase chain reaction (qPCR). The results indicated that highland barley extract contains many components with lipid-lowering effects, such as hyperoside and scoparone. In vitro, the lipase assay showed an 18.4 % lipase inhibition rate when the additive contents of highland barley extract were 100 µg/mL. The intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mM OA-induced HepG2 cells. The results showed that intracellular TG, LDL-C, and T-CHO content decreased by 34.4 %, 51.2 %, and 18.4 %, respectively. ALT and AST decreased by 51.6 % and 20.7 % compared with the untreated hyperlipidemic HepG2 cells. qPCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγ and Fabp4. |
| format | Article |
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| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-0e57819e6a76402d9bd3eec36cc7f0e42025-02-03T04:09:17ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131454461Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cellsYijun Yao0Zhifang Li1Bowen Qin2Xingrong Ju3Lifeng Wang4College of Food Science and Engineering/ Collaborative Innovation Centre for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, ChinaCollege of Food Science and Engineering/ Collaborative Innovation Centre for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, ChinaCollege of Food Science and Engineering/ Collaborative Innovation Centre for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, ChinaCollege of Food Science and Engineering/ Collaborative Innovation Centre for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, ChinaCorresponding author at: College of Food Science and Engineering/ Collaborative Innovation Centre for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, China.; College of Food Science and Engineering/ Collaborative Innovation Centre for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210023, ChinaActive ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity. In practical application, the research of food combinations is more significant than a specific food component. This study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid (OA). Five indexes, triglyceride (TG), total cholesterol (T-CHO), low density lipoprotein-cholesterol (LDL-C), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), were used to evaluate the lipid-lowering effect of highland barley extract. We also preliminary studied the lipid-lowering mechanism by Real-time fluorescent quantitative polymerase chain reaction (qPCR). The results indicated that highland barley extract contains many components with lipid-lowering effects, such as hyperoside and scoparone. In vitro, the lipase assay showed an 18.4 % lipase inhibition rate when the additive contents of highland barley extract were 100 µg/mL. The intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mM OA-induced HepG2 cells. The results showed that intracellular TG, LDL-C, and T-CHO content decreased by 34.4 %, 51.2 %, and 18.4 %, respectively. ALT and AST decreased by 51.6 % and 20.7 % compared with the untreated hyperlipidemic HepG2 cells. qPCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγ and Fabp4.http://www.sciencedirect.com/science/article/pii/S221345302300143XHighland barleyPolyphenols extractLipid-lowering effectHepG2 cells |
| spellingShingle | Yijun Yao Zhifang Li Bowen Qin Xingrong Ju Lifeng Wang Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells Food Science and Human Wellness Highland barley Polyphenols extract Lipid-lowering effect HepG2 cells |
| title | Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells |
| title_full | Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells |
| title_fullStr | Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells |
| title_full_unstemmed | Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells |
| title_short | Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells |
| title_sort | evaluation of the intracellular lipid lowering effect of polyphenols extract from highland barley in hepg2 cells |
| topic | Highland barley Polyphenols extract Lipid-lowering effect HepG2 cells |
| url | http://www.sciencedirect.com/science/article/pii/S221345302300143X |
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