A preliminary evaluation of a fast, low-cost, and high-throughput nucleic acid extraction method for bacterial microbiota profiling in low-microbial biomass samples

A preliminary evaluation of a fast, low-cost, and high-throughput nucleic acid extraction method for bacterial microbiota profiling in low-microbial biomass samples

Abstract The respiratory tract is colonized with low-density microbial communities, which have been shown to impact human respiratory health through microbiota–host interactions. However, a lack of fast and cost-effective nucleic acid extraction method for low-microbial biomass samples hinders inves...

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Bibliographic Details
Main Authors: Xiangning Bai, Simon Rayner, Linda Ellevog Skuggen, Kine Kragl Engseth, Pål Marius Bjørnstad, Marianne Dalland, Gregor D. Gilfillan, Magnar Bjørås, Andreas Matussek
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
Microbiota
Bacteria
16S rRNA gene sequencing
Low-microbial biomass
Respiratory samples
Nucleic acid extraction
Online Access:https://doi.org/10.1038/s41598-024-84682-9
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