Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo
Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inact...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/4645701 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564311011098624 |
|---|---|
| author | A. A. Lebedeva N. S. Zakharchenko E. V. Trubnikova O. A. Medvedeva T. V. Kuznetsova G. A. Masgutova M. V. Zylkova Y. I. Buryanov A. S. Belous |
| author_facet | A. A. Lebedeva N. S. Zakharchenko E. V. Trubnikova O. A. Medvedeva T. V. Kuznetsova G. A. Masgutova M. V. Zylkova Y. I. Buryanov A. S. Belous |
| author_sort | A. A. Lebedeva |
| collection | DOAJ |
| description | Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S. aureus (equal to Cefazolin) and with a combination of S. aureus with P. aeruginosa (better than Cefazolin). Wild-type K. pinnata extract exhibited evident microbicide activity against S. aureus with P. aeruginosa but it was substantially strengthened in K. pinnata + CecP1 extract. K. pinnata extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type K. pinnata extract accelerated granulation of the wound bed and marginal epithelialization even better than K. pinnata + CecP1 extract. Immunomodulating and microbicide activity of K. pinnata synergizes with microbicide activity of CecP1 accelerating elimination of bacteria. |
| format | Article |
| id | doaj-art-0dcaf7c44e124eb487e896f133e9034c |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-0dcaf7c44e124eb487e896f133e9034c2025-02-03T01:11:17ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/46457014645701Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In VivoA. A. Lebedeva0N. S. Zakharchenko1E. V. Trubnikova2O. A. Medvedeva3T. V. Kuznetsova4G. A. Masgutova5M. V. Zylkova6Y. I. Buryanov7A. S. Belous8Russian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, RussiaRussian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, RussiaKursk State University, Kursk, RussiaKursk State Medical University, Kursk, RussiaNational Institute for Health Development, Tallinn, EstoniaKazan Federal University, Kazan, RussiaRussian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, RussiaRussian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, RussiaKursk State Medical University, Kursk, RussiaProcedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S. aureus (equal to Cefazolin) and with a combination of S. aureus with P. aeruginosa (better than Cefazolin). Wild-type K. pinnata extract exhibited evident microbicide activity against S. aureus with P. aeruginosa but it was substantially strengthened in K. pinnata + CecP1 extract. K. pinnata extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type K. pinnata extract accelerated granulation of the wound bed and marginal epithelialization even better than K. pinnata + CecP1 extract. Immunomodulating and microbicide activity of K. pinnata synergizes with microbicide activity of CecP1 accelerating elimination of bacteria.http://dx.doi.org/10.1155/2017/4645701 |
| spellingShingle | A. A. Lebedeva N. S. Zakharchenko E. V. Trubnikova O. A. Medvedeva T. V. Kuznetsova G. A. Masgutova M. V. Zylkova Y. I. Buryanov A. S. Belous Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo Journal of Immunology Research |
| title | Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo |
| title_full | Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo |
| title_fullStr | Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo |
| title_full_unstemmed | Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo |
| title_short | Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo |
| title_sort | bactericide immunomodulating and wound healing properties of transgenic kalanchoe pinnata synergize with antimicrobial peptide cecropin p1 in vivo |
| url | http://dx.doi.org/10.1155/2017/4645701 |
| work_keys_str_mv | AT aalebedeva bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT nszakharchenko bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT evtrubnikova bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT oamedvedeva bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT tvkuznetsova bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT gamasgutova bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT mvzylkova bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT yiburyanov bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo AT asbelous bactericideimmunomodulatingandwoundhealingpropertiesoftransgenickalanchoepinnatasynergizewithantimicrobialpeptidececropinp1invivo |