Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study
The aim of this study was to investigate the loss of docosahexaenoic acid (DHA) from three supplements (two powders and one oil) after digestion (rumen and gastrointestinal) and their effects on the number and composition of rumen bacteria, using an in vitro approach. The concentration of supplement...
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2025-01-01
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| author | Jianmin Zou Genna Ba Dian Wang Mengmeng Li Shaohong Jin Chong Chen Wei Tan Jian He Hengsheng Du Pengjie Wang Yinhua Zhu |
| author_facet | Jianmin Zou Genna Ba Dian Wang Mengmeng Li Shaohong Jin Chong Chen Wei Tan Jian He Hengsheng Du Pengjie Wang Yinhua Zhu |
| author_sort | Jianmin Zou |
| collection | DOAJ |
| description | The aim of this study was to investigate the loss of docosahexaenoic acid (DHA) from three supplements (two powders and one oil) after digestion (rumen and gastrointestinal) and their effects on the number and composition of rumen bacteria, using an in vitro approach. The concentration of supplements has a significant impact on the DHA loss rate and algal oil exhibited the highest rate of loss, but bioaccessibility was not significantly different from the other supplements. 16S rRNA sequencing showed that three DHA supplements altered the bacterial composition of in vitro batch cultures inoculated with rumen microorganisms from cows, and caused changes in the relative abundance of important bacterial phyla, families, and genera. DHA supplements altered the abundance of bacterial species, including <i>Prevotella</i>, <i>Ruminobacter</i>, <i>Succiniclassicum</i>, <i>Succinivibrio</i>, <i>Lachnospiraceae</i>, and <i>Muribaculaceae</i>. Importantly, these changes may be associated with the ruminal response in biohydrogenation. Algal oil has the most significant impact on rumen microbiota by reducing the richness and diversity of rumen microbiota, and significantly altering the composition of multiple important microbiota. |
| format | Article |
| id | doaj-art-0e38ded45afc4166a024a86bc57ee708 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-0e38ded45afc4166a024a86bc57ee7082025-01-24T13:18:01ZengMDPI AGAnimals2076-26152025-01-0115219610.3390/ani15020196Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro StudyJianmin Zou0Genna Ba1Dian Wang2Mengmeng Li3Shaohong Jin4Chong Chen5Wei Tan6Jian He7Hengsheng Du8Pengjie Wang9Yinhua Zhu10School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, ChinaInner Mongolia Yili Industrial Group, Co., Ltd., Hohhot 010110, ChinaInner Mongolia Youran Dairy Group Limited, Hohhot 010010, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaDepartment of Nutrition and Health, China Agricultural University, No. 17 Tsinghua East Road, Haidian District, Beijing 100083, ChinaDepartment of Nutrition and Health, China Agricultural University, No. 17 Tsinghua East Road, Haidian District, Beijing 100083, ChinaInner Mongolia Yili Industrial Group, Co., Ltd., Hohhot 010110, ChinaNational Center of Technology Innovation for Dairy, Hohhot 010110, ChinaNational Center of Technology Innovation for Dairy, Hohhot 010110, ChinaDepartment of Nutrition and Health, China Agricultural University, No. 17 Tsinghua East Road, Haidian District, Beijing 100083, ChinaDepartment of Nutrition and Health, China Agricultural University, No. 17 Tsinghua East Road, Haidian District, Beijing 100083, ChinaThe aim of this study was to investigate the loss of docosahexaenoic acid (DHA) from three supplements (two powders and one oil) after digestion (rumen and gastrointestinal) and their effects on the number and composition of rumen bacteria, using an in vitro approach. The concentration of supplements has a significant impact on the DHA loss rate and algal oil exhibited the highest rate of loss, but bioaccessibility was not significantly different from the other supplements. 16S rRNA sequencing showed that three DHA supplements altered the bacterial composition of in vitro batch cultures inoculated with rumen microorganisms from cows, and caused changes in the relative abundance of important bacterial phyla, families, and genera. DHA supplements altered the abundance of bacterial species, including <i>Prevotella</i>, <i>Ruminobacter</i>, <i>Succiniclassicum</i>, <i>Succinivibrio</i>, <i>Lachnospiraceae</i>, and <i>Muribaculaceae</i>. Importantly, these changes may be associated with the ruminal response in biohydrogenation. Algal oil has the most significant impact on rumen microbiota by reducing the richness and diversity of rumen microbiota, and significantly altering the composition of multiple important microbiota.https://www.mdpi.com/2076-2615/15/2/196in vitro digestionDHA supplementsrumen bacterial communityDHA bioaccessibility |
| spellingShingle | Jianmin Zou Genna Ba Dian Wang Mengmeng Li Shaohong Jin Chong Chen Wei Tan Jian He Hengsheng Du Pengjie Wang Yinhua Zhu Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study Animals in vitro digestion DHA supplements rumen bacterial community DHA bioaccessibility |
| title | Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study |
| title_full | Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study |
| title_fullStr | Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study |
| title_full_unstemmed | Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study |
| title_short | Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study |
| title_sort | rumen bacterial community responses to three dha supplements a comparative in vitro study |
| topic | in vitro digestion DHA supplements rumen bacterial community DHA bioaccessibility |
| url | https://www.mdpi.com/2076-2615/15/2/196 |
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