Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release Applications
This study developed a novel water-soluble Cellulose Acetoacetate (CAA)-chitosan (CS) composite hydrogel drug delivery system. In this system, CAA and CS molecules are cross-linked via dynamic enamine bonds, forming a three-dimensional network structure suitable for drug encapsulation and controlled...
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MDPI AG
2025-05-01
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| author | Xueyan Jin Hong Xu Zhiping Mao Xueling Feng Yi Zhong |
| author_facet | Xueyan Jin Hong Xu Zhiping Mao Xueling Feng Yi Zhong |
| author_sort | Xueyan Jin |
| collection | DOAJ |
| description | This study developed a novel water-soluble Cellulose Acetoacetate (CAA)-chitosan (CS) composite hydrogel drug delivery system. In this system, CAA and CS molecules are cross-linked via dynamic enamine bonds, forming a three-dimensional network structure suitable for drug encapsulation and controlled release. The primary objective was to address the challenges associated with the short half-life and significant fluctuations in therapeutic concentration of cytokine drugs, such as interleukin-2 (IL-2). A hydrogel system with a three-dimensional spatial network structure was successfully constructed via dynamic enamine bonds cross-linking between the acetoacetate groups in CAA molecules and the amino groups in CS. This system exhibits the following characteristics: (1) Dynamic covalent bonds impart adjustable mechanical properties to the hydrogel, enabling precise control over gelation time and mechanical performance; (2) A hierarchical pore structure (average pore size of 100–200 μm) provides a three-dimensional confined space for efficient drug encapsulation, achieving an IL-2 encapsulation efficiency of 83.3 ± 3.1%; (3) In vitro release studies demonstrated that the cumulative release of IL-2 within 72 h ranged from 18.4% to 34.7%, indicating sustained-release behavior. Cell viability assays confirmed that the hydrogel maintained the survival rate of L929 cells above 85% (as determined by the CCK-8 method), and live/dead staining revealed no apparent cytotoxicity. Overall, this three-dimensional network hydrogel based on dynamic covalent bonds represents a promising strategy for low-dose, long-lasting cytokine delivery. |
| format | Article |
| id | doaj-art-0e0b23ea61d045f591cfb51b41d541f0 |
| institution | OA Journals |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-0e0b23ea61d045f591cfb51b41d541f02025-08-20T01:56:17ZengMDPI AGApplied Sciences2076-34172025-05-011510560110.3390/app15105601Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release ApplicationsXueyan Jin0Hong Xu1Zhiping Mao2Xueling Feng3Yi Zhong4A Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, ChinaA Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, ChinaA Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, ChinaA Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, ChinaA Key Lab of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, ChinaThis study developed a novel water-soluble Cellulose Acetoacetate (CAA)-chitosan (CS) composite hydrogel drug delivery system. In this system, CAA and CS molecules are cross-linked via dynamic enamine bonds, forming a three-dimensional network structure suitable for drug encapsulation and controlled release. The primary objective was to address the challenges associated with the short half-life and significant fluctuations in therapeutic concentration of cytokine drugs, such as interleukin-2 (IL-2). A hydrogel system with a three-dimensional spatial network structure was successfully constructed via dynamic enamine bonds cross-linking between the acetoacetate groups in CAA molecules and the amino groups in CS. This system exhibits the following characteristics: (1) Dynamic covalent bonds impart adjustable mechanical properties to the hydrogel, enabling precise control over gelation time and mechanical performance; (2) A hierarchical pore structure (average pore size of 100–200 μm) provides a three-dimensional confined space for efficient drug encapsulation, achieving an IL-2 encapsulation efficiency of 83.3 ± 3.1%; (3) In vitro release studies demonstrated that the cumulative release of IL-2 within 72 h ranged from 18.4% to 34.7%, indicating sustained-release behavior. Cell viability assays confirmed that the hydrogel maintained the survival rate of L929 cells above 85% (as determined by the CCK-8 method), and live/dead staining revealed no apparent cytotoxicity. Overall, this three-dimensional network hydrogel based on dynamic covalent bonds represents a promising strategy for low-dose, long-lasting cytokine delivery.https://www.mdpi.com/2076-3417/15/10/5601hydrogelcelluloseacetoacetatechitosandrug-sustained-release |
| spellingShingle | Xueyan Jin Hong Xu Zhiping Mao Xueling Feng Yi Zhong Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release Applications Applied Sciences hydrogel cellulose acetoacetate chitosan drug-sustained-release |
| title | Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release Applications |
| title_full | Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release Applications |
| title_fullStr | Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release Applications |
| title_full_unstemmed | Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release Applications |
| title_short | Polysaccharide Hydrogels Based on Cellulose and Chitosan for Drug Sustained-Release Applications |
| title_sort | polysaccharide hydrogels based on cellulose and chitosan for drug sustained release applications |
| topic | hydrogel cellulose acetoacetate chitosan drug-sustained-release |
| url | https://www.mdpi.com/2076-3417/15/10/5601 |
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