Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography
A new micellar electrokinetic capillary chromatographic (MEKC) method has been developed and optimized for simultaneous quantitation of doxorubicin (Dox) and fullerenol (Frl) in rat serum. The separation was carried out in a capillary (48.5–40 cm to the detector – 50 µm id fused-silica capillary wit...
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| Format: | Article |
| Language: | English |
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Sciendo
2024-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2024-0026 |
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| author | Injac Rade Sertić Miranda Glavač Nina Kočevar |
| author_facet | Injac Rade Sertić Miranda Glavač Nina Kočevar |
| author_sort | Injac Rade |
| collection | DOAJ |
| description | A new micellar electrokinetic capillary chromatographic (MEKC) method has been developed and optimized for simultaneous quantitation of doxorubicin (Dox) and fullerenol (Frl) in rat serum. The separation was carried out in a capillary (48.5–40 cm to the detector – 50 µm id fused-silica capillary with bubble cell, 150 µm) at an applied voltage of 25 kV and temperature of 25 °C. For the background electrolyte 10 mmol L–1 borate buffer pH 9.3 plus 15 mmol L–1 phosphate buffer pH 7.0 (with the final pH of the mixture adjusted to 7.0 with HCl), with added 10 % (V/V) methanol, and 15 mmol L–1 sodium dodecyl sulfate as a surfactant, were used. The hydrodynamic injection was carried out at 5.0 kPa during the period of 100 s. Linear calibration curves were established over the concentration range 0.5–500.0 mg L–1 for Dox and 10.0–500.0 mg L–1 for Frl (at 234 nm). The proposed MEKC procedure was fully validated and applied for the deter mination of Dox and Frl in Wistar rats after intra pe ritoneal administration of both molecules. |
| format | Article |
| id | doaj-art-0e040ee1b57f4ca4810d597ac80b415f |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Sciendo |
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| series | Acta Pharmaceutica |
| spelling | doaj-art-0e040ee1b57f4ca4810d597ac80b415f2025-02-02T08:34:38ZengSciendoActa Pharmaceutica1846-95582024-09-0174349550910.2478/acph-2024-0026Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatographyInjac Rade0Sertić Miranda1Glavač Nina Kočevar2Faculty of Pharmacy, Chair of Pharmaceutical Biology, University of Ljubljana, 1000Ljubljana, SloveniaFaculty of Pharmacy and Biochemistry Department of Pharmaceutical Analysis, University of Zagreb, 10000ZagrebCroatiaFaculty of Pharmacy, Chair of Pharmaceutical Biology, University of Ljubljana, 1000Ljubljana, SloveniaA new micellar electrokinetic capillary chromatographic (MEKC) method has been developed and optimized for simultaneous quantitation of doxorubicin (Dox) and fullerenol (Frl) in rat serum. The separation was carried out in a capillary (48.5–40 cm to the detector – 50 µm id fused-silica capillary with bubble cell, 150 µm) at an applied voltage of 25 kV and temperature of 25 °C. For the background electrolyte 10 mmol L–1 borate buffer pH 9.3 plus 15 mmol L–1 phosphate buffer pH 7.0 (with the final pH of the mixture adjusted to 7.0 with HCl), with added 10 % (V/V) methanol, and 15 mmol L–1 sodium dodecyl sulfate as a surfactant, were used. The hydrodynamic injection was carried out at 5.0 kPa during the period of 100 s. Linear calibration curves were established over the concentration range 0.5–500.0 mg L–1 for Dox and 10.0–500.0 mg L–1 for Frl (at 234 nm). The proposed MEKC procedure was fully validated and applied for the deter mination of Dox and Frl in Wistar rats after intra pe ritoneal administration of both molecules.https://doi.org/10.2478/acph-2024-0026fullerenoldoxorubicinmekcrat serum |
| spellingShingle | Injac Rade Sertić Miranda Glavač Nina Kočevar Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography Acta Pharmaceutica fullerenol doxorubicin mekc rat serum |
| title | Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography |
| title_full | Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography |
| title_fullStr | Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography |
| title_full_unstemmed | Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography |
| title_short | Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography |
| title_sort | analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography |
| topic | fullerenol doxorubicin mekc rat serum |
| url | https://doi.org/10.2478/acph-2024-0026 |
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