Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa
The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Kleb...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/8486742 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547265948942336 |
|---|---|
| author | Sandeep Vasaikar Larry Obi Isaac Morobe Mary Bisi-Johnson |
| author_facet | Sandeep Vasaikar Larry Obi Isaac Morobe Mary Bisi-Johnson |
| author_sort | Sandeep Vasaikar |
| collection | DOAJ |
| description | The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples from patients was obtained from Nelson Mandela Academic Hospital. The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of blaCTX-M, blaTEM, blaSHV, blaKPC, and blaNDM genes. Overall K. pneumoniae were the majority with 169 (83.7%) species isolates, followed by K. oxytoca with 29 (14.4%), while K. ozaenae and Raoultella ornithinolytica were 2 (0.9%) each. The prevalence of ESBL production in all Klebsiella species was 117 (57.9%). ESBL-genotypic resistance is driven in Mthatha by blaSHV 121 (77.1%) followed by blaTEM 105 (66.9%) and blaCTX-M at 89 (56.7%). The most common ESBL genotype combination among the Klebsiella was blaTEM+blaSHV + blaCTX-M at 79 (50.3%). There is a steady increase in the rate of ESBL genes in the last five years. |
| format | Article |
| id | doaj-art-0d82f1dc9de640789e564d41bfaf8b75 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-0d82f1dc9de640789e564d41bfaf8b752025-02-03T06:45:25ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/84867428486742Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South AfricaSandeep Vasaikar0Larry Obi1Isaac Morobe2Mary Bisi-Johnson3Division of Medical Microbiology, Department of Laboratory Medicine and Pathology, Faculty of Health Sciences, Walter Sisulu University, Private Bag X1, Mthatha, Eastern Cape 5117, South AfricaAcademic Affairs, University of Fort Hare, Private Bag X1314, Alice 5700, South AfricaDepartment of Biological Sciences, University of Botswana, Mobutu Drive, P.O. Box 80462, Gaborone, BotswanaDepartment of Microbiology, Obafemi Awolowo University, Ile-Ife, Osun State, NigeriaThe increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples from patients was obtained from Nelson Mandela Academic Hospital. The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of blaCTX-M, blaTEM, blaSHV, blaKPC, and blaNDM genes. Overall K. pneumoniae were the majority with 169 (83.7%) species isolates, followed by K. oxytoca with 29 (14.4%), while K. ozaenae and Raoultella ornithinolytica were 2 (0.9%) each. The prevalence of ESBL production in all Klebsiella species was 117 (57.9%). ESBL-genotypic resistance is driven in Mthatha by blaSHV 121 (77.1%) followed by blaTEM 105 (66.9%) and blaCTX-M at 89 (56.7%). The most common ESBL genotype combination among the Klebsiella was blaTEM+blaSHV + blaCTX-M at 79 (50.3%). There is a steady increase in the rate of ESBL genes in the last five years.http://dx.doi.org/10.1155/2017/8486742 |
| spellingShingle | Sandeep Vasaikar Larry Obi Isaac Morobe Mary Bisi-Johnson Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa International Journal of Microbiology |
| title | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
| title_full | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
| title_fullStr | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
| title_full_unstemmed | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
| title_short | Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa |
| title_sort | molecular characteristics and antibiotic resistance profiles of klebsiella isolates in mthatha eastern cape province south africa |
| url | http://dx.doi.org/10.1155/2017/8486742 |
| work_keys_str_mv | AT sandeepvasaikar molecularcharacteristicsandantibioticresistanceprofilesofklebsiellaisolatesinmthathaeasterncapeprovincesouthafrica AT larryobi molecularcharacteristicsandantibioticresistanceprofilesofklebsiellaisolatesinmthathaeasterncapeprovincesouthafrica AT isaacmorobe molecularcharacteristicsandantibioticresistanceprofilesofklebsiellaisolatesinmthathaeasterncapeprovincesouthafrica AT marybisijohnson molecularcharacteristicsandantibioticresistanceprofilesofklebsiellaisolatesinmthathaeasterncapeprovincesouthafrica |