Specific Antibody Production by Blood B Cells is Retained in Late Stage Drug-naïve HIV-infected Africans
Unseparated peripheral blood mononuclear cells (PBMCs) obtained from drug-naïve African individuals living in a context of multi-infections and presenting with high viral load (VL), were cultured in vitro and tested for their ability to produce antibodies (Abs)...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/10446670410001722104 |
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| Summary: | Unseparated peripheral blood mononuclear cells (PBMCs) obtained from
drug-naïve African individuals living in a context of multi-infections and presenting
with high viral load (VL), were cultured in vitro and tested for their ability to produce
antibodies (Abs) reacting with HIV-1 antigens. Within these PBMCs, circulating
B cells were differentiated in vitro and produced IgG Abs against not only ENV, but
also GAG and POL proteins. Under similar experimental conditions, HAART treated
patients produced Abs to ENV proteins only. The in vitro antibody production by
drug-naïve individuals' PBMCs depended on exogenous cytokines (IL-2 and IL-10)
but neither on the re-stimulation of reactive cells in cultures by purified HIV-1-gp
160 antigen nor on the re-engagement of CD40 surface molecules. Further, it was
not abrogated by the addition of various monoclonal Abs (mAbs) to co-stimulatory
molecules. This suggests that the in vitro antibody production by drug-naïve
individuals' PBMCs resulted from the maturation of already envelope and core
antigen-primed, differentiated B cells, presumably pre-plasma cells, which are
not known to circulate at homeostasy. As in vitro produced Abs retained the
capacity of binding antigen and forming complexes, this study provides
pre-clinical support for functional humoral
responses despite major HIV- and other tropical pathogen-induced B cell
perturbations. |
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| ISSN: | 1740-2522 1740-2530 |