The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows
Background/Objectives: The transition from a non-lactating to a lactating state is a critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related to lipid metabolism disorders and is expected to be an effective supplement f...
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2025-01-01
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| author | Zheng Zhou Kang Yong Zhengzhong Luo Zhenlong Du Tao Zhou Xiaoping Li Xueping Yao Liuhong Shen Shumin Yu Yixin Huang Suizhong Cao |
| author_facet | Zheng Zhou Kang Yong Zhengzhong Luo Zhenlong Du Tao Zhou Xiaoping Li Xueping Yao Liuhong Shen Shumin Yu Yixin Huang Suizhong Cao |
| author_sort | Zheng Zhou |
| collection | DOAJ |
| description | Background/Objectives: The transition from a non-lactating to a lactating state is a critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related to lipid metabolism disorders and is expected to be an effective supplement for alleviating excessive lipid mobilisation in periparturient dairy cows. This study aimed to investigate the effects of supplemental DBT on serum biochemical indices, faecal microbial communities, and plasma metabolites in dairy cows. Methods: Thirty cows were randomly divided into three groups: H-DBT group, L-DBT group, and control group. DBT administration was started on the day of calving and continued once daily for seven days. Faecal and blood samples were collected on calving day, 7 days after calving, and 14 days after calving. The levels of serum biochemical indices were measured at three time points in the three groups using commercial kits. Cows in the H-DBT group and control group were selected for metabolome and 16S rRNA amplicon sequencing. Results: Our research shows that, in dairy cows 7 days postpartum, DBT significantly reduced serum 3-hydroxybutyric acid (BHB) concentrations and the number of cows with BHB concentrations ≥ 1 mmol/L. Additionally, DBT increased serum total cholesterol contents at both 7 and 14 days postpartum. Analysis of the microbiota community showed that DBT modulated the composition and structure of the hindgut microbiota. Metabolomic analysis revealed decreased plasma acetylcarnitine, 2-hydroxybutyric acid, and BHB levels 7 days postpartum, whereas the TCA cycle was enhanced. At 14 days postpartum, DBT altered the plasma bile acid profile, especially glycine-conjugated bile acids, including GCDCA, GUDCA, and GDCA. Correlation analyses showed that the relative abundances of <i>Bacillus</i>, <i>Solibacillus, Dorea</i>, and <i>Romboutsia</i> were strongly correlated with the differential metabolites, which is crucial for the beneficial effects of DBT. Conclusions: DBT improves energy status and lipid metabolism in postpartum dairy cows by modulating hindgut microbiota and serum lipid metabolites. |
| format | Article |
| id | doaj-art-0dc90c411749461e8f5e05d24756abcb |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Metabolites |
| spelling | doaj-art-0dc90c411749461e8f5e05d24756abcb2025-01-24T13:41:19ZengMDPI AGMetabolites2218-19892025-01-011515810.3390/metabo15010058The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy CowsZheng Zhou0Kang Yong1Zhengzhong Luo2Zhenlong Du3Tao Zhou4Xiaoping Li5Xueping Yao6Liuhong Shen7Shumin Yu8Yixin Huang9Suizhong Cao10Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Animal Husbandry and Veterinary Medicine, College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404105, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaBackground/Objectives: The transition from a non-lactating to a lactating state is a critical period for lipid metabolism in dairy cows. Danggui Buxue Tang (DBT), stimulating energy metabolism, ameliorates diseases related to lipid metabolism disorders and is expected to be an effective supplement for alleviating excessive lipid mobilisation in periparturient dairy cows. This study aimed to investigate the effects of supplemental DBT on serum biochemical indices, faecal microbial communities, and plasma metabolites in dairy cows. Methods: Thirty cows were randomly divided into three groups: H-DBT group, L-DBT group, and control group. DBT administration was started on the day of calving and continued once daily for seven days. Faecal and blood samples were collected on calving day, 7 days after calving, and 14 days after calving. The levels of serum biochemical indices were measured at three time points in the three groups using commercial kits. Cows in the H-DBT group and control group were selected for metabolome and 16S rRNA amplicon sequencing. Results: Our research shows that, in dairy cows 7 days postpartum, DBT significantly reduced serum 3-hydroxybutyric acid (BHB) concentrations and the number of cows with BHB concentrations ≥ 1 mmol/L. Additionally, DBT increased serum total cholesterol contents at both 7 and 14 days postpartum. Analysis of the microbiota community showed that DBT modulated the composition and structure of the hindgut microbiota. Metabolomic analysis revealed decreased plasma acetylcarnitine, 2-hydroxybutyric acid, and BHB levels 7 days postpartum, whereas the TCA cycle was enhanced. At 14 days postpartum, DBT altered the plasma bile acid profile, especially glycine-conjugated bile acids, including GCDCA, GUDCA, and GDCA. Correlation analyses showed that the relative abundances of <i>Bacillus</i>, <i>Solibacillus, Dorea</i>, and <i>Romboutsia</i> were strongly correlated with the differential metabolites, which is crucial for the beneficial effects of DBT. Conclusions: DBT improves energy status and lipid metabolism in postpartum dairy cows by modulating hindgut microbiota and serum lipid metabolites.https://www.mdpi.com/2218-1989/15/1/58DBTlipid metabolismhindgut microbiotametabolomicsbile acids |
| spellingShingle | Zheng Zhou Kang Yong Zhengzhong Luo Zhenlong Du Tao Zhou Xiaoping Li Xueping Yao Liuhong Shen Shumin Yu Yixin Huang Suizhong Cao The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows Metabolites DBT lipid metabolism hindgut microbiota metabolomics bile acids |
| title | The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows |
| title_full | The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows |
| title_fullStr | The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows |
| title_full_unstemmed | The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows |
| title_short | The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows |
| title_sort | positive regulatory effect of dbt on lipid metabolism in postpartum dairy cows |
| topic | DBT lipid metabolism hindgut microbiota metabolomics bile acids |
| url | https://www.mdpi.com/2218-1989/15/1/58 |
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