Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of Morocco
This study aimed to characterize endophytic bacteria isolated from legume nodules and roots in the rhizosphere soils of Acacia trees in Morocco’s arid regions. The focus was on identifying bacterial strains with plant growth-promoting rhizobacteria (PGPR) traits and antibiotic resistance, which coul...
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2024-10-01
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| Series: | International Journal of Plant Biology |
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| author | Khadijattou Taoufiq Laila Aberchane Oukacha Amri Khalid Oufdou Khouzaima El Biari Abdeslem Talbi Rachid Fghire Hassan Ouachtak Mustapha Faghire |
| author_facet | Khadijattou Taoufiq Laila Aberchane Oukacha Amri Khalid Oufdou Khouzaima El Biari Abdeslem Talbi Rachid Fghire Hassan Ouachtak Mustapha Faghire |
| author_sort | Khadijattou Taoufiq |
| collection | DOAJ |
| description | This study aimed to characterize endophytic bacteria isolated from legume nodules and roots in the rhizosphere soils of Acacia trees in Morocco’s arid regions. The focus was on identifying bacterial strains with plant growth-promoting rhizobacteria (PGPR) traits and antibiotic resistance, which could enhance legume productivity under various abiotic stresses. Autochthonous legumes were used to harbor the endophytic bacteria, including chickpea (<i>Cicer arietinum</i>), faba bean (<i>Vicia faba</i>), lentil (<i>Lens culinaris</i>), and common bean (<i>Phaseolus vulgaris</i>). In a previous study, seventy-two isolates were obtained, and molecular characterization grouped them into twenty-two bacterial isolates. These twenty-two bacterial isolates were then further analyzed for their antibiotic resistance and key PGPR traits, such as phosphate solubilization, indole-3-acetic acid (IAA) production, and siderophore production. The results revealed that 86.36% of the isolates were resistant to erythromycin, 45.45% to ciprofloxacin, 22.73% to ampicillin-sulbactam, and 9.09% to tetracycline, with ciprofloxacin and tetracycline being the most effective. All isolates produced IAA, with HN51 and PN105 exhibiting the highest production at 6 µg of IAA per mg of protein. The other isolates showed varying levels of IAA production, ranging from moderate to low. Siderophore production, assessed using CAS medium, indicated that the strains PN121, LR142, LNR146, and HR26 exhibited high production, while the rest demonstrated moderate to low capacities. Additionally, 18.2% of the isolates demonstrated phosphate solubilization on YED-P medium, with PR135 and LNR135 being the most efficient, achieving solubilization indices of 2.14 and 2.13 cm, respectively. LR142 and LNR146 showed a moderate solubilization efficiency. Overall, these findings indicate that these isolated endophytic bacteria possess significant potential as biofertilizers, owing to their antibiotic resistance, IAA production, siderophore production, and phosphate solubilization abilities. These characteristics position them as promising candidates for enhancing legume growth under abiotic stress and contributing to sustainable agriculture in arid regions. |
| format | Article |
| id | doaj-art-0e1758e17ce942dcadc03bd60d752e18 |
| institution | OA Journals |
| issn | 2037-0164 |
| language | English |
| publishDate | 2024-10-01 |
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| series | International Journal of Plant Biology |
| spelling | doaj-art-0e1758e17ce942dcadc03bd60d752e182025-08-20T02:00:28ZengMDPI AGInternational Journal of Plant Biology2037-01642024-10-011541063107610.3390/ijpb15040075Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of MoroccoKhadijattou Taoufiq0Laila Aberchane1Oukacha Amri2Khalid Oufdou3Khouzaima El Biari4Abdeslem Talbi5Rachid Fghire6Hassan Ouachtak7Mustapha Faghire8Laboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences, Ibn Zohr University (UIZ), Agadir 80000, MoroccoLaboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences, Ibn Zohr University (UIZ), Agadir 80000, MoroccoLaboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences, Ibn Zohr University (UIZ), Agadir 80000, MoroccoLaboratory of Microbial Biotechnology, Agrosciences and Environment (BioMAgE), Labeled Research Unit-CNRST N°4, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech 40000, MoroccoLaboratory of Biology, Ecology and Health, Faculty of Sciences of Tetouan, Abdelmalek Essaadi University, Tétouan 93000, MoroccoLaboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences, Ibn Zohr University (UIZ), Agadir 80000, MoroccoDépartement de Biologie, Faculté des Sciences, Université Chouaïb Doukali El Jadida, El Jadida 24000, MoroccoLaboratory of Organic and Physical Chemistry, Faculty of Science, Ibn Zohr University, Agadir 80000, MoroccoLaboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences, Ibn Zohr University (UIZ), Agadir 80000, MoroccoThis study aimed to characterize endophytic bacteria isolated from legume nodules and roots in the rhizosphere soils of Acacia trees in Morocco’s arid regions. The focus was on identifying bacterial strains with plant growth-promoting rhizobacteria (PGPR) traits and antibiotic resistance, which could enhance legume productivity under various abiotic stresses. Autochthonous legumes were used to harbor the endophytic bacteria, including chickpea (<i>Cicer arietinum</i>), faba bean (<i>Vicia faba</i>), lentil (<i>Lens culinaris</i>), and common bean (<i>Phaseolus vulgaris</i>). In a previous study, seventy-two isolates were obtained, and molecular characterization grouped them into twenty-two bacterial isolates. These twenty-two bacterial isolates were then further analyzed for their antibiotic resistance and key PGPR traits, such as phosphate solubilization, indole-3-acetic acid (IAA) production, and siderophore production. The results revealed that 86.36% of the isolates were resistant to erythromycin, 45.45% to ciprofloxacin, 22.73% to ampicillin-sulbactam, and 9.09% to tetracycline, with ciprofloxacin and tetracycline being the most effective. All isolates produced IAA, with HN51 and PN105 exhibiting the highest production at 6 µg of IAA per mg of protein. The other isolates showed varying levels of IAA production, ranging from moderate to low. Siderophore production, assessed using CAS medium, indicated that the strains PN121, LR142, LNR146, and HR26 exhibited high production, while the rest demonstrated moderate to low capacities. Additionally, 18.2% of the isolates demonstrated phosphate solubilization on YED-P medium, with PR135 and LNR135 being the most efficient, achieving solubilization indices of 2.14 and 2.13 cm, respectively. LR142 and LNR146 showed a moderate solubilization efficiency. Overall, these findings indicate that these isolated endophytic bacteria possess significant potential as biofertilizers, owing to their antibiotic resistance, IAA production, siderophore production, and phosphate solubilization abilities. These characteristics position them as promising candidates for enhancing legume growth under abiotic stress and contributing to sustainable agriculture in arid regions.https://www.mdpi.com/2037-0164/15/4/75rhizosphere soil<i>Acacia</i>legumesendophytic bacteriaantibiotics resistanceindole 3-acetic acid |
| spellingShingle | Khadijattou Taoufiq Laila Aberchane Oukacha Amri Khalid Oufdou Khouzaima El Biari Abdeslem Talbi Rachid Fghire Hassan Ouachtak Mustapha Faghire Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of Morocco International Journal of Plant Biology rhizosphere soil <i>Acacia</i> legumes endophytic bacteria antibiotics resistance indole 3-acetic acid |
| title | Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of Morocco |
| title_full | Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of Morocco |
| title_fullStr | Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of Morocco |
| title_full_unstemmed | Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of Morocco |
| title_short | Antibiotics Resistance and PGPR Traits of Endophytic Bacteria Isolated in Arid Region of Morocco |
| title_sort | antibiotics resistance and pgpr traits of endophytic bacteria isolated in arid region of morocco |
| topic | rhizosphere soil <i>Acacia</i> legumes endophytic bacteria antibiotics resistance indole 3-acetic acid |
| url | https://www.mdpi.com/2037-0164/15/4/75 |
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