Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans
Unseparated peripheral blood mononuclear cells (PBMCs) obtained from drug-naïve African individuals living in a context of multi-infections and presenting with high viral load (VL), were cultured in vitro and tested for their ability to produce antibodies (Abs)...
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| Format: | Article |
| Language: | English |
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Wiley
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/10446670410001722104 |
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| author | Lydie Béniguel Evelyne Bégaud Fabrice Cognasse Philippe Gabrié Christophe D. Mbolidi Mary A. Marovich Céline Cazorla Frédéric Lucht Christian Genin Olivier Garraud |
| author_facet | Lydie Béniguel Evelyne Bégaud Fabrice Cognasse Philippe Gabrié Christophe D. Mbolidi Mary A. Marovich Céline Cazorla Frédéric Lucht Christian Genin Olivier Garraud |
| author_sort | Lydie Béniguel |
| collection | DOAJ |
| description | Unseparated peripheral blood mononuclear cells (PBMCs) obtained from
drug-naïve African individuals living in a context of multi-infections and presenting
with high viral load (VL), were cultured in vitro and tested for their ability to produce
antibodies (Abs) reacting with HIV-1 antigens. Within these PBMCs, circulating
B cells were differentiated in vitro and produced IgG Abs against not only ENV, but
also GAG and POL proteins. Under similar experimental conditions, HAART treated
patients produced Abs to ENV proteins only. The in vitro antibody production by
drug-naïve individuals' PBMCs depended on exogenous cytokines (IL-2 and IL-10)
but neither on the re-stimulation of reactive cells in cultures by purified HIV-1-gp
160 antigen nor on the re-engagement of CD40 surface molecules. Further, it was
not abrogated by the addition of various monoclonal Abs (mAbs) to co-stimulatory
molecules. This suggests that the in vitro antibody production by drug-naïve
individuals' PBMCs resulted from the maturation of already envelope and core
antigen-primed, differentiated B cells, presumably pre-plasma cells, which are
not known to circulate at homeostasy. As in vitro produced Abs retained the
capacity of binding antigen and forming complexes, this study provides
pre-clinical support for functional humoral
responses despite major HIV- and other tropical pathogen-induced B cell
perturbations. |
| format | Article |
| id | doaj-art-0e1ea0ae1a0746bd85b739f5dd210df2 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-0e1ea0ae1a0746bd85b739f5dd210df22025-02-03T05:48:07ZengWileyClinical and Developmental Immunology1740-25221740-25302004-01-0111212112710.1080/10446670410001722104Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected AfricansLydie Béniguel0Evelyne Bégaud1Fabrice Cognasse2Philippe Gabrié3Christophe D. Mbolidi4Mary A. Marovich5Céline Cazorla6Frédéric Lucht7Christian Genin8Olivier Garraud9GIMAP EA3064, Faculté de Médecine, Université J. Monnet, St-Etienne, France Institut Pasteur de Bangui, Bangui, Central African RepublicGIMAP EA3064, Faculté de Médecine, Université J. Monnet, St-Etienne, FranceHôpital Communautaire, Bangui, Central African RepublicHôpital Communautaire, Bangui, Central African RepublicCombined US Military HIV Research Program, Rockville, MD, USAService des Maladies Infectieuses, CHU. St-Etienne, FranceService des Maladies Infectieuses, CHU. St-Etienne, FranceGIMAP EA3064, Faculté de Médecine, Université J. Monnet, St-Etienne, FranceGIMAP EA3064, Faculté de Médecine, Université J. Monnet, St-Etienne, FranceUnseparated peripheral blood mononuclear cells (PBMCs) obtained from drug-naïve African individuals living in a context of multi-infections and presenting with high viral load (VL), were cultured in vitro and tested for their ability to produce antibodies (Abs) reacting with HIV-1 antigens. Within these PBMCs, circulating B cells were differentiated in vitro and produced IgG Abs against not only ENV, but also GAG and POL proteins. Under similar experimental conditions, HAART treated patients produced Abs to ENV proteins only. The in vitro antibody production by drug-naïve individuals' PBMCs depended on exogenous cytokines (IL-2 and IL-10) but neither on the re-stimulation of reactive cells in cultures by purified HIV-1-gp 160 antigen nor on the re-engagement of CD40 surface molecules. Further, it was not abrogated by the addition of various monoclonal Abs (mAbs) to co-stimulatory molecules. This suggests that the in vitro antibody production by drug-naïve individuals' PBMCs resulted from the maturation of already envelope and core antigen-primed, differentiated B cells, presumably pre-plasma cells, which are not known to circulate at homeostasy. As in vitro produced Abs retained the capacity of binding antigen and forming complexes, this study provides pre-clinical support for functional humoral responses despite major HIV- and other tropical pathogen-induced B cell perturbations.http://dx.doi.org/10.1080/10446670410001722104 |
| spellingShingle | Lydie Béniguel Evelyne Bégaud Fabrice Cognasse Philippe Gabrié Christophe D. Mbolidi Mary A. Marovich Céline Cazorla Frédéric Lucht Christian Genin Olivier Garraud Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans Clinical and Developmental Immunology |
| title | Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans |
| title_full | Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans |
| title_fullStr | Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans |
| title_full_unstemmed | Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans |
| title_short | Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans |
| title_sort | specific antibody production by blood b cells is retained in late stage drug naive hiv infected africans |
| url | http://dx.doi.org/10.1080/10446670410001722104 |
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